FastQCFastQC Report
Tue 10 May 2022
EGAF00003662220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7674
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.508209538702111No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA290.37789940057336463No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA180.23455824863174357No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA160.20849622100599427No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA150.1954652071931196No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA140.18243419338024497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.18243419338024497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG130.16940317956737036No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1563721657544957No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA120.1563721657544957No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC120.1563721657544957No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT120.1563721657544957No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA110.14334115194162106No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT110.14334115194162106No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA110.14334115194162106No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG110.14334115194162106No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA90.11727912431587179No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA90.11727912431587179No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC90.11727912431587179No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90.11727912431587179No Hit
CCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTC90.11727912431587179No Hit
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTGCC90.11727912431587179Illumina PCR Primer Index 1 (96% over 28bp)
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG80.10424811050299713Illumina PCR Primer Index 1 (96% over 28bp)
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG80.10424811050299713No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA80.10424811050299713No Hit
GACCAGCACCCATCTCGAGGTGGTATTTTCTGTAAGATCAGGTGTTCCTC80.10424811050299713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT80.10424811050299713No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA80.10424811050299713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10424811050299713No Hit
AAATTAACCTTTGCTCTTGAGAGCAGAAGAGGGAAAGACAGGGGAACATC80.10424811050299713No Hit
GAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA80.10424811050299713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)