FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA59.433962264150944Clontech Universal Primer Mix Long (96% over 26bp)
TTTAGAAATAGGTTATTTGCAGATGCAATCAAGTTAATATGAGGTCATTT35.660377358490567No Hit
GTTTGTGTTCTCAGAATTAATTTAGTTGAACAGTAAACTTGTAGGGCTGT35.660377358490567No Hit
GTAGAAAGAGAATGTTGGGAGCCTTCATTTGATAGAAAAGTAATGTTTCC35.660377358490567No Hit
TAAATAAAACCTTTCAGCCGGGCACAGTGTCTCACGCCTGTAATCCCAAC35.660377358490567No Hit
GTGGTGTTATCTTGGTTCACTGCAGCCTCTGCCTCCTGGGTTCAAGCGAT23.7735849056603774No Hit
GAGGTGGGCAGATCACAAGGTCAGGAGTTCGAGACCAGCCTGACCAACAT23.7735849056603774No Hit
TCGGCGCGCGGCGCTGGAGGGCGGCCAGGGCGTGCCCGACGGCTTGGCCG11.8867924528301887No Hit
GAACAACAAAATTTCCATACAAGAACATATACAGAAAATTATACACATCT11.8867924528301887No Hit
CTGCCTCTGCTGTTCAAAATGTGTCCAGACGCAGCATCCCAAGCCCCCTA11.8867924528301887No Hit
TCCATGTTTAGTGCTTCCTTCAGGAGCTCTTGTAAGGCAGGCCTGGTGGT11.8867924528301887No Hit
ACACACCACCGCGCCCGGCTAATTTTTGTATTTCTACTAGAGATGGGGTT11.8867924528301887No Hit
AATAGAACAGTTCACAACTCAAAAAAGTGGAGGAGTGGAAGAGAAAGAAA11.8867924528301887No Hit
AAATGAGAAAAATCGAGTTCCCAGAGCCGCTACATCAAGAACGGAAAGAA11.8867924528301887No Hit
TAAGTACTTAGTACTTTTCGCCACGGGCACGCAGTTCAGCCAGAACTGTA11.8867924528301887No Hit
ATTCTCACATAAAAATATTGGAAATACTCACCTTATCATGAATGGCCCGC11.8867924528301887No Hit
CCATAACACACACATTCCACATAATACACATATACATCCATACCCTAACA11.8867924528301887No Hit
GCAACCTCTGCCTCCCAGATTCAGGGGACCCTCCTAGCTCAGCCTCCCCA11.8867924528301887No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTC11.8867924528301887No Hit
GTGCCAAGGCCTTGATGCCGTATTGGAAACCGCTGGCTCCGCTGATGCCG11.8867924528301887No Hit
CTCGTTATTTGCGGCTTGACCCAGAGACGGGCGGAAAAATTGCCGTTGCG11.8867924528301887No Hit
CAAGTAGGCTTTATCCCTGTGATGCAAGGTTGGTTCAACATATGCCTGTC11.8867924528301887No Hit
GCATACTCAACGTCTCTTTCGCCTCCCTTTCTCTCGCGACGTTTGACGAC11.8867924528301887No Hit
CTATTAAAAGATAGCTTAATTACGGGGTTATTTAGACATATATCTGTCTC11.8867924528301887No Hit
TGGGCTCTCTCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTTTAA11.8867924528301887No Hit
ACCGAGATCTACATCTTTCCCTACACGACGCTGTCCCAAGTTCTGCAGGT11.8867924528301887Illumina Single End PCR Primer 1 (95% over 21bp)
GAATATTGTGCCCAAATGAAAACGGCTTTTAAAAATTAGCTTCATAATTC11.8867924528301887No Hit
CTTGAGGAGGAGGGAGAAAGGCTTTTGAGTACGGCTTGGGGGCTGTCTCT11.8867924528301887No Hit
GCCGTGCTGCTGTCTCTTGTGCCGGAACAGTCCAGTGCACATGCGTTCTT11.8867924528301887No Hit
GGTCGTGACACCAGCATCGATGGCACGACCCAGCACGCACAACCGAGTCT11.8867924528301887No Hit
CATGAAGGTCCACTGGTTACTTGGCGTACCGATCACCGAGGCCGAGCGTC11.8867924528301887No Hit
GCACGGGCGGTGATAGCATTTGCCCATGAAGTTCTCGACCGCCGCCTTGC11.8867924528301887No Hit
CCATAACACACACATTCCACATAATACACATATACATCCAAACCCTAACA11.8867924528301887No Hit
GCCTATGGCCTGATCGCCGTGCTGGTGGCGTTGACGGGCGGCCTGGGCAA11.8867924528301887No Hit
GCGCCAGACTGTTCGCCGGGACTTCTGCCTCGCTTTCGTGCGTCTCAATC11.8867924528301887No Hit
GCTTCCTTTTGAATGTATCTCTTGTGTATATCAGAAGATAATTGAGAGGG11.8867924528301887No Hit
CTTGAGGAGGAGTGAGAAAGGCTTTTGAGTACGTCTTGGGGGCTGTCTCT11.8867924528301887No Hit
TGGGGTCTCTCTCTCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTTTAA11.8867924528301887No Hit
ATCCTGCATAACTGGTTTTACACTCACAGCAAAAAAAAACCTGAAAAGCA11.8867924528301887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)