FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGACTTCTTTTCTGTAAAAATTAGGAAATTCTAGCATCTTAGCACA35.357142857142857No Hit
AAACGGGAATATCTTCATAGAAAATCTAGACGGAAGCATTCTCAGAAACT35.357142857142857No Hit
TTGCCATGTTGGCCAGGCTGGTCTGGAACTCCTGACCCTGTCTCTTATAC35.357142857142857No Hit
CATCTCACAGAGTTGAAATTTTCTTTTGATTGAGTAGTTTTGAAAAGCTG35.357142857142857No Hit
GGCGATGACGCCGGCGGCGCACAGCAGCAACAGCACGCTGCCGCCCCATA23.571428571428571No Hit
CGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGC23.571428571428571No Hit
ACCTCCGCCTCCTGGGTTCTAGCCATTCTCCTGCCTCGGCCTCCTGAGTA23.571428571428571No Hit
GCATACTCAACGTCTCTTTCGCCTCCCTTTCTCTCGCGACGTTTGACGAC23.571428571428571No Hit
GTGTGGGCCCCACCACGAAAACTTCGGACGAAATCTTTATCATATGTTTG23.571428571428571No Hit
GCCCTGCGCGCGGAACACCAGATCGGGCGCGAGCCCGATCAGGCTGTGCC23.571428571428571No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT23.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
GCCGTCAAATTGCCCCGCCCCCGCCCCGAAGAGGGCATTGCGCAGTGCCT11.7857142857142856No Hit
GATTTCCAGTTTTATTCCACTGTGGTCTGAGAGGATACTTGATATAATTT11.7857142857142856No Hit
CCTCCAAAGCTGCCTAATGCCAGTGCCTCCACCAGGCATCTCCTGCAAGC11.7857142857142856No Hit
TCTCCAAAGTGTCGGTCACCTCCTTTCCATTTCTGCTGTCTCCACTCTGG11.7857142857142856No Hit
ACAATATCCCCACAAGGTCACATTTTGTAATTATAGGATTGCATGGCACT11.7857142857142856No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTACCACCTGTGTA11.7857142857142856No Hit
GAGCGCACCATCGCGAGGCAGGGGCTGGATGCGTTAGGGCAAGCCTTAGG11.7857142857142856No Hit
CTTATGGTGTGACTTCTCAGTTTGCATCATTCTGACTATTGCTGTCTCTT11.7857142857142856No Hit
CCCTCTTCCCAACAAATGCTCTTCCTAACTCCTGCCCAATTCATGTGAGT11.7857142857142856No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.7857142857142856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCGTTATTTGCGGCTTGACCCAGAGACGGGCGGAAAAATTGCCGTTGCG11.7857142857142856No Hit
CTGATCGCCCCTGCTGTCCAGCGCGTGCCCGGCCTCGCCATTGGCGGGCA11.7857142857142856No Hit
GACACAGTGGCATGTGGAGACAACAGTGTGTCCCAATGACTTTCCTTTAC11.7857142857142856No Hit
TATAAGAATAGCTACCCCTAGCTGGGTGTGGTGACTCACACCTGTAATCC11.7857142857142856No Hit
GGCAAAATCGAGTTTGATTGGCCCGACCGGCGATTCCCAGCGTACGCCGA11.7857142857142856No Hit
CTAAAATAAAAGTTTCTGCAAGTACTGAGACAGACAGGTGGGAGGGGCTC11.7857142857142856No Hit
GCTCTAAAACGGTCATATTATGGAGAGGAGAGAGGAATTTTTTTTTTTTT11.7857142857142856No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTGGTTTAAACCACTGCATCCCATG11.7857142857142856No Hit
ATTGACAGGTTTGTTGGATTTCACCGAGGTGAGCGTGGAGCATTATGCTG11.7857142857142856No Hit
TTTCTCATCCTACCTTCAGGTGCCTTCTGCTGAGGCTAAGTCCCAGATGA11.7857142857142856No Hit
CGATGATTCTATTCGATTGCATTCGATAATTCCATTCGATTGCATTCGAT11.7857142857142856No Hit
CTCTTACACGAAATTGTGTGATTTTCTGACTCCAACTGCATGTCCAACAG11.7857142857142856No Hit
AGGTGGTCTACCGCCTGCAGCACGGGGTTGCCGGGCAGCGCTGGGTCAGC11.7857142857142856No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGGATTCAAATCTCGGGTG11.7857142857142856No Hit
TATCTTCAAATAAAAACTAGACAGAAGCATTCTCCTGTCTCTTATACACA11.7857142857142856No Hit
GTGTAAGGTCCGTATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.7857142857142856Clontech Universal Primer Mix Long (95% over 21bp)
ACACGCACTCGTCCTTTCACAGAAGAGGAAATGTCATATCCTGTCTCTTA11.7857142857142856No Hit
ATATAGCTGCTACGGGGCATAGGTACGATGTACGAACGAGGGTTAGAATA11.7857142857142856No Hit
GTCTTGGACATCTGTCGTATTGAAAGCAAGTCACTTCATTTATGTCTGTC11.7857142857142856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)