FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTAAAATTGCTCTGTAACAGAAATGTTCAACTCTGGTAGTTGAATAC35.357142857142857No Hit
CAAAACGCTCCAAATATCCACTTGCAGATACTATGAAAACAGCATTTCAG35.357142857142857No Hit
GGTCAGGAGTTCCAGACCAGCCTGGCCAACATGGCAACTGTCTCTTATAC35.357142857142857No Hit
GATGAAATGTGCTAAGATGCTAGAATTTCCTAATTTTTACAGAAAAGAAG35.357142857142857No Hit
AGCTCAACATGCTGGGTGGCGTGAGTGCGACCGATCAGCCCTGATACGGC23.571428571428571No Hit
ATACAGGGACGAAAAAGGGCACTTTGAGCACTTACGCTGGGCGTTCTTGT23.571428571428571No Hit
GCCACTTAGAAAAAGATATGGAATCAACATATGACTTCAAAGCAACTCGA23.571428571428571No Hit
CGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT23.571428571428571Clontech SMART CDS Primer II A (96% over 26bp)
GCTCTGACCGAATAAGGGATAAAATTTGCCTTAAAACTTGCCTGGCCGGG23.571428571428571No Hit
GAATATCTCTCGGCCTATGCCGCCGCCCTGGGAGGTGAGCAATGAGCGCG23.571428571428571No Hit
GGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGG23.571428571428571No Hit
AATGGACTTCGGCGATTTCATCGAGCGTCGGGGTGCGGCCGGTGCCGATC11.7857142857142856No Hit
GATATGACATTTCCTCTTCTGTGAAAGGACGAGTGCGTGTCTGTCTCTTA11.7857142857142856No Hit
GGGAACTACCCTATTTTAAGGCTTGTTTCTCATTCCTGGCAAAAATGTCT11.7857142857142856No Hit
CAAGAACGTAAATCTCATCTCCGGGCTGCAAAAATGCCGAAGGCGTAATA11.7857142857142856No Hit
GAGAATGCTTCTGTCTAGTTTTTATTTGAAGATACTGTCTCTTATACACA11.7857142857142856No Hit
ACTGCAGACTATACATTTTTTTTCAGCACATGGAATATCCTCCAGAGTAG11.7857142857142856No Hit
TGTGTGGACAGTGGGCTTTTCATGCCTCTCTTACTGTCTCTTACTGTCTG11.7857142857142856No Hit
GGACTGGGAAGGGGAAGCAGGAATCAATTCTTCTCTCATCCCTGTGTCTC11.7857142857142856No Hit
GTATTCCTTTAAGGATATGTTTCCTACATATAGTCGACAATGTTTAAGAT11.7857142857142856No Hit
TGATTACTTCTTGAGCCAGAGTGGAGACAGCAGAAATGGAAAGGAGGTGA11.7857142857142856No Hit
AATGCACACTCACATTTCAGGCTGCAGGAGGGAGAGGGCAGTGGGCTTGC11.7857142857142856No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAA11.7857142857142856Clontech Universal Primer Mix Long (95% over 22bp)
GTGTGAGTCTGTCCTGCCCGGAAAATGCCCTCTGTCCTACTCAGGACACA11.7857142857142856No Hit
ACTCCGTCTCAAAAAAAAAAAAAATTCCTCTCTCCTCTCCATAATATGAC11.7857142857142856No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7857142857142856No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.7857142857142856Clontech Universal Primer Mix Long (96% over 26bp)
CACCTGGCACTATCCTCATTCTACTTGCTGGAAGGTTCAGAGGGAAGCGT11.7857142857142856No Hit
ATCGAATGGTCTCGATTGGAATCATTATCAAATGGAATCGAATGGAATCA11.7857142857142856No Hit
CATAATGCTCCACGCTCACCTCGGTGAAATCCAACAAACCTGTCAATCTG11.7857142857142856No Hit
GCCCTCATGAGTGCCCATACCAACGCCGACGCCGCAGTCGGCGGAGTCAA11.7857142857142856No Hit
AGTGAGATCTCGTGTCGCCACATCCCATGTACTCTGCGTTGATACCACTG11.7857142857142856Clontech SMART CDS Primer II A (95% over 23bp)
GTACCACACCCAGCTAATTTTTGTATTTTTAGTAGAGACAGGGCTTTACC11.7857142857142856No Hit
GATGTAGATCTAGCATATAAGACCTTCTAGAGAAATTTAAAGACATCAGG11.7857142857142856No Hit
AACCTGGGGCGACTCGCAACGCTACTCTATCGACTACTCCAACACTGCCT11.7857142857142856No Hit
CCACTGACCTGTCCTGCCCTGGAATTTGGACAGATGACTGAAGTAGGGGG11.7857142857142856No Hit
AAACGCTGCAACTGGCTTTCACGGCCGGGCGGCATCCACGCCAGCAAGGA11.7857142857142856No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTACCTGTCGG11.7857142857142856No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACAGA11.7857142857142856No Hit
TAGTAAGACAGGGTTTTATTGGGTGAAAAGGAAGAAAAGGGGGAAACAGA11.7857142857142856No Hit
CAATAGTCAGAATGATGCAAACTGAGAAGTCACACCATAAGCTGTCTCTT11.7857142857142856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)