FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTTTATAGACCACTTAGTTGGCGGTGCTGAGGTCTTCCTTCTGTTGG410.81081081081081No Hit
GCGACATGGAGACTAAATTATTTATTTGTTGGATTAACATTGATTTTTCC25.405405405405405No Hit
CCACGATAACAATATGTGGGAAACCACTCCCATGATTTAATTATCTCCCA25.405405405405405No Hit
GAAGAGCGTCGTGTAGGGAAAGGTGAAGGGGCGTTCCCAGTACCTGTCTC25.405405405405405Illumina Single End PCR Primer 1 (95% over 22bp)
GCTCAGTTTATTCAATGGCTCGTTTATTGAATGGAAAAAATATAACCAAT25.405405405405405No Hit
GGATTACATGCCCCTGCCACCAGGCCCAGCTGATTTTTAGAAGAGACAGG25.405405405405405No Hit
GCCGGTAGGCTACCAGGCCGATGCTGACCGCGAGGACGGCGGCGACGAGC25.405405405405405No Hit
CTGTAGTATACAATTTGACTGGTAAAGTTGAGCAGCTTGAAACTCCTCTT12.7027027027027026No Hit
TTCATGAACCCCGGCGGGCTGCGGGCCGACTTCTGCATGGCCTATGGTGT12.7027027027027026No Hit
CCATATTGCAAAGGAGCACGGCGTAAGTGGCGAGGCAAGAAGTAGATGGA12.7027027027027026No Hit
GGGCATGGTGGCAGGCACCTGTAGTCCCAGCTGCTTGGGAGGCTGAGGCA12.7027027027027026No Hit
CTCCCATCCCCACACTCGCTCTAGGAATAAAAGAGCAAAGTATTTTCATG12.7027027027027026No Hit
TCTGAGCACTGGAACTGAAATGCTGTTTTGGCACTTCAGCGCCTGTCTCT12.7027027027027026No Hit
TTATTGGAGGGTAGAGGGTGGTGATGAAATAATCTGTGCAACAAACAACC12.7027027027027026No Hit
CGATGAACTTGTTCAGGTTCGAGATCGTGGTGAACAGATCCTGTCGTCCC12.7027027027027026No Hit
CACATCTCCGAGCCCACGAGACTAGCGTATGTAAATCTCGTATGCCGTGG12.7027027027027026No Hit
GAGGGAACTTAGGAAAATCTTAGAAGCTTTTAAAGCCAGCAAATTACATT12.7027027027027026No Hit
CTGTATTTCCACAATTTGAATGTTGACCTGCTTTGCTAGGTTGGGGAAGT12.7027027027027026No Hit
ACCTCGGCCAGCGCGCGCAGGCGCGAGCTGGAGCCGAAGATCAGGTCGAC12.7027027027027026No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGT12.7027027027027026Clontech Universal Primer Mix Long (96% over 26bp)
GACAAAGCGGCCCTGTCCGCGCAGGCGGAGGAACATGTCGAGGTCCATAA12.7027027027027026No Hit
GAATCAATGCGAGTGGGATGGAATGGAATGAAATGGAATCTCTTTGAATG12.7027027027027026No Hit
TTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGC12.7027027027027026No Hit
GTCCAGGATCACGCAGGAGCGCGTGAGCATGAAGAGCGGCCTGCTTCCGG12.7027027027027026No Hit
GGGCGAGCTGCATGCGGGACATGGGCGCTCCTTCGAGCGGGTCGGTGTCG12.7027027027027026No Hit
ACCTCGGCCAGCGCGCGCAGGCGCGAGTTGGAGCCGAAGATCAGGTCGAC12.7027027027027026No Hit
GCTATCGAGACTTTTTATTTCATCTTGAGAATATTATCTGTCTCTTATAC12.7027027027027026No Hit
CAGAGAAACAGCATGAGGATTCAGTGAGATAGTCCAGGAAAGGGGCTGAG12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)