FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTAACATACATGAAACTACAGGGAACAAAAAACAGGACAGTCACGGT410.81081081081081No Hit
GCCGTGCTCGGTCGCCACGAGCTGCGGTTCACCGACGACGTGGCCCTGAC25.405405405405405No Hit
GCTTATGTGAGTTCAAAAGAATGAAAGCAGTTGTGAAGATGTTAGCAAAT25.405405405405405No Hit
CTGGGCAACAACGTCGGCGGCACCGCCCACGGCGTGCTCACCGACCGTCC25.405405405405405No Hit
ATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCTG25.405405405405405No Hit
AACATTAGGTCTTTATATGGTTTTAACCTTCTGTGGGTCATGAACCTCAT25.405405405405405No Hit
GTACTGGGAACGCCCCTTCACCTTTCCCTACACGACGCTCTTCCTGTCTC25.405405405405405Illumina Single End Sequencing Primer (95% over 24bp)
CTCAGATGAGACTTTGGACTGTGGACTTTTGAGTTAATGCTGAAATGAGT25.405405405405405No Hit
CTCCATTTCATTACGTTACATTACATTACATTGCATTCCACTCTGGTTGA12.7027027027027026No Hit
GCGCTGAAGTGCCAAAACAGCATTTCAGTTCCAGTGCTCAGACTGTCTCT12.7027027027027026No Hit
GGCGATGGGAGCGCCTCGTCATCCCACGCCTCGCCGTCCAGCCCTGCTGC12.7027027027027026No Hit
TCACCAGGCTGACCGCGATGCGTGCCGTCGGGCCGTACGACGAGAGCCTT12.7027027027027026No Hit
CCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG12.7027027027027026No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGT12.7027027027027026Clontech Universal Primer Mix Long (96% over 26bp)
CTACGGCACTCGGTCCTGGAGGGGCGGACAAGAACGGGGCGCTCGACCGG12.7027027027027026No Hit
CCTCATATAAAGAACAAAGACTCATGGAGACACTAGAAAATGGAACCGCC12.7027027027027026No Hit
GTGTAGAGAAGATGTTAAATGACCTAAAGGAGCTGAAAACTGTGGCACAA12.7027027027027026No Hit
GAACCAATGAGTCCAGCTAATTAGTGACACAGATTTATGTCCCAGAACTC12.7027027027027026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.7027027027027026No Hit
CCTTTTTTTTTTTCCTTTTCTTTTAACTTTTATTTCAAGTGTAGGGGTAC12.7027027027027026No Hit
CATCCCTGCCACAGGATGCTTGGAGTCTGCCCTGTCCTAACCACAAAGAC12.7027027027027026No Hit
GTCGGAGACCCCCAGCGCGAGGAACGGCCGGCTGGACCCCTCGGGCTGCC12.7027027027027026No Hit
AATCTCGGCTCACTGCAACCTTCGCCTCCCAGGTTCAAGTGATTCTCCTG12.7027027027027026No Hit
CCTCGGGCTTGACCGGACGGCGAGCCCCGATGCGGCGCGCGCGCCGGCTG12.7027027027027026No Hit
AATAAAAAGTATGCATGATTTTACCACCTGGGAGGCAAAAAACACTTCTT12.7027027027027026No Hit
GAAAACGTTTTTTGTATAAGTATCTTTCTCCCAAAGACCATTTGCTTACT12.7027027027027026No Hit
ATAATATTCTCAAGATGAAATAAAAAGTCTCGATAGCCTGTCTCTTATAC12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)