FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGAATTGAAAATTGGTACCAACCTGTACATCGGTACTACGGGCGAA47.6923076923076925No Hit
CCTGAAGACCTGTGGCCCTAAATGAGGACAGGCATTTCAGGTTTCACACC35.769230769230769No Hit
CCTATGTACCTGCTATGGCCCTAGTCCTGCCCGACACACTAAGTGTGGTT23.8461538461538463No Hit
GTGTGCATTCTACTCCCAAATATGAATCTTCCTTTTCATGAAGCAGTTTT23.8461538461538463No Hit
ACCTCGGCCAGCGCGCGCAGGCGCGAGTTGGAGCCGAAGATCAGGTCGAC23.8461538461538463No Hit
CAGAGAAACAGCATGAGGATTCAGTGAGATAGTCCAGGAAAGGGGCTGAG23.8461538461538463No Hit
GACGTCGTGTAGGGAAAGAGTGTAGTCTGGCCCTACCCACTGTCTCTTAT11.9230769230769231Illumina Single End PCR Primer 1 (95% over 23bp)
GTTTCTTAGAATGACAGGAACTCTTATTCATTGCTCATAAAATAGTACAG11.9230769230769231No Hit
CTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCATGTAGGGAA11.9230769230769231No Hit
TCACCAGTGCGCGAACCAGCTGCACCGTCTTGTACGCGGCGATGCCGACC11.9230769230769231No Hit
GCGGGTGGCGGCTCGCGATAACGTCGCGGGCGACGTCGCGGAGCTTGCGG11.9230769230769231No Hit
GCTGAGTACATAGGTTGATTCCATATCTTGGCTATTGCAGTGCTGCAATA11.9230769230769231No Hit
TTTGTGATGTGTGTCCTCAACTAACAGAGTTGAACCTTGGTTTTGATACA11.9230769230769231No Hit
GCGTCAGACAGCATCTTGATCATCGTGTCGCATAAGTTGAAGACGTTGGC11.9230769230769231No Hit
CCCTCGGCTTCGACGTCGCCGAGCGTCTCGTGGAGCAGTTCGGCGCGGAC11.9230769230769231No Hit
ACATCGTCGCGCAGCTGGCCCAGTATCGGCGCTACGGCTGTCTCTTATAC11.9230769230769231No Hit
GTAGTATGTCAATTGGTGAAAAACAGACTGCGTTTCTAAAGTAGCATAGT11.9230769230769231No Hit
GTGNTGGTGTGCGAGACCACGCTGGCCACGCCGGCGAGGCCGCCGAGCAG11.9230769230769231No Hit
GTAGAATAGCTGGATCAAACAGCTAGGTATGTGATTTAGGTTCTGATGGA11.9230769230769231No Hit
GATGAGTACATAGGTTGATTCCATATCTTGGCTAATGCAGTGCTGCAATA11.9230769230769231No Hit
ACTTGTGACAGGTGTGTCTTGCTTACTTAGCCCACAGCTCTTAACCCCTG11.9230769230769231No Hit
CTCTACCAAGTGCAAGATTCAAATGCAAATTAATGAGAGCTCAGCAGTGA11.9230769230769231No Hit
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACATAAAGGAA11.9230769230769231No Hit
GGTCACCAACATCAAAGATTAAAGGTAGATAAAACCACAAAGAAGGGGAG11.9230769230769231No Hit
TCTCATGACTTACTTAAACATGTAGCTTAGACCCTGTCTCTTATACACAT11.9230769230769231No Hit
AGAACCCGGACGTCGCCGAGCTGGCCCGCGGCAAGGCCGGCCGCCTGATC11.9230769230769231No Hit
TATTATTTCAGTTTAAACGGGAAAATATTTTAAGGCTCAGCAATGCCAGG11.9230769230769231No Hit
GTGCTGTAATCCCAGCACTTTGGGAGGCCTAGGCAGGAGGATCACGAGGT11.9230769230769231No Hit
GGAGAGCACAACTAGGCACATTATTCTGAATATTCTAGGTGCAGAAAAGA11.9230769230769231No Hit
TCACGCCGCTGATGGACATCCCGCAGCCCTTCCAGATCCTCAAGATGGAC11.9230769230769231No Hit
ACAATGGCCTCACCATAAAACACTCTCTAAACCATGGTCCCTGCAATCAC11.9230769230769231No Hit
GCTCAGTTTATTCAATGGCTCGTTTATTGAATGGAAAAAATATAACCAAT11.9230769230769231No Hit
AGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATTGCTTGAATCAGGGA11.9230769230769231No Hit
ACCGAGGGGCTTTATCAACAGTTTGGAGAGGAACGGGTTATTGATACTCC11.9230769230769231No Hit
CACACTAGGACTTTATAAGCTTTCCCTGCCCCTCACACTGAACATCGGGT11.9230769230769231No Hit
CCATTTTATAGACCACTTAGTTGGCGGTGCTGAGGTCTTCCTTCTGTTGG11.9230769230769231No Hit
GACGGGGAGGGAGCAAATTCACACCAGAGCAGCTGTTTCTTCTGCCTGTT11.9230769230769231No Hit
GTGTAGATCTCGTGGTCGCCGTACCCATGTACTCTGCGTTGATACCAGTG11.9230769230769231Clontech SMARTer II A Oligonucleotide (95% over 22bp)
GTCGCAGTAGCGCCGCGCCAGCGCGACGTGGTGCAGGCTGACGATCAGCG11.9230769230769231No Hit
GCCCAAGATGTAGATGTAGAACAGGAATGGTTTCAAATGGTCTTTTCAGA11.9230769230769231No Hit
GGATTACATGCCCCTGCCACCAGGCCCAGCTGATTTTTAGAAGAGACAGG11.9230769230769231No Hit
CCGCCGCCCGAGTCCGACAGTGGCACGGTGTCGGTAAAGGTATTGCCAAA11.9230769230769231No Hit
GGCCTCATCAACGGCGTGCTCAACTACGCCAAGGTCGACGCCGGCGCCGT11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)