FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAAGGTCGGTCGACTCGCGTTACGACAGCGCGATGAATCTTGTCTTC47.6923076923076925No Hit
ACTTTTTATTGAGTGGTGGAGGTGGCTGTCAGCAAGATGGAGTGGGAAGG35.769230769230769No Hit
TACACATTGTTGGTGGAATTGTAAATTAGTACAGCAACTATGGAAGACAA23.8461538461538463No Hit
TCGCTGTATTGCCCAGGCTGGTCTCGAACTCTTGGGCTCAAGCGACCAGC23.8461538461538463No Hit
CTGGGCAACAACGTCGGCGGCACCGCCCACGGCGTGCTCACCGACCGTCC23.8461538461538463No Hit
CATCCCTGCCACAGGATGCTTGGAGTCTGCCCTGTCCTAACCACAAAGAC23.8461538461538463No Hit
CTGCTCTAACAAAAGAAAGATTCAACTCCGTGATTTGAATGCACACATCA23.8461538461538463No Hit
TCTCTACACTGTTTATTCTAGTTAGCCATTCCTCTAACCTTTTCTCAAGG11.9230769230769231No Hit
GGTCTAAGCTACATGTTTAAGTAAGTCATGAGACTGTCTCTTATACACAT11.9230769230769231No Hit
TCGACGCTGTCCGGCGGCCAGCAGCAGCGTGCGGCCATCGCCCGCGTGCT11.9230769230769231No Hit
GTCCACTTCCAGGTAGTGCAGAAAGAGTGTTTCAAACCTGCTCTATGAAA11.9230769230769231No Hit
GGTCTAACATACATGAAACTACAGGGAACAAAAAACAGGACAGTCACGGT11.9230769230769231No Hit
GAGCACCACCTGCCGCACCTTCTCGCGGTCCGCGCGCGCCACGAGCGCGC11.9230769230769231No Hit
CTGCTCAATGAGTCAAACACTGGGACTGATGGTATTAGGTTACAGCTAAT11.9230769230769231No Hit
GATATGGTGTGATGAAAATCTTACTTCTGTGACGAAATTTTACTTCTGTC11.9230769230769231No Hit
CCCTCCAGCTCTGAGAGGCTCCCACTCCCTGGCAAGCACCTCAGACTCCT11.9230769230769231No Hit
CCGTAGCGCCGATACTGGGCCAGCTGCGCGACGATGTCTGTCTCTTATAC11.9230769230769231No Hit
GACACAGCTAAAATAAAACAGAGTGAAGTAATTTTTACAACAGAGGCAAG11.9230769230769231No Hit
GATCTACACTCTTCCCTACATGACGCTCGTTTTTCAGGACCTGTTGGTAC11.9230769230769231No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG11.9230769230769231No Hit
CCAGCGGTCATGGGTACACCACCAGATGCTGCTGTGGGGCTGCACAGAGT11.9230769230769231No Hit
GACCTATGTGTCCTAAGCTTAAATTTATGTTACAGTTCAGGAGTTTCTGA11.9230769230769231No Hit
CGCGGCCGCTGAGCTTCGCGCCGACCGGATACTTGGCGGCGGCGCCGTCC11.9230769230769231No Hit
CGGTTGTACGCCAGCGGGAGCGCCTTGAGCGTTGCCAGCAGACCCGCGTG11.9230769230769231No Hit
ACCCATTTCTCATGCGGAGGTGCCTCCTAAGCTACTTTGCCATTAAATTA11.9230769230769231No Hit
CTGCGGCGCTACTCCCGCAACTCCAACCGCAAGCTCCGCGACGTCGCCCG11.9230769230769231No Hit
TGGGTAGGGCCAGACTACACTCTTTCCCTACACGACGTCCTGTCTCTTAT11.9230769230769231Illumina Single End PCR Primer 1 (95% over 24bp)
ACGTACGGGACTGCGAGGTCGGCGATGCGCGACAGCGTCGAGGACTCGAT11.9230769230769231No Hit
ATTCTACATTCACAGCCTGTTCCATCTACGAGATTTTCCAGATGCTACTT11.9230769230769231No Hit
ATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTGAGCCTG11.9230769230769231No Hit
GGTCGCGCAAGCCTACGACGTCGGGGCTCACGGGCCACCAGGCGCCGAGC11.9230769230769231No Hit
GGTTTTTTACCTCATTAGTGGAAGCAAGTTGTATCACCCTATTTGTTAAT11.9230769230769231No Hit
AACATTAGGTCTTTATATGGTTTTAACCTTCTGTGGGTCATGAACCTCAT11.9230769230769231No Hit
AGGTTGGTATAACCTCTTCAGACAGCCAGCTGGCAGTTTCCATCAGAACC11.9230769230769231No Hit
GTCTACTGCCTTTCCCAAAGTCTGTTGAAAACTCCATCATCGTTAACCAA11.9230769230769231No Hit
GATCTGAACGTCGAGGTGCCGGCCGAGCAACTGGCCGATGCCTGGGGCAA11.9230769230769231No Hit
CAAGCGATCTGCCCATCTTGGCCTGACAAAGTGCTGGGATTACAGGTGTG11.9230769230769231No Hit
GAATATGCTCCCATCGTACGCTGTCGGATTCTTGTGGCATGTGTCATGAT11.9230769230769231No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAGCCTCT11.9230769230769231No Hit
GCACTGGTATCAACGCAGAGTACATGGGTACGGCGACCACGAGATCTACA11.9230769230769231Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GGCTGAGGCAGGAGAAATCACTTGAACCCGGGAGGCAGAGGTTGCAGTGA11.9230769230769231No Hit
GCGCAGCAGTTTCATTAGCATCAAGCAGACCCAGGAGCAGATCGGGGATC11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)