FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCCAGCCCTGGCTGATCAAGACCTTGATCGACGATGGCTTGCTGGCCC24.25531914893617No Hit
TGCCGGGGGTGCGCAGGGTGTACATGGCCGCCAGCAGCAGGGCGAAGCCC24.25531914893617No Hit
CTTCGAGCGAAGATACCTCTCTCTGTTAAAGCCAAGATTTCTTTCTGGAA24.25531914893617No Hit
CTTCAATCCCAACCTCTAACTCAATACATGGTGTTTTCTGTCATCAAGAT24.25531914893617No Hit
ATCCTCCTGAACGAGAAGAACCAGAGTTGCTGCGTGGTTTACTAGCTGGC24.25531914893617No Hit
GGTAGTGGAGCTAGACTTTATGTTGAAGCATTAGATGAAACTAGAGTGAA24.25531914893617No Hit
CACATACCGTGTGGATATGACGCCAGAACAACGACGAGTGCAGGATGCAA12.127659574468085No Hit
CATACATGGGATGTAGGACTGAAAGACTGGAAGCAAGAAGAGTATCAAAG12.127659574468085No Hit
GTTCTGGCGCGCGAAGGTCTGGACTCGTTCGTTTCGCGCGTGGTGTTCTT12.127659574468085No Hit
CCCGTCGATGCATGCCGGAAAGGTCCCGGGGAATCCAGCCGTCGCTATCA12.127659574468085No Hit
CACCCCGATGATATTGTTTTTACGGCAGATGTTATCGATCAGGCGACGCT12.127659574468085No Hit
GGCTAACAGGGTGAAACCCCATCTCTACTAAAAATACAAAAATTAGCTGG12.127659574468085No Hit
GTAACTCACCGATTACTTTCTCACCGAAAGTAGCAACGATATCTGCACCA12.127659574468085No Hit
TTCGTACACCAATTCATCTTCATCATCTGTAGGTTTATATAGCTTGATTA12.127659574468085No Hit
GCGCCGGCGCCGCGCGAAGTACCGGCCGTCGACCTTGTCCAGAACGATCT12.127659574468085No Hit
ATCGGCATCTCCGTGTTTCTGGCGGTCGGTGCCCGCATCTGCCAGGCACG12.127659574468085No Hit
ACGTAGTATTAGCAAAAGGTAAAATCGTAGAGTTAGATGCTCCAGAATTC12.127659574468085No Hit
AACTTGTGTTCAGCGGATTTCTTCAAAATATCAAGCAATTTTTTCTGTTC12.127659574468085No Hit
AAGAAGAACAAAACATTCCTGAACAATTAAATATGTTACATGAGATTCTA12.127659574468085No Hit
TCTCTGGCATACTCGTTACGACAACTTGTTGTGGCGTTGCGAACTGCACA12.127659574468085No Hit
ATCAAGAGGTCGGTTCGACAAGTTGCTATACATATTTGTCATACGGATAA12.127659574468085No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
GGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCAC12.127659574468085No Hit
GAACCAGCGATGACTGCCAGTGGGCTAACATTTCTTCTGTTATGCCAGTA12.127659574468085No Hit
TTGATTGCCTTAATTGCATTCGTAGTCATAACGTCAGCAGATAGAGAGCC12.127659574468085No Hit
CTCCTCACTGAGGTTTGACAAGAATTGAAGAGATTTTGGTAAAAGCTTTG12.127659574468085No Hit
GTTCTCCAACGCGGCCATCGAACTGGCCCTGGCCAGCTACCCCGGGTTCC12.127659574468085No Hit
GATTACTCCTTCTTTATTGGGACCACGTACGACATAAAGATCATCTCTTG12.127659574468085No Hit
ATGTAATCCAGTCCACTACAGGAGTAGCAAGCAAGTTATGATTACGATGC12.127659574468085No Hit
CCCTTGCACTGCACATGGCCCAAGCCGGTCGGGAGGCGCCTCGCATGGAT12.127659574468085No Hit
CCACCGAGCGGTAGAAGCGCTTGAACTCGTTGATCGCGTTCAACTGGAGC12.127659574468085No Hit
ATTTCTCGCATATGTATTAGAAGTGAATTCAATTATAATGATATTGGTGT12.127659574468085No Hit
TCTACACTCTTTCCCTACACGACGCTCTGTCCCAAGTTCTGCAGGTACCA12.127659574468085Illumina Single End PCR Primer 1 (96% over 28bp)
CCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTT12.127659574468085No Hit
ACTCTGACCTTGTTTTCGCAAGCTCTCTTAGGAATTCAATCTTAACTACG12.127659574468085No Hit
CCGCAGCGTCGTGTCGTCTTCATTCTTGACGAGTATGAGACCCTGTTCGG12.127659574468085No Hit
TCCCAAGGTGGTACTGTTCAAAAGAACCTAGAAGTAAGAGGACAGTTCAT12.127659574468085No Hit
GTGATCAGTTCATCATGACAAACCTTGGGCAATTCCGTTTTGGTGATGTA12.127659574468085No Hit
GACTTCATTAGCGGAGAGGGACTAAATCCTTCTCTGCTTTTATTTTAGAG12.127659574468085No Hit
TCACCATTGTTATCATCATCAAATGCAATACGCATAGTTGTACCTTGTGG12.127659574468085No Hit
GCACCGGATTGGCTCGACCTCGAAGGCCATCGCGCACCGGTTTCCCGTTT12.127659574468085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)