FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length146
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAGGTCATCGCGCAAGGCGAACAGGATGCGCCCTGACAAACGCGTAT24.25531914893617No Hit
GTTACATTTAATAGCAGTGATACCCTTTACAGGTTTCTCTGAGTCTTTTA24.25531914893617No Hit
GTCCTCAGCCGTGCAGTCAATCTGGAAGTAGCGATAGTTTGGAGCTGTTA24.25531914893617No Hit
CAAATAACCTGATGAATGGTTACGGTAATGGATACTTTGGAGTTGGCGAC24.25531914893617No Hit
GATTATGAGTTCTCAAAGCATTTAAAGCATCTTGATTTTGTTGAGCTAAA24.25531914893617No Hit
GCCTTCCCTTGCCACTGAGGCCTGACCGCATGTTCACTCGCATCGCACGC24.25531914893617No Hit
GTGAACGTCTTGTCCGGCCTACGGATGGCGCGAGAATTTGTAGGCCTGAT12.127659574468085No Hit
CACCGGCAGAATTTTCCATAGCCTGTGATTGTGCCGTGTCCGTTTTCATT12.127659574468085No Hit
TGTATACACTGATGATATGGCAGCACTAGAAGTAGAGGAAGAGGCAGTTA12.127659574468085No Hit
GCGTGGGCAAAGCGATAAAGTGGTCGCCATCATTGTTACCCGTCTGGATT12.127659574468085No Hit
CCTTCAATGTGATCGAAGAGCGGAAAGTTGTACTGCTTCACCAAGGGGCT12.127659574468085No Hit
GGAAAAATGGGAAAAGGATTACGTAGTTAAGATTGAATTCCTAAGAGAGC12.127659574468085No Hit
ACCGTCAACCGACCTCCGGTACATTACGAGCAGAACCTGAGCGCTCCTAC12.127659574468085No Hit
ACACTAACTGCTGAGCATATTAAGTCGGCTGTAGTAGAAGCGATTAACTT12.127659574468085No Hit
CCTGTAATTTATCTGCTAAGATTTGCATTGGATTAATACCAATCAAATTA12.127659574468085No Hit
GTTCACACCTACTGCACGCTTAGAACCTTTGCCTGCTGTTTTAACAGCTG12.127659574468085No Hit
GTTCCGATGGCTACCGCGTGATGTCGCGTCTATTCCCACCTGCAGGCCAA12.127659574468085No Hit
CAATCTTAGGAGGACTACCGTTATTCGAGAAGCTCGGACCAGCAGGACCA12.127659574468085No Hit
CTATCTAATAGTTCTCGAAGATTGATAGAGGCCTAGCCTGGGGGCTTAAA12.127659574468085No Hit
TACCTGCACTCCGAGCGCTTCGTCGCCGACATGGTCAAGGCGCTCCAGTT12.127659574468085No Hit
ACCAATGTGCATGTGAATGCCGACCAGCTGCAGATGATGACGTTGTATCA12.127659574468085No Hit
GATGTAAAGTATATCTTTATGGATACGAGCCGCAAGGAAACAGATGATAA12.127659574468085No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12.127659574468085Clontech Universal Primer Mix Long (96% over 26bp)
TTTTAAGTTCGATATTTCTCGTTTTTGCTCGTTAACGATAAGTTTACAGC12.127659574468085No Hit
ATCGTTTGGGTGCCTTTTTATTACAAGGTGAAACGGGAAACCGGTGCGCG12.127659574468085No Hit
GAATAAATCTTACCTTTCGCTGTAATCTTATATTTGAAATGAATTTCTGG12.127659574468085No Hit
CCGTGGACGACTCGTTCGTGCCTTCCCGACATTCCAAATGTTTATCATAG12.127659574468085No Hit
TATTCCGTCTATGCAGAATGTGCAGTTCGCAACGCCACAACAAGTTGTCG12.127659574468085No Hit
GTGTTGATGTGTCGGCAACTGTTGTTACTTCAACACTGCCAGCTTCATCT12.127659574468085No Hit
TGTCGGCCGGGGGCGGCACCTCGATGCGCGAGCCGTCGGCGAGCACCGCC12.127659574468085No Hit
CAATCAGATAGTTTCTGCGGCTGACATTGTACCAGTAGATGTTGATTCAA12.127659574468085No Hit
ACTTTGTCTAGCTCTAAAATAAAAGCAGAGAAGGATTTAGTCCCTCTCCG12.127659574468085No Hit
ACCCCGAACTGCTCTTGCTCGACGAACCGGCCGCCGGGCTCGACCTGGGT12.127659574468085No Hit
TCTCAGCTCACTGCAACGTCCGCCTCCTGGGTTCCAGCAGTTCTCCTGCC12.127659574468085No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGTACGGCGAC12.127659574468085Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTAATTCACTTTCTAAATCAAGCATATTTCGTGTGTACCATATCAACTT12.127659574468085No Hit
CGGGCGGCGAGGGTGCCTCATCCGGCGCCGGCCGGGGCGGCGGATCGCGC12.127659574468085No Hit
CATTATACTTACCCTTACCATCATGGCTCTGGATTTCTTGTACAAGTTCC12.127659574468085No Hit
AGCCTGGCCAACATGATGAAACCCTGTCTCTACTAAAAATAGAAAAAATT12.127659574468085No Hit
ACGGTACGTACTTCCACGAACGTATGGAGAAACTGTTTGATGATATGGGG12.127659574468085No Hit
TACTCAGACTTGCGACAGCGTTGTGCTGCCCTGCCGCTCGCGCCTGTGCA12.127659574468085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)