FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGGAACTAGACAGTTTTCCCCCTGCAGAGATGAAAAGCTTTTTGGCT36.8181818181818175No Hit
TCCATCCTCTTCCTCACCATCACCTTCTTCTTCCTCCTCCTCTTCCTCCG24.545454545454546No Hit
CGACACATACACTCTCCCAAGACTAAACCAGGAAGAAGTTGAATCTCTGA24.545454545454546No Hit
GCATCTGCACCTGCAGGCCCGGCTGGTACTGCGCGCTGAGCAAGCAGGAG12.272727272727273No Hit
ATTTTTTTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGACT12.272727272727273No Hit
TGCGCTTGGAAAGAACCATGGCCTGAAGTTGCAGCCCGCCTTTGCGGAAT12.272727272727273No Hit
GCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.272727272727273Clontech Universal Primer Mix Long (96% over 26bp)
CAAGTGACGTCCGCGCCGTCATTTAATTCGTGGTGGAAAAAAGCCCGCCA12.272727272727273No Hit
CTCCCATAGTGCTGGGATTACAGGCATGAGCCACTGTGCCTGGCCTCCAA12.272727272727273No Hit
GACTCATCCTGTTTAGAGATCGGAAGAGCGTCGGTAGGGAAGAGGTGTCC12.272727272727273No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACCCAGTG12.272727272727273No Hit
ATGCAAGAAGCTCTGGCTAAGCTTAAAGAGGAAGAAGAAAGACAGAAGCT12.272727272727273No Hit
GGCCAACACGGTGAAACCCTGTCTCCACTAAAAATAATTAGCCAGGTGTG12.272727272727273No Hit
CCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCT12.272727272727273Illumina Single End PCR Primer 1 (97% over 40bp)
GGTACCAACAGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATGT12.272727272727273No Hit
CGCGTGCCCGGCGATCCCCTGGAGGGCGCACGCATCATGCTCGCCGGGGC12.272727272727273No Hit
TATCAACGCAGAGTACATGGGCGGCGACCACCGAGATCCTGTCTCTTATA12.272727272727273No Hit
TCAAAAAACAACTCAATTCACATATACACAACACCGAGCCCACGAGACCG12.272727272727273No Hit
GCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTC12.272727272727273No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGACTAATAATCTCGTATGC12.272727272727273No Hit
GAGTTACAGTGACCAGCAATTCAAGAGGAGGTTGGATTGGGAGAAGGGTA12.272727272727273No Hit
GCCAAAGACTTGGGAAATATCGCATGCCTTTTGCTTGGACTGCAATCCAT12.272727272727273No Hit
ACCTACAACCCGGTCCTGGACGCCGAGGGCCGGCCGGTGCGCGTGGTCAA12.272727272727273No Hit
TTTCCAGATTCAGCATGGGAAAGATGAGCCATGGGGTAATGACATAAAGG12.272727272727273No Hit
GTTTCCTAACTGCTCTATGAAAAGAAAGGTTAAACTCTGTGAGTTGAACG12.272727272727273No Hit
CAGTGTAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.272727272727273No Hit
GCTTAAGGAGTTTTTGGGCTGAGATTATGGGATTTTCTAAATATACAATA12.272727272727273No Hit
GGTCCGGGCTGGTGGCGCGGGTCCCGCCCGGCCGTGCGAGGGTGGGGTGG12.272727272727273No Hit
TCAAAAAACAACTCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.272727272727273No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA12.272727272727273Clontech Universal Primer Mix Long (95% over 21bp)
TTCCCCTGCTTTCCATTCTTTTGACGCTCTCAGGCACAAATTTTGGCGAC12.272727272727273No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT12.272727272727273Clontech SMART CDS Primer II A (100% over 26bp)
CCACCGAGATCTACACTTTTTTTTTCGTCGGCAGCGTCAGATGTGTAAAA12.272727272727273Illumina Single End PCR Primer 1 (95% over 21bp)
CTTTTTTTTTCGTCGGCAGCGTCAGATGTGTATAAAAAAAAAAAAAAAAA12.272727272727273No Hit
GCAGAAGACGGCATACGGGATCTTGCTTCGTGACTGGAGTTCAGACGCTG12.272727272727273RNA PCR Primer, Index 28 (95% over 24bp)
AAAGAAGAGTTACAGAAAAAAGAAGCCGGCCGGGCGCGGGGGATCACGCC12.272727272727273No Hit
GGGCGTGCGCACAGGAACACCGCCCCCACGATGAGCAGCGATGCCACCGC12.272727272727273No Hit
CTACATGGGCACTTTCCACGACGCTCTTCCGATCTCATGATGCAAGGACT12.272727272727273Illumina Single End PCR Primer 1 (95% over 21bp)
GCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGAGTTCAGACGTGT12.272727272727273Illumina PCR Primer Index 1 (96% over 25bp)
GGCCCACAACAGGCGCTTGTGGTCGGACGAATGGTCGATGCCTCCAAGCT12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)