FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGGTTTTCCTTTCACTCTTGTAGTAGAATGCTGATCTAACGGGAGT36.8181818181818175No Hit
GGCTTTTTCTGCATCTATTGAGATAATCATGTGGTTTTTGTCTTTGGCTC24.545454545454546No Hit
GTCTAGTCATCCTCGTCGGTCTTCTGCTCTGAAAAGCCATCTTTGCATTG24.545454545454546No Hit
GCACTCCAGCCTAGGTGACAGACTAGAGACTCTGTCTCAAAAACAAAAAA12.272727272727273No Hit
ATTAAATGGATTGCAGTCCAAGCAAAAGGCATGCGATATTTCCCAAGTCT12.272727272727273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAC12.272727272727273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12.272727272727273No Hit
CTTCCCATGTACTCTGCGTTCATGGGAAGCAGTGGTATCAACGCAGAGTA12.272727272727273Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CTGCAAAGTTCTCTCACCACCCGAGGGACGACATCCACGAGATGGTGGGG12.272727272727273No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATAACCTGTC12.272727272727273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG12.272727272727273No Hit
AATCTCTTCCGATTGAAGATTTTCCCGCAAAACTCGCACGTCTTCTGGGG12.272727272727273No Hit
CGCCGGCATCGAGCAGCTTCGTGGCATAGAGATTCATCGTGTCGGCGTGA12.272727272727273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAT12.272727272727273No Hit
GGGTCGCATAAGATCCGGTGGTGCTTGCCGGTGATCTCCTCGAGTTCGTA12.272727272727273No Hit
AGGAGGACGATTCGTCGGTGCCTATGAAAGATGGACCCTTTGCCTACGGT12.272727272727273No Hit
GATCTCGGTGGTCGCCGCCCCTGTAATCTGCGTTGATACTGTCTCTTATA12.272727272727273No Hit
CATTTTAACCCTTTATGTCATTACCCCATGGCTCATCTTTCCCATGCTGA12.272727272727273No Hit
CAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACAATGCTTTT12.272727272727273No Hit
CTCCGGGGACAGTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTC12.272727272727273No Hit
CCCATATATGGAAAAAAGAATATATCTAAGTGACTGCCTGCTCTAAAGGA12.272727272727273No Hit
CCGGACACCTCTTCCCTACCGACGCTCTTCCGATCTCTAAACAGGATGAG12.272727272727273No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG12.272727272727273No Hit
TACGTGTAGCGCAGCTTGGCATTGGGTTTCACGTTTTCGGGCAACCCGCG12.272727272727273No Hit
CATCCCTTCATGTTAAAATACACTAGGTGTTGATGGAACATATCTCAAAA12.272727272727273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.272727272727273No Hit
CGTCTGAACTCCAGTCACGAAGCAAGATCCCGTATGCCGTCTTCTGCCTG12.272727272727273TruSeq Adapter, Index 9 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGACC12.272727272727273No Hit
GGGTTCAATGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA12.272727272727273No Hit
AAAAAAATCTGTCTCTTATACACATCTGACGCTGCCGACGATTACCATAA12.272727272727273No Hit
GACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGACTGTCTCTTAT12.272727272727273Illumina Single End PCR Primer 1 (96% over 25bp)
CTTCTGTCTTTCTTCTTCCTCTTTAAGCTTAGCCAGAGCTTCTTGCATCT12.272727272727273No Hit
GGCGCGAGGGCAGGGGGCAACCGGACCCCGCCCGCACCCATGGCGCCCGT12.272727272727273No Hit
GCTTTGAGGTCCATGGTAGAAAAGGAAATATCTTCGTATAAAAACTAGAC12.272727272727273No Hit
GATACGACCTGCGGATCGGTAATGGCGGCTATGATGTCGCCAAAATTTGT12.272727272727273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGGGAAGAGCAAAC12.272727272727273No Hit
GTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTAGTGTGATCCTGGCTCAC12.272727272727273No Hit
GTTCAAGAGACTCTCCTGCCTCAGCCTCCCAAGGAGCTGGGACTACAGGC12.272727272727273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCCAGGGGA12.272727272727273No Hit
CACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTTTGCTCCCAGTGG12.272727272727273No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)