FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTACTTTTTTTTTCGTCGGCAGCGTCAGACTGTCTCTTATACACATC25.128205128205128No Hit
CTTCAGGGAGAGGAAGCCGGCGGCCTCACTGCTATGGGCCGCAACAAGAA25.128205128205128No Hit
CGCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCA25.128205128205128No Hit
CTCCTCGAAATCCACAGTCATCTTCATCCTGCCTCCGCCTCGCAGGCGGC25.128205128205128No Hit
TGGATATTCTGACATCTTGTGGCCTTCGTTGGAAACGGGATTTCTTCATA25.128205128205128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGG12.564102564102564No Hit
CAAAAAATCATTACAACACTAAGCACATCAAAAATAAAAAAATAAACATC12.564102564102564No Hit
CGACCACCGAGATCTATACTTTTTTTTCGTCGGCAGCGTCAGATGTGCTG12.564102564102564No Hit
ACCTGAGCTGTTGCATTGTGGCGAGCTCTCTTAAGTCTGTCTCTTATACA12.564102564102564No Hit
GTATAACACTCCCCTTCCTAGGAAACCATTTGAATGGGTGTCTTTTTATT12.564102564102564No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12.564102564102564No Hit
GGGGTACAAAGCGGTTTTATTCGGTATAATACGGGATCGTTGGGTTCGAG12.564102564102564No Hit
CGCGTGCCCGGCGATCCCCTGGAGGGCGCACGCATCATGCTCGCCGGGGC12.564102564102564No Hit
CAAAAAAACAACACTCATCAAATCACAAAAAACTATCACTAATAAACAAC12.564102564102564No Hit
CAGTGTAGCGGGGGGGGGGGGGGGGCGGTGGGGGGGGGGCGGGGGGGGGG12.564102564102564No Hit
CAGGTAGATTGACCAAACCACAGCATAATGACCCACTGTGAGAATAATGA12.564102564102564No Hit
GTGCTGCTCTGTAGGCTGGCAGAGGACAGTGGAGCAGCACTAAAAGCACT12.564102564102564No Hit
GATCATAGGCGCCGGCCACGAGATTGGCGGCGTGAAACGGCGTCTGGTCG12.564102564102564No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGACTAATAATCGCGTATGCC12.564102564102564No Hit
GTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCGCATCTCCGCTCACT12.564102564102564No Hit
CTTTTTTTTCGTCGGCAGCGTCAGATGTGAAAAAAAAAAAAAAAAAAAAA12.564102564102564No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC12.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
GCTATTAGGTATCACTGCTTCTGAATCTACTCAATGAAAAGATCTAGAGA12.564102564102564No Hit
GGCCAAGATGGCCGATTAGAAGCAGCTGCAGCTGGGGGCTACCACCCAGA12.564102564102564No Hit
TTCCTTTCGATTGCATTCGATTATGATTCATTTCGTGTTCATTCTATGGT12.564102564102564No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAG12.564102564102564Clontech Universal Primer Mix Long (95% over 21bp)
CGATGATTCTATTCGATTCCATTCAATGATAATTCCATTCAATTCAGTGA12.564102564102564No Hit
GTATTTATTGGACCCAGAGAACCGGGCCGGGCACAGCGTGGCTACAGAGG12.564102564102564No Hit
TCTACACTCTTTCCCTACACGACGCTCTTCCGAAAAAAAAAAAAAAAAAA12.564102564102564Illumina Single End PCR Primer 1 (96% over 33bp)
TTCTGGTAGGGTCCGTTCGATGTTGATTCCATTGGAGTGCATTCGATGAT12.564102564102564No Hit
ACGCAAGGGTGGCCTAGAGTTATGAGACTTCCTTTTCCACCCAGTCCTGA12.564102564102564No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT12.564102564102564Clontech SMARTer II A Oligonucleotide (95% over 21bp)
ATTTTGTATCCTTACTATTCATGACTGTTGGAATAAAATAGAAATCAAAA12.564102564102564No Hit
CTTTGGCGATTTACCAGCACCTTGTTGAAGAACTTGATACTGTAAACCTG12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)