FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCTATGACCAGCGGGCGAGGCGCCCTTCGCAGCTCCGCGCAACGCTAG25.128205128205128No Hit
GTACATGGGCATACCGGACTCATCCTGTTTAGAGATCGGAAGAGCGTCGT25.128205128205128No Hit
GCAGCTGAAATCTCCACTTGCAAATTCCACAAAAAGAGTGTTTCCAATCT25.128205128205128No Hit
CTGCCAGCATCTTCAAGTATTCCTGGTGGCGCTCCTCCCGAAGCTTCCTC25.128205128205128No Hit
TCTGACGCTGCCGACGAAAAAAAAAGTAGATCCTGTCTCTTATACACATC25.128205128205128No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG25.128205128205128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA12.564102564102564No Hit
AGCAGAAATAGGCCAGGTGCGGTGGCTCACGACTGTAATCCCAGAATTTT12.564102564102564No Hit
ATCGGCGGCGAGTCGGCGGGCGCCCATCTCTCGGTGCTGGTCCTCCTGCG12.564102564102564No Hit
CTTTCCCTACACGACGCTCTTCCGATCTGGTACCAACAGGTCCTGAAAAA12.564102564102564Illumina Single End PCR Primer 1 (100% over 28bp)
ATTCAGCCGAGTGGCTGTTGAGAATTTGCGCAGCTCTGTGGTTGGGGCCC12.564102564102564No Hit
CGCCGGCATCGAGCAGCTTCGTGGCATAGAGATTCATCGTGTCGGCGTGA12.564102564102564No Hit
GATCTTCGCCGCCTTCATCAGGGGTGATGAAGCCGAAGCCCTTCGAGTCA12.564102564102564No Hit
GCCGTAGCCTCTGGTGGTGGCTAGTGTGGAACCACTGCCCTACAAGCTGG12.564102564102564No Hit
GAATGGAATCGAATGGAATCATCAAATGGAATCCAATGGAATCATCATCA12.564102564102564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA12.564102564102564No Hit
ATTGAGATGGTTTCCCACTCATCTTGACTCAGAGTGCTTTTAGTGCTGCT12.564102564102564No Hit
GGCTGGTCTTGAACTCCTGACCTCAGGTGATCTGCCCATTTTGGCCTCCC12.564102564102564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAA12.564102564102564No Hit
ACTTAAGAGAGCTCGCCACAATGCAACAGCTCAGGTCTGTCTCTTATACA12.564102564102564No Hit
GTCTGAGCTGCTGCGACCCGAGCTGGATGCGCCGGGCGCGGCCCCCTCCC12.564102564102564No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGCGTTTATACCACAGCTTACCA12.564102564102564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.564102564102564No Hit
GAATCATTGAATGGAATTGAGTGGAATCACCAATGAATGGAATCGAATGG12.564102564102564No Hit
GGGTTCAATGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA12.564102564102564No Hit
CACATCTGACGCTGCCGACGAAAAAAAAGTATAGATCTCGGTGGTCGCTG12.564102564102564Illumina Single End PCR Primer 1 (95% over 21bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.564102564102564Clontech Universal Primer Mix Long (96% over 26bp)
ATCGAATGGAATTGAATGGAAACATCAATTGGACTCGAATGGAAACATCA12.564102564102564No Hit
CTTTACAAACTGTTTTAATCCCTTCAGTCAATTTAAAATGCATGCACCCA12.564102564102564No Hit
GTAGAAAGTGTGATGCCTGGTTTTGATTTCTATTTTATTCCAACAGTCAT12.564102564102564No Hit
CTCTCAGTGATGAGCAAATGGCGACTGTTCTCAACCAATATAAAAAGCGA12.564102564102564No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTAAA12.564102564102564No Hit
GTACATGGGTGAGAAAAATGAGCAATGCTGAGCTGGCATTACTCTTGTTC12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)