FastQCFastQC Report
Tue 7 May 2024
EGAF00003662261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070
Total Bases448.2 kbp
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTG220.7166123778501629No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT120.39087947882736157No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC120.39087947882736157No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA110.3583061889250814No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA100.32573289902280134No Hit
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG100.32573289902280134No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT100.32573289902280134No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC90.2931596091205212No Hit
GGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGCACTCTTT80.26058631921824105No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC80.26058631921824105No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA80.26058631921824105Illumina PCR Primer Index 1 (96% over 28bp)
GAATATAGCAAACGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTT80.26058631921824105No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT70.2280130293159609No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG70.2280130293159609No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT70.2280130293159609No Hit
GCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC60.19543973941368079No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT60.19543973941368079No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT60.19543973941368079No Hit
GTGCAGGAGTCACCGGCGGCGCAGGAGCAGTTGGGATCCATGGCGAGCTG60.19543973941368079No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA60.19543973941368079No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT60.19543973941368079No Hit
ACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGC60.19543973941368079No Hit
CCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTT50.16286644951140067No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG50.16286644951140067No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG50.16286644951140067No Hit
CTGCTGCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAG50.16286644951140067No Hit
CTGCAAAGGGGCGTCGGACAAGTGCAGCTGCTGCGCCTGATGCTGGGACA50.16286644951140067No Hit
GCAAATGGCTCAGTATCGTATTGAAAAAAAATCCAGGTTGTGCAGCTTGT50.16286644951140067No Hit
CACTTGGCACAGCCCACGGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGA50.16286644951140067No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA50.16286644951140067No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT50.16286644951140067No Hit
TTGCAGACACAGCCGTGGGCACACTTGGCACAGCCCACGGGGCAGCAGGA50.16286644951140067No Hit
CATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAG50.16286644951140067No Hit
GAAATGCAGCAAATGGCTCAGTGTTGTATTTTTAAAAAAATCCAGGTTGT40.13029315960912052No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC40.13029315960912052No Hit
GCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAA40.13029315960912052No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT40.13029315960912052No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG40.13029315960912052No Hit
CCCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGC40.13029315960912052No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC40.13029315960912052No Hit
CTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCT40.13029315960912052No Hit
GTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC40.13029315960912052No Hit
TCTCCAACGTCCCTTTGCAGACACAGCCGTGGGCACACTTGGCACAGCCC40.13029315960912052No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT40.13029315960912052No Hit
CAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCG40.13029315960912052No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC40.13029315960912052No Hit
GCTCCTGCAAGTGCAAAGAGTGCAAATGCACCTCCTGCAAGAAGAGCTGC40.13029315960912052No Hit
CGCCATAATGGCACTGGTGGCAAGTCCATCTATGGGGAGAAATTTGAAGA40.13029315960912052No Hit
CTTACACACTTCCTAATGGCAGAATTTGGCTGTTTGGCTTCAACTCCTAC40.13029315960912052No Hit
CCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCA40.13029315960912052No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC40.13029315960912052No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT40.13029315960912052No Hit
AATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGC40.13029315960912052No Hit
CGGCTGTGTCTGCAAAGGGACGTTGGAGAACTGCAGCTGCTGTGCCTGAT40.13029315960912052No Hit
CGTCCCACCCCACTTCTTCCACCCCACTTCTTCCTTCACCAACATGCAAG40.13029315960912052No Hit
CGGTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCG40.13029315960912052No Hit
ACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGT40.13029315960912052Illumina PCR Primer Index 1 (96% over 28bp)
GCCCAGGTCAGGGCATCTGGAGTCTGAAGGACCCTAGTTCCTAGAGGCAT40.13029315960912052No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCGAACCCGCGTGCAACCTGTC40.13029315960912052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.13029315960912052No Hit
CCTTATAGCCTCTCAACTTCTTGCTTGGGATCTCCAACCTCACCGCGGCT40.13029315960912052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)