FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3070
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTG260.8469055374592834No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA250.8143322475570033No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT240.7817589576547231No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC220.7166123778501629No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT150.4885993485342019No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT130.4234527687296417No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT120.39087947882736157No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA120.39087947882736157No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT110.3583061889250814No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC110.3583061889250814No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC100.32573289902280134No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC100.32573289902280134No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA100.32573289902280134No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT90.2931596091205212No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA90.2931596091205212No Hit
GTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGG90.2931596091205212No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT80.26058631921824105No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT80.26058631921824105No Hit
CTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT70.2280130293159609No Hit
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTGCC70.2280130293159609Illumina PCR Primer Index 1 (96% over 28bp)
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG70.2280130293159609No Hit
ACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCA70.2280130293159609No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT70.2280130293159609No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCGAACCCGCGTGCAACCTGTC70.2280130293159609No Hit
GCCCACGGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGAGGTGCATTTGC60.19543973941368079No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC60.19543973941368079No Hit
CCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTT60.19543973941368079No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC60.19543973941368079No Hit
AAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGT60.19543973941368079No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC60.19543973941368079No Hit
GCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCG60.19543973941368079No Hit
CGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAA60.19543973941368079No Hit
TAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGG60.19543973941368079No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.19543973941368079No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGT50.16286644951140067TruSeq Adapter, Index 7 (96% over 30bp)
GGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGAGGTGCATTTGCACTCTTT50.16286644951140067No Hit
ATTAACATCTGGGAGAAGAGCTGTTCCCACATCAGGCACAGCAGCTGCAG50.16286644951140067No Hit
ACGCAGAGTACATGGGAGGCCCGTGGCGCCGACAGGATGGGCAAGTGTCG50.16286644951140067No Hit
CACTTGGCACAGCCCACGGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGA50.16286644951140067No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT50.16286644951140067No Hit
CACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCA50.16286644951140067No Hit
GCCAGAGGGTGTACTTGAGGAAATTGAAAATGTAGAAGAGGCTGATCTGA50.16286644951140067No Hit
GACCCCAACTGCTCCTGCACCACTGGTGTCTCCTGCGCCTGCACCGGCTC40.13029315960912052No Hit
GGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGCACTCTTT40.13029315960912052No Hit
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG40.13029315960912052Illumina PCR Primer Index 1 (96% over 28bp)
GCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGC40.13029315960912052No Hit
ACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGT40.13029315960912052No Hit
GTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGCAGCTGCT40.13029315960912052No Hit
CTCCACAATATTCATGCCTTCTTTCACTTTGCCAAACACCACATGCTTGC40.13029315960912052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.13029315960912052No Hit
CAACGCAGAGTACATGGGGCAGTGGTATCAACGCTGTCTCTTATACACAT40.13029315960912052No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC40.13029315960912052No Hit
GACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGAC40.13029315960912052No Hit
ATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGG40.13029315960912052No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT40.13029315960912052No Hit
AGTCAAAGCTGTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATA40.13029315960912052No Hit
CCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTC40.13029315960912052No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT40.13029315960912052No Hit
AACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTG40.13029315960912052No Hit
CCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCC40.13029315960912052No Hit
GTACGTTGCTCAGCTCCTCCCGCTTCCTCTTGGCGCGGATGTGCGTCCCC40.13029315960912052No Hit
GTACATGGGACGCCTCCTCCGAGTCCCAGCGAACCCGCGTGCAACCTGTC40.13029315960912052No Hit
CTCCTGCACCACTGGTGTCTCCTGCGCCTGCACCGGCTCCTGCAAGTGCA40.13029315960912052No Hit
GCACTTCTCTGATGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC40.13029315960912052No Hit
GCTCGAAATGGACCCCAACTGCTCTTGCGCCACTGGTGGCTCCTGCACGT40.13029315960912052No Hit
GAAAATAGAAAGACGACCTGGGTTCTCTGGGTAGGCGCAATGTGATCATA40.13029315960912052No Hit
TCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCT40.13029315960912052No Hit
TTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTAC40.13029315960912052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA40.13029315960912052No Hit
ACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGC40.13029315960912052No Hit
ATGCAAAGAGGTTGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACAT40.13029315960912052No Hit
GTTCTATTAACATCTGGGAGAAGAGCTGTTCCCACATCAGGCACAGCAGC40.13029315960912052No Hit
GTCTGGTCCCTGTTCAACACCCTCTTCATGAACACCTGCTGCCTGGGCTT40.13029315960912052No Hit
GGGAAGGAAAGAACTTGCATGTTGGCGGCGCACGCCTCATTACGATTCGC40.13029315960912052No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGAGGTGCATTTGC40.13029315960912052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)