FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC210.6923837784371909No Hit
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTG200.6594131223211342No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA170.5605011539729641No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA160.5275304978569073No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT150.4945598417408506No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT110.3626772172766238No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC100.3297065611605671No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC100.3297065611605671No Hit
GCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTT100.3297065611605671No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT90.2967359050445104No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT80.26376524892845365No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA80.26376524892845365No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA80.26376524892845365No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT80.26376524892845365No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT70.23079459281239698No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC70.23079459281239698No Hit
CTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGATCGGGAACTCCTGCT70.23079459281239698No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT70.23079459281239698No Hit
GTATCGTATTGAAAAAAAATCCAGGTTGTGCAGCTTGTTCTATTAACATC70.23079459281239698No Hit
AAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGT60.19782393669634024No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCC60.19782393669634024No Hit
CTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTG60.19782393669634024No Hit
GCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGC60.19782393669634024No Hit
GGGCTACACCTTGACCTAACGTCTTTACGTGGGTACTTGCGCTTACTTTG60.19782393669634024No Hit
ACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGT60.19782393669634024Illumina PCR Primer Index 1 (96% over 28bp)
GGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGCACTCTTT50.16485328058028356No Hit
CTGCACCACTGGTGTCTCCTGCGCCTGCACCGGCTCCTGCAAGTGCAAAG50.16485328058028356No Hit
CCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTT50.16485328058028356No Hit
TCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCG50.16485328058028356No Hit
TTCCTAGAGGCATCTGGCAGCAAGAAGGTGAGGCATCAGGGAACGGGAAT50.16485328058028356No Hit
GTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC50.16485328058028356No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG50.16485328058028356No Hit
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGTAGCTTTTCTTG50.16485328058028356No Hit
CACTTGGCACAGCCCACGGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGA50.16485328058028356No Hit
CAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTT50.16485328058028356No Hit
CCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAG50.16485328058028356No Hit
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTG50.16485328058028356Illumina PCR Primer Index 1 (96% over 28bp)
TGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTG50.16485328058028356No Hit
CCCAGTGACTGCAGAGTAGCCACAAGGCTGCCCGAGGCAACTCCACCCCC40.13188262446422683No Hit
GTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAG40.13188262446422683No Hit
GCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGG40.13188262446422683No Hit
CAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGGGCT40.13188262446422683No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40.13188262446422683No Hit
CAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCCGTGGGCT40.13188262446422683No Hit
TCCTTGCCTCGAAATGGACCCCAACTGCTCCTGCTCGCCTGTTGGCTCCT40.13188262446422683No Hit
CCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTC40.13188262446422683No Hit
GTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGG40.13188262446422683No Hit
GTTGGTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAA40.13188262446422683No Hit
GGTCAGGGTTGTATGGAAAAAAAATTCCAAGTTTGTGCAGGTCACTCTTT40.13188262446422683No Hit
TGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAG40.13188262446422683No Hit
CAATGAGGGGGAGGAAGGGCCACATCTCCCTCTGCAGGCCCTTTGACCAT40.13188262446422683No Hit
ATTCCCTGTCTGTGTGCAGAGGGGATTCAACTTCAATTTTTCTGCAGTGG40.13188262446422683No Hit
ACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCG40.13188262446422683No Hit
AAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCA40.13188262446422683No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC40.13188262446422683No Hit
GTCCGGACATCAGGCACAGCAGCTGCACTTCTCTGATGCCCCTTTGCAGA40.13188262446422683No Hit
CCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCC40.13188262446422683No Hit
CTCCATTTATCGCTTGAGATCTCCAGCCTTACCGCGGCTCGAAATGGACC40.13188262446422683No Hit
GCTCTTCTCCCAGATGTAAATAGAACAACCTGCACAACCTGGATTTTTTT40.13188262446422683No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTACACTTGTCCGACGCC40.13188262446422683No Hit
CGGTTAGACCAGATTCCTGCCGCCAACCTTGATGCAGATGACCCTCTAAC40.13188262446422683No Hit
GGTCCCGGCAGGCACAGGGCTCACACCTTGGGTTTTGCAAACACCTCCCA40.13188262446422683No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT40.13188262446422683No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCGAACCCGCGTGCAACCTGTC40.13188262446422683No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)