FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC210.6923837784371909No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA200.6594131223211342No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA160.5275304978569073No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT160.5275304978569073No Hit
GGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTG140.46158918562479395No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC140.46158918562479395No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC120.3956478733926805No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC120.3956478733926805No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT120.3956478733926805No Hit
GTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT100.3297065611605671No Hit
ATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCC100.3297065611605671No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT90.2967359050445104No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA90.2967359050445104No Hit
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG80.26376524892845365Illumina PCR Primer Index 1 (96% over 28bp)
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC80.26376524892845365No Hit
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTGCC80.26376524892845365Illumina PCR Primer Index 1 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.23079459281239698No Hit
GTTCTCCAACGTCCCTTTGCAGACACAGCCGTGGGCACACTTGGCACAGC70.23079459281239698No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT70.23079459281239698No Hit
TGCTGGGACAGCCCCGCTCCCAGATGTAAAGAACGCGACTTCCACAAACC70.23079459281239698No Hit
AATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGC70.23079459281239698No Hit
GCCCACGGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGAGGTGCATTTGC60.19782393669634024No Hit
ATTTACATCTGAGAGCACAGCTGTCCTGGCATCAGGCACAGCAGCTGCAC60.19782393669634024No Hit
CCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTT60.19782393669634024No Hit
GAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTT60.19782393669634024No Hit
ATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGG60.19782393669634024No Hit
GATGTAAAGAACGCGACTTCCACAAACCTGGATTTTTTATGTACAACCCT60.19782393669634024No Hit
GAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCC60.19782393669634024No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAG60.19782393669634024No Hit
TATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACT60.19782393669634024No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT60.19782393669634024No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA60.19782393669634024No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGAGGTGCATTTGC60.19782393669634024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.19782393669634024No Hit
GACCCCAACTGCTCCTGCACCACTGGTGTCTCCTGCGCCTGCACCGGCTC50.16485328058028356No Hit
GGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGCACTCTTT50.16485328058028356No Hit
GCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCTGCAAGAAA50.16485328058028356No Hit
CAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGTGGGCT50.16485328058028356No Hit
ATATGGTTTACATTCTATCTCCAGAGAAAACACACTTAACAGAAGACAGA50.16485328058028356No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC50.16485328058028356No Hit
ATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGTACAT50.16485328058028356No Hit
GCTCGTCCTGATCCTGTGATTTCTCTGTCCTTGGCTATTCTTGCAGGGAG50.16485328058028356No Hit
GAGTGCAAATGCACCTCCTGCAAGAAAAGCTGCTGCTCCTGCTGCCCTGT50.16485328058028356No Hit
GTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAC50.16485328058028356No Hit
GTATCAACGCAGAGTACATGGGGACCACGCCTCCTCCAAGTCCCAGCGAA50.16485328058028356No Hit
GCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAA50.16485328058028356No Hit
CTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCTCCT50.16485328058028356No Hit
GTTTTATTGTCACTCACATATTTAACATAAAAAGAAATGCAGCAAATGGC50.16485328058028356No Hit
AACTAACACAAAGGAAGTCTGGGCAATGTTGCGAGACCCCGTCTCTGGAA40.13188262446422683No Hit
GCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC40.13188262446422683No Hit
TCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCG40.13188262446422683No Hit
CTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGC40.13188262446422683No Hit
TCTTTTGTCTTTCTTCTGGTCATCTACACCTTTTGCACAGTTCTTGAAGA40.13188262446422683No Hit
GGTTTATATAGGTTGCTCTATTTACATCTGAGAGCACAGCTGTCCTGGCA40.13188262446422683No Hit
GCTGTCCTGGCATCAGGCACAGCAGCTGCACTTGTCTGACGTCCCTTTGC40.13188262446422683No Hit
ACGCAGAGTACATGGGGAGGCTCCAACGTGCCTTCCAGCTGCCTGACTGC40.13188262446422683No Hit
AAACTGGGGATTGCAGTGTTGTCAGGTGCTACCGTTTCAGCAGTTGGTTT40.13188262446422683No Hit
AAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGACGCCTCCTCC40.13188262446422683No Hit
GCAGCTGCAGTTCTCCAACGTCCCTTTGCAGACACAGCCGTGGGCACACT40.13188262446422683No Hit
GTCCTGGCATCAGGCACAGCAGCTGCACTTGTCTGACGTCCCTTTGCAGA40.13188262446422683No Hit
CTACAGCACCACCAGTATTGCCAGCGTCGCTGGCCTGACCGCCGCTGCCT40.13188262446422683No Hit
TAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCTGGGACCACGC40.13188262446422683No Hit
GTACATGGGACCACGCTTTTCATCTGTCCCGCTGCGTGTTTTCCTCTTGA40.13188262446422683No Hit
GACCTCCGTCTATAAATAGAGCAGCCAGTTGCAGGGCTCCATTCTGCTTT40.13188262446422683No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT40.13188262446422683No Hit
GCACTTCTCTGATGCCCCTTTGCAGATGCAGCCCTGGGCACACTTGGCAC40.13188262446422683No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA40.13188262446422683No Hit
CTCCAGGGCCACGGGGCCTGGGCAGGAGGCTGCCCTTCAAGGAGGGTATC40.13188262446422683No Hit
ATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGT40.13188262446422683No Hit
CGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCG40.13188262446422683No Hit
TCCCACATCAGGCACAGCAGCTGCAGTTCTCCAACGTCCCTTTGCAGACA40.13188262446422683No Hit
TTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGA40.13188262446422683No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCAGGGTCAGGGTTGT40.13188262446422683No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT40.13188262446422683No Hit
CAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGCCCTG40.13188262446422683No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)