FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length146
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCTGAGGCCTCACCAGAAGCAGATGGTGACATGATGCTTTGTGTACA25.714285714285714No Hit
GGCAGAAGGGATTTCTATCAAAGTGGTAACCATGGTTAAAAATGACTTGT25.714285714285714No Hit
GTATTCGGGGTGTGTGGTAGGAATTGGAAAAATAATTGTTGAATCTAAAT25.714285714285714No Hit
GCAGATACCGAGCACTGGCAGGCCCCCATCGACAGCTCTGCGAATAGACG25.714285714285714No Hit
GGGGAGAAGAGGGTCTGCCCACTGTGGGCCTGCGGGTCATAGCTGGCCGG25.714285714285714No Hit
CCACGGTGAAACCCCATCTCTACTAAAAATACAAAAAATTAGCTGGGTGT25.714285714285714No Hit
GTCCAGGATCCAATGCAGAGTTACACTGCATTTACAATCTGGAAGAATTC12.857142857142857No Hit
CCATCACGCCCTCTTCAGAGACATCTTCATCATCCTCCAAGAAACAAGCA12.857142857142857No Hit
AGACATGTCTTTCCAATTATTCTGATCTATTTTCCTGTCATTTTCCCCAT12.857142857142857No Hit
ATGCCACCGGCCGCGACACGCTGCTGGCCAACAAATTCCGCTGCAAGCAG12.857142857142857No Hit
TACTTGCATAGGAGTCACATCAGGATGCGTATGTACGTCGAGCAGCGCCG12.857142857142857No Hit
TCAGAACATTTTCTCTTGGAAATTTGCTGACAGGCTCAAATTCGAACCAG12.857142857142857No Hit
CAATATTACAGCCGCAACTACTGACATGACGGGTGATGGTGTTCACAATT12.857142857142857No Hit
CTCTTTTCTTCTTTATTAGTCTTGCTAGCGGTCTTTCAATTTTGTTGATC12.857142857142857No Hit
GGGGAGAGGTATGCCTCCTGGGCGGCCGCGGCCACGTGCGGGCCGAGCAC12.857142857142857No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGGTATCAACTG12.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTCCATTCGATGATTCCATTTGATTCCATATGATGATGATTCCATTC12.857142857142857No Hit
GCCTCCAAGATTGAGATCCGCAAGGAGGGCAAGATCGGCCGCTGTCTCTT12.857142857142857No Hit
CCCATGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGA12.857142857142857Clontech SMART CDS Primer II A (96% over 25bp)
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.857142857142857No Hit
GGCCTGGGCCTGTCGATCGTGGAAGCCATCGCCCGCATGCACGGCGGGCG12.857142857142857No Hit
TCCCAAGACTAAATCAGGAAGAAGTTGAATCTCTGAATACACCAATAACA12.857142857142857No Hit
GGTCTCATGCGCATCCGAGTGATGGCATGTTGGCTCTGCTGCAGGAGCGA12.857142857142857No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGAACCACACC12.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGGAATGTTTTTGGCATGTACAAAGAGAATTTTATGCCTGTCTCTTATAC12.857142857142857No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAGT12.857142857142857No Hit
GTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTA12.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTTCGGCATCAAGCGCAAGCGCCGCATCGTCAACATCGTCCCGGCTGACG12.857142857142857No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAT12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)