FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTT38.571428571428571Clontech Universal Primer Mix Long (96% over 26bp)
GAGTGGGATAGGAGAAAGAAAATAGCACATACAAATTCCTAGTAGGGAAG25.714285714285714No Hit
GCCGCCGGAGTGGTCATCGGGCTCTCCTCCCAAGCCTCCCTGGCCGTGGT25.714285714285714No Hit
CCCTTTTCTACTGGGAAAAGCAGTGTTCAGGTGCACAGGTAACGTTAGTT25.714285714285714No Hit
CACACGCACAAGAATACTCCTACAGTTGCCCTGCAACCAAGAAGCTAATA25.714285714285714No Hit
GATCTGAATGAGAATGTGGGATCCATCAGCAAAGATGACAAGTGACTCAG25.714285714285714No Hit
CCCTCGACGTTCCTGGCGAGTCGGAGTCCTGTAGTGCCGATAAGATCGGG25.714285714285714No Hit
CTCGAATGGAATCATCATAGAATGGAATCGAATGCAATTATCGAATGGAC12.857142857142857No Hit
TATCACCAGTCACTGCACTGAAAATTCCAAAACTAGAAGTAGAATAAGGC12.857142857142857No Hit
CAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATACACATCTGACG12.857142857142857Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12.857142857142857Clontech Universal Primer Mix Long (96% over 26bp)
GCATAAAATTCTCTTTGTACATGCCAAAAACATTCCGCTGTCTCTTATAC12.857142857142857No Hit
CGGCCGATCTTGCCCTCCTTGCGGATCTCAATCTTGGAGGCCTGTCTCTT12.857142857142857No Hit
GAGTACATGGGTGAACCACACCGTTCACTCTTCCCTACACGAGCGGCCGA12.857142857142857No Hit
GTGAGGCAATAGAGGCGTCCGAGACTGATCCTGAGATCCCAGAGCCAGAG12.857142857142857No Hit
GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCG12.857142857142857No Hit
CACGCTGGTGGTGCCGTCGGCCAGCGGAATGAACCAGAACCAGCCGTGCG12.857142857142857No Hit
TTGATACCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTG12.857142857142857Clontech Universal Primer Mix Long (96% over 26bp)
GTTAACAAGAATATCCAGGAATTGAACTCAGCTCTGCACCAAGCGGACCT12.857142857142857No Hit
CCTGCTGCCTCAGCCTGCTCGAGGACCTCGGAAGCAACGCCCTGGCCGTC12.857142857142857No Hit
GTATCAGGATGATGCTGGCCTCATAAAATGAGTTAGGGAGGATTCCCTCT12.857142857142857No Hit
GAGTTCGGTCAGCGCTTGGCCACGGTGAAGCCGATGCGCGTCTCGCCGCC12.857142857142857No Hit
GCTCGCCACTCCTTAGTCGAGGCAAGACGTGCGCCCGAGCCCCGCCGAAC12.857142857142857No Hit
ACTCTCCTCCAAATGCCTATCCTTATCTGATACAGATCCTGCATGATGCG12.857142857142857No Hit
AAGGAAGACAAGAAAACAAGACTGAAGAATTCTTCCAGATTGTAAATGCA12.857142857142857No Hit
GTATGTGCTCGGCCCGCACGTGGCCGCGGCCGCCCAGGAGGCATACCTCT12.857142857142857No Hit
AGCGGGAACAGGCAGAGCAAACAGAGAGAAAACGGCAACGCCGACTTCCC12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)