FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATATTACAGCCGCAACTACTGACATGACGGGTGATGGTGTTCACAATT24.878048780487805No Hit
CGGTGAAGTCGGCGACTTCGCCCTGCCCGCCCTTCAACCCTTCGGCGATG24.878048780487805No Hit
ACCCACCTCAACCTCCCAAAGTGCTGGGATTACAAGCATGAGCCACCATG24.878048780487805No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAGAGGAAAATGGCTAGAAAATGTTGAGATAATGATGAGAAAGAGG12.4390243902439024No Hit
CGCCGTCTGAGATGGGCGCGACAGCCATCCGGCGCGTGAACGCACCGAGG12.4390243902439024No Hit
CTGGTCGGTTGAATCACTGCGGTTGCTCCGTTGGTTGATCAGGCGGGCCG12.4390243902439024No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.4390243902439024No Hit
GCACATAGATGGTATTGAAAGCCACAATAATTTGGGAATAAATGTTGAGA12.4390243902439024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATAGGAAAGGTGTCTG12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAGCAGAGCAGCTCCCTCGCTGCGATCTATTGAAAGTCAAACCCTTGTGT12.4390243902439024No Hit
GGTCGTGTAGGGAAGGGTAGATCTCGGTGGTCGCTGTATCTGTCTCTTAT12.4390243902439024Illumina Single End PCR Primer 1 (95% over 21bp)
GCCTTGCCTCCCAAAGTGCTGGGATTACAGGCATTAGCCACCGCACCCGG12.4390243902439024No Hit
GCTTGGCTCAGAGGCAGGAATTAGAGGCAGGGAGGCCCTGGAGCCCANGA12.4390243902439024No Hit
GTTATATAGTAAATTCTCACTCCAAATCAGTTCCTCAGAGATAGTGTTTT12.4390243902439024No Hit
GACTACAGGCACATGTCACAGTGCCCACTGAGATGCTATTGTTTTAAGCC12.4390243902439024No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGCTTCCCATGTACTCTGCGT12.4390243902439024Clontech Universal Primer Mix Long (95% over 23bp)
AGTTTACCCTCTGGATGCTCTGTTGGCACTGCTTCCTTTCCATCTGCTGT12.4390243902439024No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATA12.4390243902439024No Hit
CATTTTTATCTTGCTAGGGCCCTTTCTGGAGTCTCATTAATTAAAGCTCT12.4390243902439024No Hit
GTTACCCAGGCTGGTCTTGATCTCCTGAGCTCAAGCGATCCGCCCAGTTC12.4390243902439024No Hit
CCTGTCGATAGCATTGCTGCTAGATCACCTTGACCGCCCTGAGGATGCAG12.4390243902439024No Hit
ATAACGTGCGATAACGCATAAGAACGAGCAAGAACACATGTTTGGATAAA12.4390243902439024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCAAAAGCTAATGATTATTCAGCTACGCAAATCATCGTCGGGCAGCCA12.4390243902439024No Hit
TGGTGCTGGGGAAGTTCTGCAGAACTACGTAGAGTGTGTCTCAAAGGTGT12.4390243902439024No Hit
TCATCAGACTCCTCGGATTCTTCTTTCTTTGCTTCCACTTTCTTCTCCTC12.4390243902439024No Hit
CCGAGCGCGTGGCACCCACCGCGGCCCCCGCGGTCACCCAGTCCGGCGGC12.4390243902439024No Hit
GTTTTTCTAGAGTGCCACTCACCTTCATGTAACTAAACCTTACCCTATTC12.4390243902439024No Hit
TAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGAC12.4390243902439024No Hit
GTGTTGAATGGAGTCGTATATGTCGTGACCGATTGCCTCATCGTCGACCG12.4390243902439024No Hit
GGTCATCTAGTAGCAGGCACCCAAATCAGGCAACTCCCAAGAAAAGTGGT12.4390243902439024No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT12.4390243902439024Clontech SMART CDS Primer II A (100% over 26bp)
ATTCCACTTGATTCCACTCCATTCCCTTCCACTGCATTCCATTCGATTCC12.4390243902439024No Hit
CAATAATATCGCCACCGACAAGAAGGCGGTGATCCCAAGCCACGGGGCCT12.4390243902439024No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCACTACCCGATCTCGTTTG12.4390243902439024No Hit
GTGAGTTGAATGCAAACATCACGAAGAGGGTTCTGAGAATGCTTCTGTTT12.4390243902439024No Hit
GATGGACAAAGCCCTTAATGGAGAAGTTGGAAAAACTGACAGTCTAGGGA12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)