FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCAGTAAGCTGAGATCGTGCCATTGCACTTCAGCCTGGGGAACAAGAA24.878048780487805No Hit
GCCGAAGCTGGTGGCGTCGTCGCTGGTCGGCGAATCCTCGGCCGGCAACC24.878048780487805No Hit
GACATTACCTTTTTCATCCATCGGAGTAACAATCGCGACAATACTTCCCG24.878048780487805No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAGTAATGGGCGTTTAAACCAAGGAT12.4390243902439024No Hit
GCTACGATAAGAACGTCAATATGACGTGCCTGACGAAGAATGGCCTTGAC12.4390243902439024No Hit
GGCCATTCTTTAAACCACTTTTCTTGGGAGTTGCCTGATTTGGGTGCCTG12.4390243902439024No Hit
GTTAAGTAGAGTTAATGCACCTGGGACCAGCCTTTCAGCTAATGATGGAA12.4390243902439024No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
ATACAGCGACCACCGAGATCTACCCTTCCCTACACGACCCTGTCTCTTAT12.4390243902439024Illumina Single End PCR Primer 1 (95% over 23bp)
ACACCTTTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTG12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GCCGGAACGTCCACCGCTCTGACGGGACCCGCCCTGGCGCTGGCCACCAG12.4390243902439024No Hit
CCATGCGTCTAAGGCCTGCATCGGCGCTTCCGCATGCTTCGCTGATCAGC12.4390243902439024No Hit
CAACGGAATAGAACAGACTCGAATGTAATGGATTGCTATGTAATTGATTC12.4390243902439024No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GAATAAATAAGTTTGGAGATAGAGCCGATCCTGGGTGACAAAATCCAAGT12.4390243902439024No Hit
CCCATGGAAGTGTGGAGACTTTATCTGGGTGTTGAATGATGAGTAAGAGT12.4390243902439024No Hit
CTGTCACACATGCTAATCATGACTAGGTTCTATAGCAGATGCATGTATAA12.4390243902439024No Hit
ATTGAAAGTCAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGC12.4390243902439024No Hit
TTGCTAAACTAAGAGCTTTAATTAATGAGACTCCAGAAAGGGCCCTAGCA12.4390243902439024No Hit
GAACATATCAACACCAATGCCTGATTGCATCATGCTCTGATGTTCCATCC12.4390243902439024No Hit
CGTCTGGCCTCTTCAATTCCAGCCTGGACGGCAACACGCGCCGCGCCATC12.4390243902439024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG12.4390243902439024No Hit
CCTTCCAGGAGGCCCTCTTTTCCTCCCTAGACTGTCAGTTTTTCCAACTT12.4390243902439024No Hit
TTGGGACTCTATGCTGGGCATCCTGGGCTCCAGGGCCTCCCTGCCTCTAA12.4390243902439024No Hit
GCTGACAAGCGAGAAGCTGAAGCTAAACAGGACAAGGTTGCCCCCGAGAA12.4390243902439024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.4390243902439024No Hit
GTAGAGATAGGGTCTTGCTATGTTGCTTGGGCTGGTCTCGAACTCCTAGG12.4390243902439024No Hit
GAACTAAAACAGAAGCATTCTCAGAACCCTCTTCGTGATGTTTGCATTCA12.4390243902439024No Hit
GGAATGGGTAACACGGGGCCATTCTGCCAGTCGGTATCTTCGAACTTTTT12.4390243902439024No Hit
GCCTTGAGATTGGGGTGGCGAGGATGGCATGACATAGCTTAGATCAGTCA12.4390243902439024No Hit
GCCCTCATTCTGCACGACGATGAGGTGACAGTCACGGAGGATAAGATCAA12.4390243902439024No Hit
CCCCAGGGCTCTGTAGTTGGACCTCTTTTCTCTCAACATTTATTCCCAAA12.4390243902439024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTACACAAT12.4390243902439024No Hit
GAAGTGTCTCTTACAATGATTACAGGATTAAAAAAAAAAGCAAGTTGGGA12.4390243902439024No Hit
GGCGCTTGGCGTCAGCGATCGCAGTATCATCCTGCGTCATATGTTGCCTA12.4390243902439024No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12.4390243902439024Clontech Universal Primer Mix Long (96% over 25bp)
TCGCTGGATGTTTGTACTTCATTCCCTCCTCAGCCCAGCTGGTCCCACTC12.4390243902439024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)