FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA70.30823425803610743Illumina PCR Primer Index 1 (96% over 28bp)
ACGCAGAGTACATGGGACTCTGGGCAGGATCCAACGTCGCTCCAGCTGCT60.26420079260237783No Hit
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACTGTCTC50.22016732716864817Illumina Single End PCR Primer 1 (100% over 22bp)
GTATCAACGCAGAGTACATGGGCAAGCAGAAGACGGCATACGAGATCTTG50.22016732716864817Illumina PCR Primer Index 1 (96% over 28bp)
TATCAACGCAGAGTACATGGGGTCTGGCTGCTCTGAAAAGCCATCTTTGC40.17613386173491855No Hit
CAGCAGAAGCAATATAATAAGCACCAGAAGCACCCATATCGCCAATCACT40.17613386173491855No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTCTGTCTCTTA40.17613386173491855Illumina Single End PCR Primer 1 (100% over 24bp)
GCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTG40.17613386173491855No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40.17613386173491855No Hit
GTACATGGGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGCACTTTGG40.17613386173491855No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT40.17613386173491855No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG40.17613386173491855No Hit
CGACAAGACCGTGTACTTCGCCGAGAAGGTGAGCTCCCCGGTGAAAGTGG40.17613386173491855No Hit
GTCTTCCTACAGCAACTTCCCACTCCAGGTGGCCAGACGGGAGTTCCAGA40.17613386173491855No Hit
CTCTACTTCTTCCTCTGTCATCTTCTCACCCAGTGTGACAAGAACATGCC40.17613386173491855No Hit
TAGCAGCACTGGTCCCCGCGTTGACAGGTGCCCTCCTTAAACCTCTTGCA40.17613386173491855No Hit
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTCTGTCTCTTATA40.17613386173491855Illumina Single End PCR Primer 1 (100% over 22bp)
GTATCAACGCAGAGTACATGGGAAGGTGAACGTGGATGAAGTGCTGTCTC40.17613386173491855No Hit
GCCGGAGTACTTGTGGAAGATGGCCACGAGGAGGCCAATGGCCTGATCCA40.17613386173491855No Hit
TATCAACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACGCCTGTAATC40.17613386173491855No Hit
GGCTAGTCTCGAACTCCTGACCTGAAGTGATCTGCCCACCTCGTCCTCCC40.17613386173491855No Hit
GTCCTCTGTCATGTTGCCATACTTGGTGGCATTCTGCATGGGCCCCTGGG40.17613386173491855No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACCTGTCTCTT30.13210039630118892No Hit
ACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTC30.13210039630118892No Hit
AGGGCAAGGGAGTAGGGTCAGGTCCTCCCCAGCCCAGCTGTACCCTCCTT30.13210039630118892No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA30.13210039630118892No Hit
ATTTCACTGTCGTCCCACCCCACTTCTTCCACCCCACTTCTTCCTTCACC30.13210039630118892No Hit
ACCATAGTCTGTCCTCTTACTCCCTGTACTCGTTGTCTTTCCCTACATTG30.13210039630118892No Hit
ATTTTGGATGGAGCTGGAGGATATTATTCTAAGTGAAGCAACTCAGGAAT30.13210039630118892No Hit
GATCTACACTTTTTTTTTCGTCGGCAGCGTCAGATGCTGTCTCTTATACA30.13210039630118892No Hit
ACCTCGGAGAGGAGCCACCCGAATCCGGCCGTGGGGGTGGGGGCTGAGGG30.13210039630118892No Hit
GTGCTTGTTCGGGGCGCTCCGCTGGCTTCTTGGACAATTGCGCCATGTGT30.13210039630118892No Hit
CGTCTCTACTAAAAATACAAAATTAGCCAGGCGTGGTGGCACATGCCTGT30.13210039630118892No Hit
GGCTCGGAACGAGACTGCACGGATTGTTTTAAGAAAATGGCAGACAAACC30.13210039630118892No Hit
GTGATCTGCCCACCTCGTCCTCCCAAAGCGTTGGGATTACAGGCGTGAGC30.13210039630118892No Hit
TCAACGCAGAGTACATGGGTGCAGGGGAGGCCTGCCCACTGTTTTGTCGA30.13210039630118892No Hit
GTATCAACGCAGAGTACATGGGAGAGCGGGTGTCAGACTGAGGATTTCGC30.13210039630118892No Hit
CTTCCACAAGTACTCCGGCAGGGAGGGTGACAAGCACACCCTGAGCAAGA30.13210039630118892No Hit
TGATAATTCCATTCGATTCTATGCGATGATTCCATTCCATTCCATTTGAA30.13210039630118892No Hit
GTTAGAGCGTCTCCCGGAAGGATGGGCCGGTCTCGGAGCCGGAGCTCGTC30.13210039630118892No Hit
GTAAATTCATTGCTGCTGTCTAAAGAGACTAAGAGTGCTAAGGAAATTCC30.13210039630118892No Hit
CCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACCGCTCCAAGCC30.13210039630118892No Hit
GTGCCATTTCCTTCCTTGTCAAACACCCGAAGTCCTTCGACATAATCCTC30.13210039630118892No Hit
GTCTTGGAGAGTGATGCAAGAAGTGAATCCCCCCAACCAGCACCAGACTG30.13210039630118892No Hit
CCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTGAGGGCTT30.13210039630118892No Hit
GTTTCGCTCTTATTGCCCAGGCTGGAGTGCAATGGTGCAATCTCGGCTCA30.13210039630118892No Hit
GTCATCCTCGTCGGTCTTCTGCTTCTTGGTATCGACATCGTCATCCTCAT30.13210039630118892No Hit
GCGCGGCCTTCGTTGTCAGTAGTTCTTTGATGTGAAAGGGGCAGCACAGT30.13210039630118892No Hit
CGCCAGAGTCCCTGAACTCTCGCTTTCTTTTTAATCCCCTGCATCGGATC30.13210039630118892No Hit
GTCCGGACATCAGGCACAGCAGCTGCACTTCTCTGATGCCCCTTTGCAGA30.13210039630118892No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGG30.13210039630118892No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA30.13210039630118892No Hit
GTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCG30.13210039630118892No Hit
GTTGTAGTACCGGTAGTAACCTCTTTGAAACGCTCCGAAAATGCCACTAG30.13210039630118892No Hit
GAAAACATTGTTAGTGAACAACAACAGAATATGCCGTATAGGTGAGGGAC30.13210039630118892No Hit
CCGCGGCTGCACTTGCTCTCCGGGGCCCCAGGGCGCCGGCCCAGCATGGG30.13210039630118892No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCATGGCGACTACCA30.13210039630118892No Hit
GGCCAGCCTGCCACGGCCGCTGCCACGGATACAGAGCCTGGGCTGCAGCG30.13210039630118892No Hit
CTCCCAAGTAGCTGGGATTACAGGCACCCGCCACCACACCCGGCTAATTT30.13210039630118892No Hit
ACCCGATGACCAGAAAAATGTTCCTGCCAAGCGGCTACAGTGTTCTTCTT30.13210039630118892No Hit
GTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT30.13210039630118892No Hit
AACCAGCGTGAGCTCGCCCGCCAGAAGAATATGAAAAAGCAGAGCGACTC30.13210039630118892No Hit
GTATCAACGCAGAGTACATGGGAATGATACCTGTCTCTTATACACATCTC30.13210039630118892No Hit
CTCTTTGCATTTGCAGGAGCAGGCGCAGGTGCAGGAGTCACCGGCGGCGC30.13210039630118892No Hit
CACCCGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAACCCT30.13210039630118892No Hit
TTGGTGCTCACGTGTCCTTAACACCTGATCTGGCCCAAGCTGGGCTCCCT30.13210039630118892No Hit
CAAGGAATGCATGAGAGCCCTAGGATTTAAAATATGAAATGGTGGTCTGC30.13210039630118892No Hit
TCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGCACCCGCCACCA30.13210039630118892No Hit
CGTCAGACGCTGGCCTCCAAACACACAGTCATCATAGGGCAGCTCGTCCT30.13210039630118892No Hit
GTTCTGGAAGCACTGTTCAGCGATTTCAACATGCAGGAATACCCCGATTT30.13210039630118892No Hit
TGGAAATGTAGCATTTCAAGCTTTAGTCAGGGTAGATTATTCCTTCTTTA30.13210039630118892No Hit
GCCTTGCAATTTCAGCATCCTGCAGCTTCGAGCCAATGGTGAGCTCCTTC30.13210039630118892No Hit
CTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGC30.13210039630118892No Hit
CCCGCGCCGGGCTAAGAAGGCAGTCTTTCTTCTTCACCTTAGATCCCTCT30.13210039630118892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13210039630118892No Hit
GTGTTTGAGGTGGGCAGATGGGCATGGCATCAACCAAAGAAATTGTTGTG30.13210039630118892No Hit
GTGACATACTCCTGGAAGTTCACCTCCTGGTCCTTGTTCCGCTCCAAGCC30.13210039630118892No Hit
GCAAGGGGGAAGAAAAGGGTCCCCTAGTTGAGGATAGTCAGGAGCGTCAA30.13210039630118892No Hit
CCCGACCCCCTGCAGATGCCAACCTTCCCGCCAGACTACGGCTTCCCCGA30.13210039630118892No Hit
GGATGTGGCCTGGCCTCAGTTCCATCTTCCTTGATGCTACCCTCCTGGCT30.13210039630118892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)