FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG80.3522677234698371Illumina PCR Primer Index 1 (96% over 28bp)
ATCCCAGAGAAGGTGTCAGAGGCCTGGTTAGCAGTCTTGTCGATGGTTTC70.30823425803610743No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA70.30823425803610743No Hit
TCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTGCC70.30823425803610743Illumina PCR Primer Index 1 (96% over 28bp)
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCTC50.22016732716864817Illumina Single End PCR Primer 1 (95% over 22bp)
GTGCTAGATGGCGCCTGGAAGGGGGGTCACCCAAGTCTCCCTGCTGTCAT50.22016732716864817No Hit
TGGCTGAGCAGAGGGAACTAGGGGTCGGCAGAAAGGATTATGGGTGGAAA50.22016732716864817No Hit
GTTCAGCACCTTGAGGAAGTCAGACGTTTTCGCCCGCATACGTGTGTGAA40.17613386173491855No Hit
CTGATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTT40.17613386173491855No Hit
ACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCTCTTATA40.17613386173491855Illumina Single End PCR Primer 1 (95% over 22bp)
ACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGTCTCTTA40.17613386173491855Illumina Single End PCR Primer 1 (95% over 24bp)
CACTTCATCCACGTTCACCTTCCCATGTACTCTGCGTTGATACCTGTCTC40.17613386173491855No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17613386173491855No Hit
CCCCTCGACCGCTCGCGTCGCATTTGGCCGCCTCCCTACCGCTCCAAGCC40.17613386173491855No Hit
CGTCTGAACTCCAGTCACAAAAAAATCTCGTATGCCGTCTTCTGCTTGCC40.17613386173491855RNA PCR Primer, Index 19 (96% over 28bp)
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA40.17613386173491855No Hit
GGCCACCACCGCCTACTTCCTGTACCAGCAGCAGGGCCGGCTGGACAAAC40.17613386173491855No Hit
CCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAA40.17613386173491855No Hit
CCCTGGGGAAGGACTGGCACAAGTTCTGCCTCAAGTGCGAGCGCTGCAGC40.17613386173491855No Hit
GAGTACATGGGGCTGGGCGCGGTGGCTCACGCCTGTAATCCCAACGCTTT40.17613386173491855No Hit
GCATAGGAGAAGAGCTGCTGCTTGAGAGACGGGTTCCTGGCATAGCCAAT40.17613386173491855No Hit
TTTCGGAGCTGGTGTCTACGGCTGCGTCTGACATGGTGGGGCACGCCGGT40.17613386173491855No Hit
GGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAA30.13210039630118892No Hit
TTTGAATTCTGTCTTCAGTGCATCTCTCACTGCTTTTGCACAGATCTGGG30.13210039630118892No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA30.13210039630118892No Hit
CAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACT30.13210039630118892No Hit
CCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTT30.13210039630118892No Hit
GTGCTCATCTGGCTGTGGTTGATATTGTTGGAACCATTGATGCTTCAAGC30.13210039630118892No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA30.13210039630118892No Hit
GTATCAACGCAGAGTACATGGGTGGGCGCGGTGGCTCACGCCTGTAATCC30.13210039630118892No Hit
CCCATGACGGTGCCATTTCCTTCCTTGTCAAACACCCGAAGTCCTTCGAC30.13210039630118892No Hit
CCCTCTCTTCTTCTTCACTTTCTTGGAGAGGCAATTTGTCATGGATTACA30.13210039630118892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.13210039630118892No Hit
AATATGCACTGTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTT30.13210039630118892No Hit
GTTTAATACGCTTTGTCTGGTAGTGCTTGGGTGCCTGTGGTTGGTTTCTC30.13210039630118892No Hit
TTTTTATACCTTGCCCCCCTCTTACCCTCCCCACTTTGAGTCTCTAGTGT30.13210039630118892No Hit
GTATCATTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTG30.13210039630118892No Hit
AGTTAGTTCTGATTTCAGGTCATCCAGAAGATCCCTGCCAAACAGAGTCT30.13210039630118892No Hit
GGGCAGATGGGGTCCCACCTACTCCTCCCCTCGCACAGGGGCAAGGCGGC30.13210039630118892No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA30.13210039630118892No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA30.13210039630118892No Hit
GTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTCGAGCCAATGG30.13210039630118892No Hit
GCGGACACCAGGACTCCAAAATGGCGTCAGTTGGTGAGTGTCCGGCCCCA30.13210039630118892No Hit
CCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACGA30.13210039630118892No Hit
GTATCAACGCAGAGTACATGGGGAGGAGGACAGGAGCCTTCAAAGCAGCA30.13210039630118892No Hit
GGAGTGCAATGGTGCAATCTCGACTCACCGCAACCTCCACCTCCCGGGTT30.13210039630118892No Hit
CCCCACTTCTTCTTTCACCAACATGCAAGTTCTTTCCTTCCCTGCCTTCC30.13210039630118892No Hit
GCCCTTGAGGGCTTCATTGTAGATCAAAGCCAAGGCCCCCAGGAAGGTGA30.13210039630118892No Hit
GTGGGACTGGCTCCAGGCACACAGGCGAAGGGCAAGAGGGTTGGACACGA30.13210039630118892No Hit
GGCAAAGGTTGTTCGACTAGCAAAGAAGGCTCAGCAGCAAGTAGTAGCAG30.13210039630118892No Hit
ATTTTGTCCACAAAGTCTTTGCACTCTTCCTTCAGCTCTATGGTCTGCTG30.13210039630118892No Hit
GAGGACCCCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTG30.13210039630118892No Hit
CTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGA30.13210039630118892No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCTGTCTCTTAT30.13210039630118892No Hit
TCTCCAACCTCACCGCGGCTCGAAATGGACCCCAACTGCTCCTGCGCCAC30.13210039630118892No Hit
AACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGTGTGGTGGCGGGT30.13210039630118892No Hit
GATTACAGGCATGTGCCACCACGCCTGGCTAATTTTGTATTTTTAGTAGA30.13210039630118892No Hit
GAGAATCAGGAGACAGGCTGACTGGAAAACCCCGGCGGCCGATGGCGGAG30.13210039630118892No Hit
CATCTGACGCTGCCGACGAAAAAAAAAGTGTAGATCCTGTCTCTTATACA30.13210039630118892No Hit
CAATGGAAATGAAAAACCAGCCCCACTTGCCATGAACAGCCCCAACTGTT30.13210039630118892No Hit
TGGTAGCAGGGCGCCGGCCGCCGAGCCGTCTCAAGTTTAAACTTACACGA30.13210039630118892No Hit
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA30.13210039630118892No Hit
CCATTGGCTCGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGAC30.13210039630118892No Hit
TCTTGCAGGTGGCTCTTCCTCCACATCACGACTGGGGTCTCGAAGAGGAC30.13210039630118892No Hit
TCTTTGCATCTTACAAAGTTAAACAGCTAAAAGAAGTAAAATAAGAAGGC30.13210039630118892No Hit
GGTAATTTCCGCTCCATTTTCCAGCAACAACCTGCCGGTCCACAGCAGAA30.13210039630118892No Hit
GAAACGAATGGAATCATCATCGAATGGAAATGAAAGGAGTCATCATCTAA30.13210039630118892No Hit
GGAACACACAGCTACAGCACTCTATGTACAAGCACATTGACGCTCCTGAC30.13210039630118892No Hit
GGCAGAGTCGACAAAACAGTGGGCAGGCCTCCCCTGCACCCATGTACTCT30.13210039630118892No Hit
CCATGTTCCAGGCGGCCGAGCGCCCCCAGGAGTGGACCTACAAAATCCTG30.13210039630118892No Hit
CCCGTTAGCAGGGGCGTCTCTTCCATTTTCTGCCTCTTCCACAACTTCCT30.13210039630118892No Hit
CTGCAGGGGGTGGACAAACTGGAGTCTGGAGTGGGCAGAGCCCCCAGGAA30.13210039630118892No Hit
GTGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTT30.13210039630118892No Hit
TCCTTGCTCGCCGCAGCCGCCTCCGCCGCGCGCCTCCTCCGCCGCCGCGG30.13210039630118892No Hit
CCGAAGACCAGACCGCAGAGTTCAAGGAGGCCTTCCAGCTGTTTGACCGA30.13210039630118892No Hit
CTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTC30.13210039630118892No Hit
CCTTCAGCTCCTTCTTGCTCAGGGTGTGCTTGTCACCCTCCCTGCCGGAG30.13210039630118892No Hit
GGTTTCCAAGCTTTGCACAACTCGTGCACAATTCACTTTTTAAAACAATG30.13210039630118892No Hit
GTCCAGATCACCCAGTTCCACGAGACTATTATTGGTGAGAATGAGTTCTG30.13210039630118892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13210039630118892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)