FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCGCAACCATGTCTGACAAACCCGATATGGCTGAGATCGAGAAATTC70.303161541792984No Hit
CACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTTAATAGCCT60.25985275010827197No Hit
ATCTTGTTCACTCTCTGGATTATACAGTTCATCATCTTGTTCTGGTACTT50.21654395842356No Hit
TCGAGCAGGGCTGTCCGGACATCAGGCACAGCAGCTGCACTTCTCTGATG50.21654395842356No Hit
GTCTGACACCCAGGCTGGAGTGCAGTGGTACGATCTCGGCTCACTGCAAC50.21654395842356No Hit
GTTTTCCCTTGAAGTCATGAACGGTTGGTTCTGCTGTGACTTTGGGGCTT50.21654395842356No Hit
ATCAACGCAGAGTACATGGGGAAGCGGAGAGGCGGTCGGGATCCGCTGCG50.21654395842356No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA40.17323516673884798No Hit
GCCGTAGACACCAGCTCCGAAATCACCACCAAGGACTTAAAGGAGAAGAA40.17323516673884798No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17323516673884798No Hit
GAGTTGTGGCTGTGCCCATGGTGCTCAGTGCCATGGGCTTCACTGCGGCG40.17323516673884798No Hit
GTGGAGGGGACCGCCCAGGAAGCCGTGTCAGCGGCCGGAGCGGCAGCTCA40.17323516673884798No Hit
GGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCTGAGATTGC40.17323516673884798No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTGT40.17323516673884798Illumina Single End PCR Primer 1 (95% over 24bp)
GAGCTTTTGTCTTCCAATTTCACTTTTTTTTCTGTTTTCGATATTCCTGC40.17323516673884798No Hit
GTACATGGGACACACCATATGTGTCCTGTTCCAGTGCGCGGGTCTGTGGA40.17323516673884798No Hit
GTACATGGGCAAGCAGAAGACGGCATACGAGATCTTGCTTCGTGACTGGA40.17323516673884798Illumina PCR Primer Index 1 (96% over 28bp)
CGACATCGTCATCCTCATCATCTTCAGCTGCCCGCTTGCCCGTAGCTGAC40.17323516673884798No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCCTG40.17323516673884798Illumina Single End PCR Primer 1 (100% over 24bp)
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA40.17323516673884798No Hit
GAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTA40.17323516673884798No Hit
CCCGCGCCGGGCTAAGAAGGCAGTCTTTCTTCTTCACCTTAGATCCCTCT40.17323516673884798No Hit
ACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGGGTGTG30.12992637505413598No Hit
TGTTCACTCTCTGGATTATACAGTTCATCATCTTGTTCTGGTACTTCAGT30.12992637505413598No Hit
CCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGA30.12992637505413598No Hit
GTCGATGAGCACGATGCAATTCTTCACCAGGGTCTTGGTACGAACCAGCT30.12992637505413598No Hit
CACCAGCAAGTAGGCGGGCAGTGGCCACAGGACTTCCTGGCAGGACAAGG30.12992637505413598No Hit
GTCTGGGGACGAGATGATTTTTGATCCTACTATGAGCAAGAAGAAAAAGA30.12992637505413598No Hit
CTTCAAGCAATCAAGCAGTAAATAGTGAAGATACGAACAAAACGCTTAAG30.12992637505413598No Hit
GTACATGGGACACCGGTACTTAAGCGCGGACCGGCGTGTCCTTGGACTTA30.12992637505413598No Hit
GCCTATGGAAACAGCGCCGCGCACAGAAAAGGAAACTTCATTCCGTCTGT30.12992637505413598No Hit
CTCCCGGGTTCAAGCAATTCTTCTGCTTCAGCCTTCCCAGTAGCTGGGAC30.12992637505413598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA30.12992637505413598No Hit
CATCCAAACTCATTAGACTACAAGGATCACGGAAACTGTTGGTTGACAAT30.12992637505413598No Hit
TGCCGAGGCTGGCATAGATTTGGCTGTCTCCGCTCATAGCTGCTTTTGGC30.12992637505413598No Hit
TTGGTATTCAGTGTGATGACCCCATGTACTCTGCGTTGATCTGTCTCTTA30.12992637505413598No Hit
CCCCATGAAAGATGGAAATTAGAATCTCGGGCTCTTATTTTCTTCATGGT30.12992637505413598No Hit
CACTTGGCACAGCTCATGGGGCAGCAGGAGCAGCAGCTCTTCTTGCAGGA30.12992637505413598No Hit
GCTTAGACGCGACTCGCGCGGGCGCCGCAGCCGCGGCAGAAGCACGGGCC30.12992637505413598No Hit
CCAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAG30.12992637505413598No Hit
CTGCAACCTCCACCTCCTGGGTTCAAGCAATTCTCCCACCTCAGCCTCCT30.12992637505413598No Hit
CTTTTTCTGGAGCCCTCTTTCAAGTCTGGCTTATATCGTAGTTCTGTATC30.12992637505413598No Hit
GCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTT30.12992637505413598No Hit
CTCCTGAAGGAACATCCTTCTGTAAGCCTTGCTTTTCCTCCTGTAGGCTG30.12992637505413598No Hit
TTTTAATCCCCTGCATCGGATCACCGGCGTGCCCCACCATGTCAGACGCA30.12992637505413598No Hit
ACGCAGAGTACATGGGGCCGGGCGCAGTGGCTCACGCCTGTAATCCCAGC30.12992637505413598No Hit
GTACACGATGTGGCATAATATGCATTTTCGTTCTCGTACCATAGTGACCA30.12992637505413598No Hit
ATGTTGATGTTGGATAAGAGAATTCTCTGCTCCCCACCTCTAAGTTGCCA30.12992637505413598No Hit
GATCAATGTCATCCTTGGTGCTGTCTTCGCTCTTGTTTTTCTGTTTCTTC30.12992637505413598No Hit
GTACAGGATGTTGTCAACGATGCCGCCCTTCAGGTACAACGGGATGTCAT30.12992637505413598No Hit
CTTCCACCCCACTTCTTCCTTCACCAACATGCAAGAGAAAAATCCACTGC30.12992637505413598No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC30.12992637505413598No Hit
TCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACTGTCTC30.12992637505413598Illumina Single End PCR Primer 1 (100% over 22bp)
GTATCAACGCAGAGTACATGGGCATACCGGACTCATCCTGTTTAGAGATC30.12992637505413598No Hit
CACATTGACTGTCTGTAGATTTTTGAAAGTGGTAACAGGTACATAGGTAA30.12992637505413598No Hit
CCTGAATGGTAATGCCTAGGTTTTCTTCTAGGGTTTTTATGGTTTTAGGT30.12992637505413598No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA30.12992637505413598No Hit
GCGGTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTAT30.12992637505413598No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA30.12992637505413598No Hit
GTGGCACGATCTCGGCTCACTGCAACCTCTGCCTCCCGGGCTCAAGAGAT30.12992637505413598No Hit
GTGTTCACTCTCACAAAACCCGCTACAGACAAGCTTTCTGGGCACACCTC30.12992637505413598No Hit
GGATCACGGTGGACGTCGGGGGAGCAGGGTTGTGGGGCGCCCCCAGCACA30.12992637505413598No Hit
TGGCCACATAGTCTTTCACCTCATCCAAGTCTCCGTTTTTCAGGGCCCAC30.12992637505413598No Hit
CCTACAGAGATGGTCCAGGGTTCCGCTTGGCGATGCTGCACACATTCAGC30.12992637505413598No Hit
TCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAGGTCAGGAGT30.12992637505413598No Hit
GATGTATGACATCTGTAAATCCTTCTTGTACAGCGCTGCTAAATTTGACA30.12992637505413598No Hit
AAAAAGTGTAGATCTCGGTGGTCGCCGTACCCATGTACTCTGCGTCTGTC30.12992637505413598Illumina Single End PCR Primer 1 (96% over 29bp)
CTCTTTCTGCAAAGGCACCTGAATGTGTCTGCGTCCCTGTTAGCATAATG30.12992637505413598No Hit
GGTGTCAGACTGAGGATTTCGCTTCCCCTCCAAGACGCTGGGGGTCTTGG30.12992637505413598No Hit
CCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGAG30.12992637505413598No Hit
CGGTCAGGGTTGTACATAAAAAAAATCCAGGTTTGTGGAAGTCGCGTTCT30.12992637505413598No Hit
ACCGAGATCTACACTTTTTTTTCGTCGGCAGCGTCAGATGTGTAAAAAAA30.12992637505413598No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT30.12992637505413598No Hit
CGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCTGAG30.12992637505413598No Hit
ATCTCGGCTCACCGCAACCTCCACCTCCCGGGTTCATGCGATTCTCCTGC30.12992637505413598No Hit
TATCAACGCAGAGTACATGGGATTGTGCCTTAGGAAAAGCATTTGCTGTG30.12992637505413598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12992637505413598No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC30.12992637505413598No Hit
GTTGGACACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTG30.12992637505413598No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC30.12992637505413598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)