FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA120.5197055002165439No Hit
CCTCATCATCTTCAGCTGCCCGCTTGCCCGTAGCTGACTCAGCTTCCTCA50.21654395842356No Hit
GGCGGCGGCAGCGGGAGACGAAAAAATGAAGAGGGCCGCGGGAAAGTAGG50.21654395842356No Hit
GTTGTATGGAAAAAAAATTCCAAGTTTGTGCAGGTCACTCTTTCTATATC50.21654395842356No Hit
TTCCCAAGTTTTGTGATACTTTTCTCAAAGTTCCTAGATGTGACTTTAGG50.21654395842356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA40.17323516673884798No Hit
GGCTGGAGTGCAATGGTGCAATCTCGGCTCACCGCAACCTCCACCTCCCG40.17323516673884798No Hit
CTCCTGTTCTTTGGCAGCACCAGCTCCTTCTATTTCTGCAGCCAAGGCAT40.17323516673884798No Hit
GACTTAAGTCGTGGTTCAGAAGTTACAGCACCGGTAGCCTCAGATTCCTC40.17323516673884798No Hit
GGCTGGAGTGTAGTAGCGCAATCTCAGCTCACTGCAACCTCCGCCTCCCG40.17323516673884798No Hit
CCTGATGTCAGGAGTTCGAGACCAGCCTGGCCAACATAGCAAAACTCTGT40.17323516673884798No Hit
CCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTC40.17323516673884798No Hit
CTGCAGGGCGAGGGGCAGGGAGCTAGTAGAACCTCGCAATGACAGCCGCA40.17323516673884798No Hit
GTGAAAGGAAGGGAGAAAGGGAAAAAGCGGGAAGAGTCGAGAAGGCTGAG40.17323516673884798No Hit
GAGAATCAGGAGACAGGCTGACTGGAAAACCCCGGCGGCCGATGGCGGAG40.17323516673884798No Hit
GGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGATCCTG40.17323516673884798Illumina Single End PCR Primer 1 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA40.17323516673884798No Hit
CTTAAAGGAGAAGAAGGAAGTTGTGGAAGAGGCAGAAAATGGAAGAGACG40.17323516673884798No Hit
GATCTCGGTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGT40.17323516673884798Illumina Single End PCR Primer 1 (100% over 24bp)
CTTATTGCCCAGGCTGGAGTGCAATGGTGCAATCTCGGCTCACCGCAACC40.17323516673884798No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC30.12992637505413598No Hit
GCATCTTGGAGAAGACCACGAGGCTGGACTCAGCGAGCTCACCTTGTCCT30.12992637505413598No Hit
GGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCT30.12992637505413598No Hit
GCTCCTGCGCCACTGGTGGCTCCTGCACCTGCACTGGCTCCTGCAAATGC30.12992637505413598No Hit
CGTCTGAACTCCAGTCACGAAGCAAGATCTCGTATGCCGTCTTCTGCTTG30.12992637505413598Illumina PCR Primer Index 1 (96% over 28bp)
CGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAA30.12992637505413598No Hit
GTGTTAAGAGGCCAAGTGCGACGCGCGTATCCGGGCAGACGGACTGACGG30.12992637505413598No Hit
CCCCCAGAGGGTGTCTCCATCTTCCCTATTTATTTTCAGCCCTTGAGGGC30.12992637505413598No Hit
GTTTAAAGAAGTTCAAGTCATCTAGATCATCAGAAGCATCTTTTTTCCTA30.12992637505413598No Hit
GAGTACATGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC30.12992637505413598No Hit
TCTCCCTGCTGTCATTTCAGGAGGCCGACCCAAGTCTCCCTGCTGTCATT30.12992637505413598No Hit
GTCCCAGCTACTGGGAAGGCTGAAGCAGAAGAATTGCTTGAACCCGGGAG30.12992637505413598No Hit
TCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCG30.12992637505413598No Hit
GTACATGGGAGAGCAAGAGAAAGACCTAGAGCAAAATGAAATAAAAAATA30.12992637505413598No Hit
CTCTTTTTAGTCGCTGGCACAACGAACACTCCCGCTCCGAAGCCGGACGT30.12992637505413598No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.12992637505413598No Hit
CCCTTTCCCCAGCTGTCCACTGAAACGTGAAGTCCTGTTTTGAATGCCAA30.12992637505413598No Hit
CCAAGAAGGCGGGGCAGGGTTTGAGTCAACCAGACCCGGCATCTTTCGCG30.12992637505413598No Hit
TATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGACTGTCTC30.12992637505413598Illumina Single End PCR Primer 1 (95% over 22bp)
ATCAACGCAGAGTACATGGGGTCATCACACTGAATACCAACTGTCTCTTA30.12992637505413598No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACTACTGCTAAGACAGCTAA30.12992637505413598No Hit
ACCGGGAGGTCCAGGCCAAGTCACTGGTCCCTGGGCTCGGGCCCTGCCGA30.12992637505413598No Hit
CCATACCAGATCCACACAGCAACTCCAGTCCTTGCATCATGAGATCGGAA30.12992637505413598No Hit
TATGTGTCCTGTTCCAGTGCGCGGGTCTGTGGAGAGCCGGGTGCGAGCGG30.12992637505413598No Hit
GTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGACT30.12992637505413598Illumina Single End PCR Primer 1 (96% over 25bp)
CTCCGGCTTCGCGCGGCCCTGCCTCCCTCTCGCCCGGCGGCATCCGCTTG30.12992637505413598No Hit
GTATCAACGCAGAGTACATGGGGAATCCCAACATTTTGGGAGGCCAAGGC30.12992637505413598No Hit
GCATCGGATCACCGGCGTGCCCCACCATGTCAGACGCAGCCGTAGACACC30.12992637505413598No Hit
ATCCTGCTGCTGGGCATCTCGGTGTGGATTCGCCTGACGCTGTCGGAGTC30.12992637505413598No Hit
GAAGACTGGGCCTAGGCCGCTGGATGCTGGAGTGAAAGGAAGGGAGAAAG30.12992637505413598No Hit
GCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGATGC30.12992637505413598No Hit
GTTTTAGGTCTAACGCTTAAGTCTTTAATCCATCTTGAATTGATTTTTGT30.12992637505413598No Hit
TCACTGAAGGCTATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGG30.12992637505413598No Hit
GATTCTTATTTTCAGTAGGGAAGTAAGAAGTTGCAGCTCAGTGCACATAA30.12992637505413598No Hit
GAACAAGGACCAGGAGGTGAACTTCCAGGAGTATGTCACCTTCCTGGGGG30.12992637505413598No Hit
CGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCT30.12992637505413598No Hit
GTAAATTCCGGGAAATTCAACAGGAGCTTTCCAGTGGAAGGAATGAAGGC30.12992637505413598No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC30.12992637505413598No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG30.12992637505413598No Hit
GAGTGCAATGGTGCAATCTCGGCTCACCGCAACCTCCACCTCCCGGGTTC30.12992637505413598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT30.12992637505413598No Hit
GCGTGAGCTCGCCCGCCAGAAGAATATGAAAAAGCAGAGCGACTCGGTTA30.12992637505413598No Hit
GTACATGGGGAGGTGACTCCGGCGGAAGAGGACAAGGCAGAATGCAGGCC30.12992637505413598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT30.12992637505413598No Hit
TTCTTGTACAGCGCTGCTAAATTTGACACTCAGGGAAAAGCATTCGTCAA30.12992637505413598No Hit
GCAACGACACCAGCAGCAGCGCCGCGAACATCATCGGGATCTCGGTGCGG30.12992637505413598No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT30.12992637505413598No Hit
CTCCCGTGGATGACGGATGAGGACTCTGGGCTGCTGGAATAGGACACTCA30.12992637505413598No Hit
CCAGATGTGCGCATTGACACCAGGCTCAACAAAGCTGTCTGGGCCAAAGG30.12992637505413598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAA30.12992637505413598No Hit
GCGCCCAGCCGCGCCTGGCGCCCGCCGCTGAAGCGCAGGACGCGCCGCCG30.12992637505413598No Hit
CGCTTTCTTTTTAATCCCCTGCATCGGATCACCGGCGTGCCCCACCATGT30.12992637505413598No Hit
GTGCGGGGAGGTAACAAGAAATACCGTGCCCTGAGGTTGGACGTGGGGAA30.12992637505413598No Hit
GCCTTACTTCCCAGAGGCTCCTGCCTGCGCCCGCAGCTGACCTTCTCCTG30.12992637505413598No Hit
GGCTTTGGGGACTAGCGATCCTGGGCTCCACCTGGGTGGCCCTGACCACG30.12992637505413598No Hit
GATCAGTGCTTCTGCAACCCATGAGGTGGAGCAGAATGACTCTGTGAGCC30.12992637505413598No Hit
GGCATAAAGTCCGTTGGCCGACACCTTTCTTTCCTCCGGCCTCGGTAGAA30.12992637505413598No Hit
GCTAAGAAGAAGGGAACATGGTTGGAGGCCCAGTGAGAGAAACAGTGCTT30.12992637505413598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG30.12992637505413598No Hit
GGAGCAGGCTCCCGGACCCAAAACTGAAGGGGGCGTCTGAGGCGTCAGGG30.12992637505413598No Hit
CAAGTTCTTTCCTTCCCTGCCAGCCAGATAGATAGACAGATGGGAAAGGC30.12992637505413598No Hit
GCTGCCCGCTTGCCCGTAGCTGACTCAGCTTCCTCATCTTCATCTCCATC30.12992637505413598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12992637505413598No Hit
CACTTACACTTTATGCACAAAATGTAGGGTTATAATAATGTTAACATGGA30.12992637505413598No Hit
ACTGACTTCTGCTGAGATCGGAAGAGCGTCGTGTAGGGATGTATCCGACC30.12992637505413598Illumina Single End PCR Primer 1 (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12992637505413598No Hit
ATATAATAAGCACCAGAAGCACCCATATCGCCAATCACTGCATAGACTTT30.12992637505413598No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)