FastQCFastQC Report
Tue 10 May 2022
EGAF00003662621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991
Sequences flagged as poor quality0
Sequence length146
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA711.1851109998330829Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT400.6676681689200467Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG210.35052578868302453Clontech Universal Primer Mix Long (96% over 26bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA180.30045067601402103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.30045067601402103No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC170.28375897179101983Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT170.28375897179101983No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC160.2670672675680187No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA150.25037556334501754No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG150.25037556334501754No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA150.25037556334501754No Hit
CGACATACGGGTCCGATTTGCTACCCGAAGACCGCAGGGTCTGTTCATCG140.23368385912201634No Hit
GGCGTACACGATGACCTTCGCCTTCGAGTCATACCGGAAGAACAGCCGGA140.23368385912201634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.23368385912201634No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC130.2169921548990152Clontech SMART CDS Primer II A (96% over 26bp)
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC130.2169921548990152No Hit
TCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATG130.2169921548990152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.2169921548990152No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC120.20030045067601399No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA120.20030045067601399No Hit
GTATCAGCGCATGCCAGTCGTCTGGTGGGTTCCCGCGCCCGAGCATCTTC120.20030045067601399No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC120.20030045067601399No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC120.20030045067601399No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120.20030045067601399Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAAACTGCATGCGACCGCGAGGCGCGCGCAGGAGAACGACCCGACGG110.18360874645301284No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC110.18360874645301284Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.18360874645301284No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG110.18360874645301284Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100.16691704223001166Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC100.16691704223001166No Hit
CTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGACCGCGA100.16691704223001166No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC100.16691704223001166No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT90.15022533800701052Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG90.15022533800701052No Hit
GTACATGGGCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAA90.15022533800701052No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT90.15022533800701052Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA90.15022533800701052No Hit
GTTAAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTC90.15022533800701052No Hit
GTCCCAAGGTGTTGCCCTGGCGGTACTCGTCGCGTGCCGGATCGCCTGGC90.15022533800701052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG90.15022533800701052No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA90.15022533800701052No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA90.15022533800701052No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT90.15022533800701052No Hit
GTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAACGGCATGGCTGG80.13353363378400934No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA80.13353363378400934No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC80.13353363378400934Clontech Universal Primer Mix Long (96% over 26bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC80.13353363378400934No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT80.13353363378400934No Hit
GTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATG80.13353363378400934No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC80.13353363378400934No Hit
GTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTC80.13353363378400934No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC80.13353363378400934No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA80.13353363378400934No Hit
GTACATGGGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTT80.13353363378400934No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC80.13353363378400934No Hit
ATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTA80.13353363378400934No Hit
TTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGT80.13353363378400934No Hit
GTTGCAGAAACTGCATGCGACCGCGAGGCGCGCGCAGGAGAACGACCCGA70.11684192956100817No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA70.11684192956100817No Hit
CCACATACCAAGCGACTTCGCTGGTGCTTCCGCGTTCCTGGATGACTACG70.11684192956100817No Hit
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG70.11684192956100817No Hit
ACGCAGAGTACATGGGCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCC70.11684192956100817No Hit
ACCCAGGCGTACACGATGACCTTCGCCTTCGAGTCATACCGGAAGAACAG70.11684192956100817No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT70.11684192956100817No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC70.11684192956100817No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA70.11684192956100817No Hit
GGCCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGT70.11684192956100817No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT70.11684192956100817No Hit
CTGTTGAGATACTAAAATTATCTGTGGATTGCCGGCATTGAGCCCACGGT70.11684192956100817No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT70.11684192956100817No Hit
ATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCA70.11684192956100817No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT70.11684192956100817Clontech Universal Primer Mix Long (100% over 23bp)
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA70.11684192956100817No Hit
CTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAAGA70.11684192956100817No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG70.11684192956100817No Hit
AATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTG70.11684192956100817No Hit
ATGCTGGACGATCCGCGTCAGTCCGCGGCCTTGGGCCTGCCTCGCCCCAA70.11684192956100817No Hit
GAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTA70.11684192956100817No Hit
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG70.11684192956100817No Hit
TCTCTGGACACTGAGAAGCAAGCTGCTGTTGATTTTTTTCTCGGCTCTCG70.11684192956100817No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC70.11684192956100817No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA70.11684192956100817Clontech Universal Primer Mix Long (95% over 22bp)
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG70.11684192956100817No Hit
GTATCAACGCAGAGTACATGGGCCGGCAAGGCGGAGGAATTGGGGCAAGA70.11684192956100817No Hit
GCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCG70.11684192956100817No Hit
GTCCGATCTTGGCCCTTCGCCAGTGGCGGTGGGAAGGTCCCAAGGTGTTG70.11684192956100817No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA70.11684192956100817No Hit
CTCCAATCCTGCTTGCTTGCCTGTATCAGCGCATGCCAGTCGTCTGGTGG70.11684192956100817No Hit
GTTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCA70.11684192956100817No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT70.11684192956100817No Hit
CTCTTTAATAGCCTTCAGTGACTGTGAGATGCCGTCTACGAGTGGCACGA60.10015022533800699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT60.10015022533800699No Hit
GAGTCATACCGGAAGAACAGCCGGAACCGCCGTCCGATCTTGGCCCTTCG60.10015022533800699No Hit
ATTCTGACCATACATTGGGAATACTCGCCCCAGTAGTTTCTGTTGCCTTA60.10015022533800699No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10015022533800699No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA60.10015022533800699No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT60.10015022533800699No Hit
GCCTGGCACCACATCCAAGATCAACTGGCTCAAGGCCCGGAAGAGCTTGA60.10015022533800699No Hit
CTCATTGGTTTATACTTCAATATAAGTCTTGGTAGGGATAGATAGCCACC60.10015022533800699No Hit
CCTTGGGACCTTCCCACCGCCACTGGCGAAGGGCCAAGATCGGACGGCGG60.10015022533800699No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG60.10015022533800699No Hit
GGACTGCACCATGAACGTCTCGGTGCGGATGTCGTGCTTGGGACCCGCGG60.10015022533800699No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT60.10015022533800699No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG60.10015022533800699No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC60.10015022533800699No Hit
GGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAG60.10015022533800699No Hit
ACACTGAGAAGCAAGCTGCTGTTGATTTTTTTCTCGGCTCTCGGAGAGCG60.10015022533800699No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG60.10015022533800699No Hit
GCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTG60.10015022533800699No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC60.10015022533800699No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC60.10015022533800699Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTATTACGAGGGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGAT60.10015022533800699No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60.10015022533800699Clontech Universal Primer Mix Long (96% over 26bp)
GGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGG60.10015022533800699No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC60.10015022533800699No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG60.10015022533800699No Hit
GGGCAACACCTTGGGACCTTCCCACCGCCACTGGCGAAGGGCCAAGATCG60.10015022533800699No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG60.10015022533800699No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG60.10015022533800699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA60.10015022533800699No Hit
GTACTAGGCCCTTATAGGGGACGAACCCGGGGCTGCGCGTGACGAACCAC60.10015022533800699No Hit
GGCGTGAACTGCACACGCGAATAGCGCAGCGTCATGGCCTGCGCCAGCGC60.10015022533800699No Hit
GCCTGTATCAGCGCATGCCAGTCGTCTGGTGGGTTCCCGCGCCCGAGCAT60.10015022533800699No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG60.10015022533800699No Hit
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC60.10015022533800699No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA60.10015022533800699No Hit
ACGCAGAGTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAACGGCA60.10015022533800699No Hit
AATTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTC60.10015022533800699No Hit
TCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCC60.10015022533800699No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC60.10015022533800699No Hit
GGCCTACACCACATACCAAGCGACTTCGCTGGTGCTTCCGCGTTCCTGGA60.10015022533800699No Hit
ATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAA60.10015022533800699No Hit
GTATCAACGCAGAGTACATGGGGGAATTCACACCGGCGCACGCCACAGGC60.10015022533800699No Hit
TTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAA60.10015022533800699No Hit
TCTCAACAGCGAGTGTATTTAGGACGCACGCAGCCCTATTACGAGGGTTG60.10015022533800699No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA60.10015022533800699No Hit
ATCCAATGGTGGGTGCTAACTCTTTAATAGCCTTCAGTGACTGTGAGATG60.10015022533800699No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)