FastQCFastQC Report
Tue 10 May 2022
EGAF00003662622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5991
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT520.8679686195960608Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA490.8178935069270572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA290.4840594224670339No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.3839091971290269No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG220.3672174929060257Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA200.33383408446002333No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC170.28375897179101983No Hit
GTTCATCGTTGACCCAGGCGTACACGATGACCTTCGCCTTCGAGTCATAC160.2670672675680187No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA160.2670672675680187No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC150.25037556334501754No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC150.25037556334501754No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG140.23368385912201634No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA140.23368385912201634No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG130.2169921548990152No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC130.2169921548990152Clontech Universal Primer Mix Long (96% over 26bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC130.2169921548990152No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA130.2169921548990152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.2169921548990152No Hit
GTTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCA130.2169921548990152No Hit
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG120.20030045067601399No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG120.20030045067601399No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT120.20030045067601399No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT120.20030045067601399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.20030045067601399No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA110.18360874645301284Clontech Universal Primer Mix Long (95% over 21bp)
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC110.18360874645301284No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT110.18360874645301284No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC110.18360874645301284Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG110.18360874645301284Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA100.16691704223001166No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT100.16691704223001166Clontech Universal Primer Mix Long (96% over 26bp)
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT100.16691704223001166No Hit
AATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGAC100.16691704223001166No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG100.16691704223001166No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT100.16691704223001166No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA90.15022533800701052No Hit
GAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCC90.15022533800701052No Hit
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG90.15022533800701052No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG90.15022533800701052No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC90.15022533800701052No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA80.13353363378400934No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC80.13353363378400934Clontech Universal Primer Mix Long (96% over 26bp)
CGACATACGGGTCCGATTTGCTACCCGAAGACCGCAGGGTCTGTTCATCG80.13353363378400934No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA80.13353363378400934No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT80.13353363378400934No Hit
CCCGAAGACCGCAGGGTCTGTTCATCGTTGACCCAGGCGTACACGATGAC80.13353363378400934No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT80.13353363378400934No Hit
GAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTT80.13353363378400934No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA80.13353363378400934No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA80.13353363378400934No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG80.13353363378400934No Hit
GTCCAATGCCAACGTCAAGCTCTTCCGGGCCTTGAGCCAGTTGATCTTGG80.13353363378400934No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG80.13353363378400934No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC80.13353363378400934No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA80.13353363378400934No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70.11684192956100817Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAACGGCATGGCTGG70.11684192956100817No Hit
GTTTTTGATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCT70.11684192956100817No Hit
ATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTT70.11684192956100817No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA70.11684192956100817No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC70.11684192956100817No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG70.11684192956100817No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG70.11684192956100817Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC70.11684192956100817No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC70.11684192956100817No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC70.11684192956100817No Hit
GAGTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAACGGCATGGCT70.11684192956100817No Hit
GCCTGTATCAGCGCATGCCAGTCGTCTGGTGGGTTCCCGCGCCCGAGCAT70.11684192956100817No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC70.11684192956100817Clontech Universal Primer Mix Long (96% over 26bp)
GGTCTGTTCATCGTTGACCCAGGCGTACACGATGACCTTCGCCTTCGAGT70.11684192956100817No Hit
GTACATGGGGTGAGCTGCACGATGGCGTTCTCCTCGCTGTGGCTCGGAAA70.11684192956100817No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT70.11684192956100817No Hit
CGGCTATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTG70.11684192956100817No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA70.11684192956100817No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.11684192956100817Clontech Universal Primer Mix Long (96% over 26bp)
GTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATA70.11684192956100817No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT70.11684192956100817No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG60.10015022533800699No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG60.10015022533800699No Hit
GAGTCATACCGGAAGAACAGCCGGAACCGCCGTCCGATCTTGGCCCTTCG60.10015022533800699No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT60.10015022533800699No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG60.10015022533800699Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG60.10015022533800699Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGATCGACGGGAAAGTGGAACTCTGATGCA60.10015022533800699No Hit
GTATCAGCGCATGCCAGTCGTCTGGTGGGTTCCCGCGCCCGAGCATCTTC60.10015022533800699No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA60.10015022533800699No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG60.10015022533800699No Hit
ATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACT60.10015022533800699No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC60.10015022533800699No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG60.10015022533800699No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC60.10015022533800699Clontech Universal Primer Mix Long (96% over 26bp)
ACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTG60.10015022533800699No Hit
CGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTGCCACGCT60.10015022533800699No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC60.10015022533800699Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT60.10015022533800699No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.10015022533800699Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.10015022533800699Clontech Universal Primer Mix Long (100% over 23bp)
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT60.10015022533800699No Hit
TCTGGTGGGTTCCCGCGCCCGAGCATCTTCTCGAACACGACATACGGGTC60.10015022533800699No Hit
AGTTTGCTCCAATCCTGCTTGCTTGCCTGTATCAGCGCATGCCAGTCGTC60.10015022533800699No Hit
GTGGAACTCTGATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGC60.10015022533800699No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA60.10015022533800699No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC60.10015022533800699No Hit
ACATACGGGTCCGATTTGCTACCCGAAGACCGCAGGGTCTGTTCATCGTT60.10015022533800699No Hit
TTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTA60.10015022533800699No Hit
CCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATA60.10015022533800699No Hit
GTTCCCGCGCCCGAGCATCTTCTCGAACACGACATACGGGTCCGATTTGC60.10015022533800699No Hit
CTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGG60.10015022533800699No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG60.10015022533800699Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGAC60.10015022533800699No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA60.10015022533800699No Hit
ATCCGGCACGCGACGAGTACCGCCAGGGCAACACCTTGGGACCTTCCCAC60.10015022533800699No Hit
ACTATGAACACTCTCATTGTGCTTGAATGGACTAGGGGCTCAAAGGCTCT60.10015022533800699No Hit
GGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATTT60.10015022533800699No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC60.10015022533800699No Hit
GTTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCT60.10015022533800699No Hit
CTCTGGACACTGAGAAGCAAGCTGCTGTTGATTTTTTTCTCGGCTCTCGG60.10015022533800699No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA60.10015022533800699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG60.10015022533800699No Hit
GCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGA60.10015022533800699No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)