FastQCFastQC Report
Tue 10 May 2022
EGAF00003662623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6047
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT600.992227550851662Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA500.826856292376385Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.46303952373077556No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC270.44650239788324786No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC250.4134281461881925No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC230.3803538944931371Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG190.3142053911030263Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.29766826525549855No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC170.2811311394079709Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAACGGCATGGCTGG160.2645940135604432No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC160.2645940135604432Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA150.2480568877129155No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC150.2480568877129155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.2480568877129155No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA150.2480568877129155No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC140.23151976186538778No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA130.2149826360178601No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT130.2149826360178601No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA130.2149826360178601No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC120.1984455101703324No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT120.1984455101703324No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG120.1984455101703324No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT120.1984455101703324No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA120.1984455101703324No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT110.1819083843228047Clontech Universal Primer Mix Long (100% over 23bp)
GGTCTGTTCATCGTTGACCCAGGCGTACACGATGACCTTCGCCTTCGAGT110.1819083843228047No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA110.1819083843228047No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100.165371258475277Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTATTACGAGGGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGAT100.165371258475277No Hit
GAGTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAACGGCATGGCT100.165371258475277No Hit
ACATACGGGTCCGATTTGCTACCCGAAGACCGCAGGGTCTGTTCATCGTT100.165371258475277No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG100.165371258475277No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA90.14883413262774928No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC90.14883413262774928Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCGATCCGGTGGGTCGACCGCCACGCCTGGCGGCCATCTCTG90.14883413262774928No Hit
GTTGCAGAAACTGCATGCGACCGCGAGGCGCGCGCAGGAGAACGACCCGA90.14883413262774928No Hit
CCTTGGGACCTTCCCACCGCCACTGGCGAAGGGCCAAGATCGGACGGCGG90.14883413262774928No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT90.14883413262774928Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG90.14883413262774928No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT90.14883413262774928No Hit
TCTCTGGACACTGAGAAGCAAGCTGCTGTTGATTTTTTTCTCGGCTCTCG90.14883413262774928No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG90.14883413262774928No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG90.14883413262774928Clontech Universal Primer Mix Long (96% over 26bp)
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC90.14883413262774928No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA90.14883413262774928No Hit
GTATCAACGCAGAGTACATGGGATCGACGGGAAAGTGGAACTCTGATGCA80.1322970067802216No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT80.1322970067802216No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA80.1322970067802216No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA80.1322970067802216No Hit
TTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGACCGCGAG80.1322970067802216No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT80.1322970067802216Clontech Universal Primer Mix Long (96% over 26bp)
GCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTC80.1322970067802216No Hit
GTCGTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACT80.1322970067802216No Hit
GTCCCAAGGTGTTGCCCTGGCGGTACTCGTCGCGTGCCGGATCGCCTGGC80.1322970067802216No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC80.1322970067802216No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC80.1322970067802216No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT80.1322970067802216No Hit
CTACTATGAACACTCTCATTGTGCTTGAATGGACTAGGGGCTCAAAGGCT70.11575988093269389No Hit
ATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATG70.11575988093269389No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC70.11575988093269389No Hit
GTATCAGCGCATGCCAGTCGTCTGGTGGGTTCCCGCGCCCGAGCATCTTC70.11575988093269389No Hit
ATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATG70.11575988093269389No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT70.11575988093269389Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA70.11575988093269389No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG70.11575988093269389No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA70.11575988093269389No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.11575988093269389No Hit
GTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAGAATTAAATCC70.11575988093269389No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG70.11575988093269389No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT70.11575988093269389No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT70.11575988093269389No Hit
CCTCGGCACCCTGCGCGTGCACCAGGGCCCGGTGTTCACCAACCTGCTGC70.11575988093269389No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC70.11575988093269389No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA70.11575988093269389No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG70.11575988093269389Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC70.11575988093269389No Hit
AAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGT70.11575988093269389No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC70.11575988093269389No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG70.11575988093269389Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAA70.11575988093269389No Hit
GGCCTACACCACATACCAAGCGACTTCGCTGGTGCTTCCGCGTTCCTGGA70.11575988093269389No Hit
CTCCAATCCTGCTTGCTTGCCTGTATCAGCGCATGCCAGTCGTCTGGTGG70.11575988093269389No Hit
GAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTAC70.11575988093269389No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)