FastQCFastQC Report
Tue 10 May 2022
EGAF00003662624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6047
Sequences flagged as poor quality0
Sequence length146
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT580.9591532991566065Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA540.8930047957664957Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.496113775425831No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA250.4134281461881925No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA240.3968910203406648No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC230.3803538944931371No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC200.330742516950554Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC180.29766826525549855No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC160.2645940135604432No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG160.2645940135604432Clontech Universal Primer Mix Long (96% over 26bp)
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG140.23151976186538778No Hit
GTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAACGGCATGGCTGG140.23151976186538778No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC140.23151976186538778Clontech Universal Primer Mix Long (96% over 26bp)
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT140.23151976186538778No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC140.23151976186538778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.23151976186538778No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA140.23151976186538778No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG130.2149826360178601Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA130.2149826360178601No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG130.2149826360178601Clontech Universal Primer Mix Long (96% over 26bp)
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC120.1984455101703324No Hit
GTTGCAGAAACTGCATGCGACCGCGAGGCGCGCGCAGGAGAACGACCCGA120.1984455101703324No Hit
CTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGACCGCGA120.1984455101703324No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT120.1984455101703324No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG120.1984455101703324No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG110.1819083843228047Clontech Universal Primer Mix Long (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC110.1819083843228047No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT110.1819083843228047No Hit
GTATCAGCGCATGCCAGTCGTCTGGTGGGTTCCCGCGCCCGAGCATCTTC100.165371258475277No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA100.165371258475277No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT100.165371258475277No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC100.165371258475277No Hit
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA100.165371258475277No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT100.165371258475277No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG100.165371258475277No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA90.14883413262774928No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC90.14883413262774928No Hit
GGCGTACACGATGACCTTCGCCTTCGAGTCATACCGGAAGAACAGCCGGA90.14883413262774928No Hit
ATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGC90.14883413262774928No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT90.14883413262774928No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA90.14883413262774928No Hit
GCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATG90.14883413262774928No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC80.1322970067802216Clontech Universal Primer Mix Long (96% over 26bp)
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC80.1322970067802216No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA80.1322970067802216No Hit
GAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTATACAAAC80.1322970067802216No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA80.1322970067802216No Hit
TCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATG80.1322970067802216No Hit
ATTCCAGTTTGCTCCAATCCTGCTTGCTTGCCTGTATCAGCGCATGCCAG80.1322970067802216No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT80.1322970067802216No Hit
GTATCAACGCAGAGTACATGGGCCGGCAAGGCGGAGGAATTGGGGCAAGA80.1322970067802216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.1322970067802216No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG70.11575988093269389Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT70.11575988093269389No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA70.11575988093269389No Hit
GTTCATCGTTGACCCAGGCGTACACGATGACCTTCGCCTTCGAGTCATAC70.11575988093269389No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.11575988093269389No Hit
GAACTGACGCGTGCGGCCCAGTGGCTGCAGACGCTGCGCGCCGAGGTCGG70.11575988093269389No Hit
GTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTA70.11575988093269389No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT70.11575988093269389No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT70.11575988093269389No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC70.11575988093269389No Hit
CTCCGATAGCGACTGGACACAACATGGCAGTTGGCCCACATGGAATATCG70.11575988093269389No Hit
CGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCA70.11575988093269389No Hit
ACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTGGGATAT70.11575988093269389No Hit
GTACATGGGGGTGTGGGCGATGCTGCCGGCGATGGACTGCACCATGAACG70.11575988093269389No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70.11575988093269389Clontech Universal Primer Mix Long (96% over 26bp)
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG70.11575988093269389No Hit
ATCAACGCAGAGTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAAC70.11575988093269389No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA70.11575988093269389No Hit
TCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTG70.11575988093269389No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70.11575988093269389No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC70.11575988093269389Clontech Universal Primer Mix Long (96% over 26bp)
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT70.11575988093269389No Hit
CATGAAATCAACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATA70.11575988093269389No Hit
CCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTG70.11575988093269389No Hit
CCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAGGT70.11575988093269389No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC70.11575988093269389No Hit
TATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGG70.11575988093269389No Hit
CTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGG70.11575988093269389No Hit
TATCAACGCAGAGTACATGGGATCGACGGGAAAGTGGAACTCTGATGCAA70.11575988093269389No Hit
TTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGT70.11575988093269389No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC70.11575988093269389No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA70.11575988093269389No Hit
GTACATGGGCCGTCGGCAACGACTTCCCGCTCGGGATGAAGCTCAACGCA70.11575988093269389No Hit
GTTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCA70.11575988093269389No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)