FastQCFastQC Report
Tue 10 May 2022
EGAF00003662645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99774
Sequences flagged as poor quality0
Sequence length146
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG8550.8569366768897709No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC5600.5612684667348207No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA5540.5552548760198048No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG4610.4620442199370577No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT4470.44801250826868727No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC3910.39188566159520516No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT3210.3217271032533526No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT2890.28965461943993426No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC2750.27562290777156373No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC2730.2736183775332251No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG2700.2706115821757171No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA2660.2666025216990398No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG2560.2565798705073466No Hit
TGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGG2540.25457534026900797No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG2480.24856174955399202No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC2430.24355042395814544No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA2430.24355042395814544No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA2190.21949606109808167No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT2190.21949606109808167No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG2150.21548700062140438No Hit
CTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCC2140.21448473550223507No Hit
GGGGAAGGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCG2090.20947340990638844No Hit
ACCTCATAGAGGGCTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCC1980.19844849359552588No Hit
CACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCT1950.1954416982380179No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG1930.19343716799967928No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC1900.1904303726421713No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC1890.189428107523002No Hit
GCCTGGGCATCCCCGTGAACCACCTCATAGAGGGCTCCCAGAAGTGTGTG1890.189428107523002No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG1880.18842584240383264No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG1860.186421312165494No Hit
CCCTCAACCCGCTGAAGCTCCTGCTGAGCAGCCTGGGCATCCCCGTGAAC1850.18541904704632467No Hit
CCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTG1850.18541904704632467No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG1830.18341451680798604No Hit
GCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGGCCAACCCCCT1800.18040772145047806No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC1760.17639866097380077No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA1700.17038507025878485No Hit
GGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGG1700.17038507025878485No Hit
GTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGG1650.16537374466293822No Hit
GCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACC1640.1643714795437689No Hit
CCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTT1610.16136468418626093No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG1550.155351093471245No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC1530.15334656323290635No Hit
ACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCCTGCTGAG1500.1503397678753984No Hit
GCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGG1490.14933750275622906No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT1480.14833523763705975No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT1410.14131938180287448No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC1400.14031711668370517No Hit
CGTTTATTGAGAGGGGCCGGGGGAAGGGGATGGACGGTCCTCCCCGCGGC1370.1373103213261972No Hit
GGCCGGGGCCGGGACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGA1350.13530579108785856No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT1330.1333012608495199No Hit
TCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCGTGGGGGCCGTGAA1320.1322989957303506No Hit
TCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAG1310.13129673061118127No Hit
GTACATGGGATACTAGCCCACCAGACTCAGAGACGGAACCAGAGACAGGC1290.12929220037284261No Hit
GGCCGGGACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCC1290.12929220037284261No Hit
CCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCA1280.1282899352536733No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA1260.12628540501533467No Hit
GGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGT1250.12528313989616532No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA1240.12428087477699601No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT1180.11826728406198009No Hit
GTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGC1170.11726501894281074No Hit
CCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGC1170.11726501894281074No Hit
CCCACGGCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTAT1130.11325595846613346No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG1130.11325595846613346No Hit
CCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGC1130.11325595846613346No Hit
GGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCCTGCTGAGCAGCC1120.11225369334696414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.11225369334696414No Hit
GTACATGGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATG1110.11125142822779482No Hit
ATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCA1110.11125142822779482No Hit
CTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCA1100.11024916310862551No Hit
TCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGC1090.10924689798945617No Hit
GCCACACACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAG1090.10924689798945617No Hit
CGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCA1080.10824463287028685No Hit
CAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCT1080.10824463287028685No Hit
GTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGC1040.10423557239360956No Hit
GGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGC1000.10022651191693227No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)