FastQCFastQC Report
Tue 10 May 2022
EGAF00003662646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99774
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG7720.7737486719987171No Hit
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG7510.7527011044961613No Hit
CTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTT4810.4820895223204442No Hit
TTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGA4570.4580351594603804No Hit
GCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCC4460.4470102431495179No Hit
GCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCC4400.44099665243450203No Hit
CTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCA3610.3618177080201255No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT3570.3578086475434482No Hit
CTATGAGGTGGTTCACGGGGATGCCCAGGCTGCTCAGCAGGAGCTTCAGC3470.347785996351755No Hit
GTCTTGTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGT3410.34177240563673905No Hit
CCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAG3150.3157135125383367No Hit
TCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTA3080.3086976567041514No Hit
CCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGTCCTGCAGC2970.29767274039328884No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA2930.2936636799166116No Hit
GCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGG2880.2886523543207649No Hit
GCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCC2790.27963196824824105No Hit
ACCTCATAGAGGGCTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCC2620.26259346122236255No Hit
GGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGC2610.2615911961031932No Hit
GTCCTCAGGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGG2440.24455268907731476No Hit
GGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGG2400.24054362860063744No Hit
GCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCCAGCCTGTCGCTGCGCT2370.2375368332431295No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG2250.2255096518130976No Hit
GGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGA2220.22250285645558965No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC1840.18441678192715535No Hit
CTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGG1840.18441678192715535No Hit
ACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCCTGCTGAG1820.18241225168881672No Hit
CCCTCAACCCGCTGAAGCTCCTGCTGAGCAGCCTGGGCATCCCCGTGAAC1820.18241225168881672No Hit
GTACATGGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATG1750.17539639585463146No Hit
GTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTG1720.17238960049712349No Hit
GCCTGGGCATCCCCGTGAACCACCTCATAGAGGGCTCCCAGAAGTGTGTG1720.17238960049712349No Hit
GCGCTGGAGTCGGCGGCGGAGGCCGGGGCCGGGACCCTGGCCAACCCCCT1710.17138733537795417No Hit
GGGGAAGGGGATGGACGGTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCG1670.16737827490127688No Hit
GCCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGG1670.16737827490127688No Hit
ACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTC1590.1593601539479223No Hit
CTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAGCAGGGCCTTC1580.15835788882875299No Hit
TGCCCAGGCTGCTCAGCAGGAGCTTCAGCGGGTTGAGGGTGCCGAGGGGG1550.155351093471245No Hit
CTGTCAGGGCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCC1530.15334656323290635No Hit
TAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTTAATGATGCT1520.15234429811373704No Hit
GCCTGGGGACCCAGCTCAGCCACACACTTCTGGGAGCCCTCTATGAGGTG1480.14833523763705975No Hit
TCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCGTGGGGGCCGTGAA1460.14633070739872112No Hit
GCTTAATGATGCTTTCTCTGGGCTTTTGGGGGAGGGTGTCCACCAGCTTC1430.14332391204121314No Hit
GTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGTCCTCAGGTGTAGATGCT1410.14131938180287448No Hit
CCTGTGGCCCAGCCTGTCGCTGCGCTGGAGTCGGCGGCGGAGGCCGGGGC1400.14031711668370517No Hit
CTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACCCAGCTCAGCCACAC1390.13931485156453585No Hit
GCCCCCAGCAGGGCCTTCAGGGCCTTCACGGCCCCCACGGCCTGGGGACC1310.13129673061118127No Hit
CCTCATGGACACACCCTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCC1300.13029446549201196No Hit
GTCTTGATCAGGGCTGAAAAGTTCCATGGCAGCCTCATAACTGGAGGGTG1300.13029446549201196No Hit
CTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGGCCC1260.12628540501533467No Hit
CACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCT1230.1232786096578267No Hit
GTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGAGGGAG1230.1232786096578267No Hit
GGCTCTGCGTGGCCCTGTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGC1220.12227634453865738No Hit
CTCCAGTTATGAGGCTGCCATGGAACTTTTCAGCCCTGATCAAGACATGA1210.12127407941948803No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT1200.12027181430031872No Hit
GGCTGGAGCAGTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGA1200.12027181430031872No Hit
TTTCAGCCCTGATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGC1160.11626275382364143No Hit
CCCCCGCGCCATGAAGCTCGCCGCCCTCCTGGGGCTCTGCGTGGCCCTGT1160.11626275382364143No Hit
GGCCGGGACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCC1160.11626275382364143No Hit
GGCCAACCCCCTCGGCACCCTCAACCCGCTGAAGCTCCTGCTGAGCAGCC1140.1142582235853028No Hit
CTTCTGCAGAGATCTGCCCGAGCTTTCAGCGTGTCATCGAAACCCTCCTC1110.11125142822779482No Hit
GGCCGGGGCCGGGACCCTGGCCAACCCCCTCGGCACCCTCAACCCGCTGA1110.11125142822779482No Hit
CCTCATAACTGGAGGGTGTGTCCATGAGGAGGGTTTCGATGACACGCTGA1100.11024916310862551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.11024916310862551No Hit
CGTTTATTGAGAGGGGCCGGGGGAAGGGGATGGACGGTCCTCCCCGCGGC1080.10824463287028685No Hit
CCTCATAGAGGGCTCCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCG1070.10724236775111753No Hit
TCTAAATGCTAATTACACAGTGAGCTTTGGGCTATTTTTTCCATGAGCTT1060.1062401026319482No Hit
GATCAAGACATGAGGGAGGCAGGGGCTCAGCTGAAGAAGCTGGTGGACAC1040.10423557239360956No Hit
GGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGA1030.10323330727444024No Hit
GAACCAGAGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTG1010.10122877703610159No Hit
CACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAGATCTGCCCGAGCTTT1010.10122877703610159No Hit
CCCAGAAGTGTGTGGCTGAGCTGGGTCCCCAGGCCGTGGGGGCCGTGAAG1000.10022651191693227No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)