FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003662693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003662693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4133
Sequences flagged as poor quality0
Sequence length146
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG100.24195499637067508No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT70.16936849745947255No Hit
ATATAGTATTTCACAAGTAGTGGAAAACCAGTTACCTCATTGCTTACCAG70.16936849745947255No Hit
GGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCT70.16936849745947255No Hit
GCCAGTGAGCCTCTAATGCTACTGCGCCCTGGGGGCTCCCAGGGCCTGGG70.16936849745947255No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA70.16936849745947255No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC60.14517299782240503No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG60.14517299782240503No Hit
GAATGCAGCCCACGGCGCCATCAATACATGTGCACTGATGTTTACAGTTG60.14517299782240503No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA50.12097749818533754No Hit
AGATAATGCTGTGTTTGCTCTGTGCAGACACAGATGGAAGGGAAGAGGGA50.12097749818533754No Hit
CTTCTGCCTCTCGGCCTCCATCATCTACCCCACCACCTATGTCCAGTTCC50.12097749818533754No Hit
CCTTAAAAGCCCTCTGGTCCTGGAGGCAGGATCATGTGGTGGATTCTTGG50.12097749818533754No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT50.12097749818533754No Hit
GATCCTGATCCAGTGGATCACCACCCAGTGCCGAAAGGATGTGGGCCGGC50.12097749818533754No Hit
AAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCG50.12097749818533754No Hit
GCTGAGAGCAGGGGGAACGCATCCAGGCAGCCAGGGGCTAGGACCTCATG50.12097749818533754No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT50.12097749818533754No Hit
GTCCAGATTGCCTGACACACTGGTAACCAATGACTAGGGGTCAGGTCTCA50.12097749818533754No Hit
ATTCCCACCTCATGAGAATCACATTTCCACATCACAACATGACCAAAGTC50.12097749818533754No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT50.12097749818533754No Hit
GCAGTGCACCCCGGCGGGGACACGGTGGCAATTGGGGGTGTGGACGGCAA50.12097749818533754No Hit
GTATTAAAAAGTTGTTGTATTCAAATTTTGGCAGTGTTTCTATTTACGGT50.12097749818533754No Hit
GAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATC50.12097749818533754No Hit
GGGAAGGAGTCAGCCTCGGGGTAGGTGAAGACGAAGGAAGACGTGTAAGC50.12097749818533754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)