FastQCFastQC Report
Tue 10 May 2022
EGAF00003663925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003663925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5778
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT110.1903772931810315Clontech Universal Primer Mix Long (96% over 26bp)
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA110.1903772931810315No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA80.13845621322256838No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC80.13845621322256838No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA80.13845621322256838No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC80.13845621322256838No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA70.12114918656974732Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTGGACCCAGGATTCACTGGGATCAGCACAGACTTGCTTGCTTCTTT60.10384215991692627No Hit
CCCTGAAGACTTCCTGTCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATAC60.10384215991692627No Hit
TGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA60.10384215991692627No Hit
CTTCTAGAGTATGAGCCGCAATGCACGACTGATACTCGATATGAGCAGCA60.10384215991692627No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG60.10384215991692627No Hit
ACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGT60.10384215991692627No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA60.10384215991692627No Hit
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA60.10384215991692627No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG60.10384215991692627No Hit
GGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCA60.10384215991692627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)