FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003663927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003663927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47.017543859649122Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGGTGAAACCGATGAAGAACATGGCACCGCCCACAACCGTCTTCCACTCG35.263157894736842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT35.263157894736842Clontech Universal Primer Mix Long (96% over 26bp)
CGGCTTCACCGACGACGCGGCCGTGATCGCGGCCCTGGTGGCGACGCTGG23.508771929824561No Hit
GTGGTATCAACGCAGACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23.508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGGGCCCCCAGGACACCAGGCCAGCTGCGTAGAATTTGGTCTTGTCAT23.508771929824561No Hit
GTGCGGGTGGGCGAGAACTCGCCCAGCTTGTGGCCGACCATGGTCTCGGT23.508771929824561No Hit
TTATTGAAATGTTTTCCTTTGTGCTTAACTGGCTGGGCATTCCACAGCAC23.508771929824561No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC23.508771929824561Clontech SMART CDS Primer II A (96% over 26bp)
GTGACCGCCGTGCGCGTGTGCGCGTTGAGCGAGCTCGAGCAGGATGCCGC23.508771929824561No Hit
GTATCGCCCGGCGGATGCATGTCGTGCTCCAACGGGCCTTCCTTCGCGTA23.508771929824561No Hit
GACACAGACAACCGTGAAAGTCACTGGCAACTCAAGCACCGCCCATGTTG11.7543859649122806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAACACA11.7543859649122806No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGATGATAC11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTGGGATTACAGGTGTGAGCCACTGCGGCCGGCCCCCTCTCACCTTT11.7543859649122806No Hit
GTGCGGGTTGTGCACCAGGATGTCGCGGTCCTTGCAGGTGCCAGGCTCGC11.7543859649122806No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
CTCTACTTCATCCATTACTTTCTGGGATTCCTCCACATTCCCTTCAGCTC11.7543859649122806No Hit
GGACTACATTTAGAGAGGAGGGAGGAGAAGTGGCCTGCATTGGGGGAGGG11.7543859649122806No Hit
GTCCTGCCTACCGGCCCTATCAGGCCCGCGTGGTGGCGGTCGAGACTCTC11.7543859649122806No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGCACTCGTTCTCGTACGCTTCGGACTGCGGGAGCAGGCTCGCGGCG11.7543859649122806No Hit
GAGCAAATTTGAGATCTGGTCGTTCTACACAATCTGGGATAGTCATAAAA11.7543859649122806No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GAAGAGCGATCGGGCGGCACAGCTCGATCCGATAAGCGATCGCCTTCTTC11.7543859649122806No Hit
GTGTTGGCGCACGCGGCCTCCAACTTCGGCACCTATCTGTTCCTCACCCA11.7543859649122806No Hit
CTTGTACTTGGGAAGCAGTGCTATCAACGCAGAGTACTTGGGAAGCAGTG11.7543859649122806Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGGCAGTGGTATCTACGCAAAAAAAAAAAAAAAAAAAAAAA11.7543859649122806No Hit
GTCCTGGGGCTCGCTCGCTAGCCTCTGCCCGGCACACATGGACTTGAGCA11.7543859649122806No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
CCGGCGGGGAGGTGACCGACGAGCAGTGGGAGCAGGTCTTCGCGGCCTTC11.7543859649122806No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.7543859649122806Clontech Universal Primer Mix Long (95% over 21bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGACTGTCTCTTATACAC11.7543859649122806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTGATATGTTAAAAGT11.7543859649122806No Hit
GTCGGACACCAAGAGGTTGCCGTTCCAGGCCACATCCTCGGCAAGGTCGG11.7543859649122806No Hit
CAATAGCATGAAGGCTTCCGTTTTCCCTGGAGTCAGTCATGCAAGAAAAC11.7543859649122806No Hit
GAAGTCGTTGAGCGCGAGGAGGCGCGGCAGGAGAAGCTTGCCGAGATCGA11.7543859649122806No Hit
GCCCGGAGGAGGGCGAGGGCGAGTTCTACGCGTGGATGGCGGGGGAGTCC11.7543859649122806No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)