FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003663928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003663928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA915.789473684210526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTTAGCCTAGTTGGCAAGCGAGCAATTTCCACCTCTGTGTGTGTACG35.263157894736842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23.508771929824561Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC23.508771929824561Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23.508771929824561Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTGGTGAGGGTAAGACCAGCGGTGGACGCCACCCGGTCACCCCGTGG23.508771929824561No Hit
GATCGTGCGGTACGCGAAGGAAGGCCCGTTGGAGCACGACATGCATCCGC23.508771929824561No Hit
CGGCGATCGTGTACGCCAGGGTGGACTTGCCCGAGCCGTTCGGGCCCATG23.508771929824561No Hit
GTGCACCGGAGGTGAAGTCGGTGCCACTTCTGCCCTGGCCCCCAAGATCG23.508771929824561No Hit
ACCTGGGACTGATCTCAGGCTGGGGCCGAACAGAGAAGAGAGATCGTGCT23.508771929824561No Hit
GTAGTTGCGCTCGCGCCGGGCGCCGGCGCCGCCGTGCAGGATCAGGGCGG23.508771929824561No Hit
CGCCAGTCCCGCCGGCGCCTACTTCACCGGCGGCGTGCAGCCGGTCTCGA11.7543859649122806No Hit
GCGAGACCGGACGCGCGCCGATCATCAGCGAGACGATCGTCATCGCGGCG11.7543859649122806No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA11.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CCTGGAGAGTCTTCTCGATGTTCTTGGCCCGCTTCTTGGGGCGCTGGGGC11.7543859649122806No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC11.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATGGCGTCGACGATCTCCTCGGCGGCGGCGACCGGCGTGACCGGATCC11.7543859649122806No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAAAAAAGCTTACC11.7543859649122806No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACCACTGCTTCCCATGTACT11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
AATGACCACAGATTTAAAGTGCAAACTCATGAAAACATTAAAGTTGCTTA11.7543859649122806No Hit
GGTTCAAGGTGAACTACATGACAGACAGTATGGCAATCCTGTGCGTTTGG11.7543859649122806No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11.7543859649122806Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGTAGGTGCGGAGGGGGTTCCGGTCCGCGGCGGGCAGCGCCCGCCACCG11.7543859649122806No Hit
GAGCGACACCGCGGCCACCTTCACGAGGATCTGGTTCGCGCCCGGTGCGG11.7543859649122806No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTTATACAC11.7543859649122806No Hit
CGTGATCGTCGCGGAGGACATCGGCAAACTGCCGGATCGCACAGTTTCGG11.7543859649122806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAAAACA11.7543859649122806No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAGTGAA11.7543859649122806No Hit
ATTGTGTCGAGGCGTTCAAGCACGGCCTTCCGCTCCATGGGCGTGCGGAC11.7543859649122806No Hit
TTCCCCTGCTGGACTCCATCACCACACTCCCCCCAGCCTTCACCTGGCCA11.7543859649122806No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.7543859649122806Clontech Universal Primer Mix Long (96% over 26bp)
AGCTGGCGCTGCGGCGGAAGAAGGTGCTGAGCACCGAGGAGATGGAGCTG11.7543859649122806No Hit
TACCGAGCGTGTTGAAGATTTTACGGACGACTGTTTTAGTGAGCCCGCGC11.7543859649122806No Hit
CCTTTCACGCTTGTTGTCGCGATTCCGTTTTGATCGGTCACCGCTGTTAA11.7543859649122806No Hit
ATCAAGGACTACGAGGCACGCGGCGGCTATCAGGCGCTGCGCAAGATCCT11.7543859649122806No Hit
GTGTTCATGAGTTAAATGAAGAAATTGGTAAATTGTTAGCCAAGGTGGAA11.7543859649122806No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)