FastQCFastQC Report
Tue 10 May 2022
EGAF00003664005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19541
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.3070467222762397No Hit
GTGTAGGGCATCGTCGGGTTGGTCGACGACGGCAGGAAGTTCTCCTCGCC320.16375825188066118No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG300.15352336113811985No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC300.15352336113811985No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA290.14840591576684917No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA290.14840591576684917No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCC270.13817102502430786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.1330535796530372No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT250.12793613428176653No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT250.12793613428176653No Hit
GTAATGGACACCGACCGAGCCGCCGATGTCGAGCACCTTGGTGGCACCGT240.12281868891049587No Hit
GTGCTGACCAACGCCCTCATCCTCGACCACTGGGGCATCGTGAAGGCCGA230.11770124353922523No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC230.11770124353922523No Hit
CGCTAACACCACACGCAAGCTGGTCGGCATCGCTCATCAAGCGGACGGAG230.11770124353922523No Hit
GATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGG220.11258379816795455No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT210.10746635279668389No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC210.10746635279668389No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT210.10746635279668389No Hit
GTACATGGGAACAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGA200.10234890742541324No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG200.10234890742541324No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC200.10234890742541324No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG200.10234890742541324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)