FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003664017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCTTCCTTTCCAACTTGGAAGCTGCAGAATGGCTCCCGCAAAGAAGGG37.6923076923076925No Hit
CCTGACAGCTGACTCCAGCGGCGGCAGCACTCCCGACCGCGGCCATGACT25.128205128205128No Hit
ACCTACTGCAATCCTGTATCGCCAAGTGAAGCAACTGTCTCTTATACACA25.128205128205128No Hit
GAAATAGGTGAGGTGTCTTTCCCACATCAGAGGCACCTGGGATCCTTGTA25.128205128205128No Hit
CCTCCCACCCCCTTGCTTCTGCTTTCACCATGTGAAGTACCTGCTCCTGC12.564102564102564No Hit
GAGTCAATTCAGGGTGGTGAATATGAAACCAGTAACGTTATACGATGTCG12.564102564102564No Hit
GGTATTAACTTTTCGTTTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTT12.564102564102564No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACCAAGCAGACATCTCGTA12.564102564102564No Hit
CNTGTCTCCGGTCAATAACCAATAGCGGAACCTGGATGCTCATATTGGCT12.564102564102564No Hit
TCCCCAAAGAGATGACATTCCACAGGATTCCTGTCTGAGAAGAGCATGGG12.564102564102564No Hit
CACTGTGAGTGCGATTAAATGTCTTTCTAATGATGCCATCCAGGCCCCAA12.564102564102564No Hit
AAGTAACAAAGAACTGCGCTGAAGGATGGTCCTTTAACCATAGTCTCACC12.564102564102564No Hit
CCTGAATACAATACACTGATGGGTCTTGACGCTTTATCCCTGTCTCTTAT12.564102564102564No Hit
GAACGGCGATGTCCCACACCAAAAGCAGGGCCCCGGTGTGCATCCAGATC12.564102564102564No Hit
GAGTCGGAATCCGATGAATCCCTTGTCACAGACAGAGAGTTGCAGGATGC12.564102564102564No Hit
CCCACAAAGTGCGGGTTCCCCTGGAAAGTGAGTACCACTTGTCCCTCAAA12.564102564102564No Hit
GTCCCGCATCTAGCCTGCTGCACGCTGTGTGAACGTAACCGCGAGCACCT12.564102564102564No Hit
GTCCTTGGCCGCTTTGATCTGGAGCCACGTGTCACCCAGAGCTTTGGCAA12.564102564102564No Hit
GGGATGGGCATCGTGATGATCTGCATGTTCACCTGTCTCTTATACACATC12.564102564102564No Hit
GGAACTGCGTGGGGTCGTCCGGCAGGCCGGGGGTCGTCTCCGGCTTCTCC12.564102564102564No Hit
CCTCTGCAGCACGCCGCGTTCACAGGGCTGTGGTGGCTCTGTCTCTTATA12.564102564102564No Hit
TGACAGTCTGGGTTTGAACCAGGCTTTCTGGCTACACACTCAGTAAACCA12.564102564102564No Hit
ATACTGCAAGGTGTTGGAGATGAGCCCCCACTCTTTGGCTGTCTCTTATA12.564102564102564No Hit
GAGAAGAACTGGTTCCACTACGCTGCCCGGATCTGGGATGGGGTGAGAAA12.564102564102564No Hit
CTATCTTAGGGTTCTCCGGAGGGGCAGGCCTAATCTGTCTCTTATACACA12.564102564102564No Hit
AGTTTTGCTGTGCAACACATGCTGCTCTATTTTGGTCTCGAGTTGGACAA12.564102564102564No Hit
CTCAACATCCTGTGTTCAACTGATCCTCCCACCTCAGCCTGTCTCTTATA12.564102564102564No Hit
GTACACATAAATGAAAATTTCGTCAAATTGGCAGAAGCCCTCTACATTGC12.564102564102564No Hit
ATTGCTGCCATCTATTGGTTGCTCATGTTAGTGGGACGTTCTGCAAGTCT12.564102564102564No Hit
GTCCGGGAGAACACGGCTTCCACGGGTAAGTCAAAGACCTGTCTCTTATA12.564102564102564No Hit
GGGTGCCGATGACGTCGCCTCCGACAAAGGCATAACGACCCTCGTACCTG12.564102564102564No Hit
GCGCGACGCCCACCTCGGTGTTTGTCGCCTCCTCAATCCTGTCTCTTATA12.564102564102564No Hit
CCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC12.564102564102564No Hit
GCACAGATCGACAATGGGTTAAAGACTCTTGACGCCGGCGTCACCGAGAT12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)