FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003664018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTTGAGTGCCCGAGGTGCACGCTTCTTGAAGCCCACTCCATGGATGC37.6923076923076925No Hit
CGCCTGCACCCTCAGGTCGGTCAGCGCTGCCTCCAGCAGCACGTACTCTC25.128205128205128No Hit
TTGCTTCACTTGGCGATACAGGATTGCAGTAGGTCTGTCTCTTATACACA25.128205128205128No Hit
CTTTCAAACTTTAATGAGCCTCCCAATCACCAAAGGAACTTGTTAAATGC25.128205128205128No Hit
GTCTTTGACTTACCCGTGGAAGCCGTGTTCTCCCGGACCTGTCTCTTATA12.564102564102564No Hit
GGAAATAACCCGTTGCTTGCGCCCTGCGGTGGTGTTAGCGACGCCAGCTC12.564102564102564No Hit
CTACTGCTTTGATCTGGATGCACACCGGGGCCCTGCTTTTGGTGTGGGAC12.564102564102564No Hit
CCAAAGAGTGGGGGCTCATCTCCAACACCTTGCAGTATCTGTCTCTTATA12.564102564102564No Hit
GCTAAGAGCATCGAGGGGGCGCCAAGAGGCAAGGGGCGGGGACGGGCGGT12.564102564102564No Hit
ATCTGGCCGTGGGGAAGCTGGTTGTGCGGATGACGTTCACTGAGCTGTGC12.564102564102564No Hit
TCTTCGGCCCCTCTAGCACGACATTGAGGCCTGGCTTCAGAAGCCCTCGG12.564102564102564No Hit
GTGGTTTACTGAGTGTGTAGCCAGAAAGCCTGGTTCAAACCCAGACTGTC12.564102564102564No Hit
ACTTGCAGAACGTCCCACTAACATGAGCAACCAATAGATGGCAGCAATCT12.564102564102564No Hit
GTGAACATGCAGATCATCACGATGCCCATCCCCTGTCTCTTATACACATC12.564102564102564No Hit
GATTGAGGAGGCGACAAACACCGAGGTGGGCGTCGCGCCTGTCTCTTATA12.564102564102564No Hit
AGGTGCTCGCGGTTACGTTCACACAGCGTGCAGCAGGCTAGATGCGGGAC12.564102564102564No Hit
TCACACCACTGCACTCCAGCCTGGGCAACAGAGCGAGACTTCGTCTCAAA12.564102564102564No Hit
AGCAGAAGCTTTGAGGGACAAGTGGTACTCACTTTCCAGGGGAACCCGCA12.564102564102564No Hit
ATAGCAGCGTCTGCTTCTGGGTAGGCCTCAGGGAGCTTTTACTCATGACA12.564102564102564No Hit
ATTAGGCCTGCCCCTCCGGAGAACCCTAAGATAGCTGTCTCTTATACACA12.564102564102564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12.564102564102564No Hit
GGATAAAGCGTCAAGACCCATCAGTGTATTGTATTCAGGCTGTCTCTTAT12.564102564102564No Hit
CAGCAATGTAGAGGGCTTCTGCCAATTTGACGAAATTTTCATTTATGTGT12.564102564102564No Hit
GCTGAGGTGGGAGGATCAGTTGAACACAGGATGTTGAGCTGTCTCTTATA12.564102564102564No Hit
NGTATTGAGATACCGACGATCAAATCTCGGGCTAGTAACATACCGATGAC12.564102564102564No Hit
GCTTAGTTTGTCCAACTCGAGACCAAAATAGAGCAGCATGTGTTGCACAG12.564102564102564No Hit
AGCCACCACAGCCCTGTGAACGCGGCGTGCTGCAGAGGCTGTCTCTTATA12.564102564102564No Hit
GCTCTGGTGCATTTGTTTGCCCTACGATGGGTGCTTGGGGCCTGGATGGC12.564102564102564No Hit
GACGCGGATCTCGGCGTTCGTGGAGAAGCCGGAGACGACCCCCGGCCTGC12.564102564102564No Hit
GGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCG12.564102564102564No Hit
CTACTTTCTCACCCCATCCCAGATCCGGGCAGCGTAGTGGAACCAGTTCT12.564102564102564No Hit
CACCCACGCGCGTCTGAGTAACGGCGCTCGACGCCTTTGCTGCCGCTGAG12.564102564102564No Hit
GCAGAGTACATGGGACCAGACATGGAATCCTCTTTACCTGGCTGTGGGCA12.564102564102564No Hit
GTGGAGGAGCATTTCCGCAGGAGCCTGGGCAAGAATTACAAGGAGCCCGA12.564102564102564No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)