FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003664083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGGACACTCTCCATGTCGTAGCTTTCCTCTAGTTTAAACCGCGGGA37.317073170731707No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24.878048780487805Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG24.878048780487805Clontech Universal Primer Mix Long (96% over 26bp)
CTATGTACTGGATTTGGATGTGGATTTTGAAAGTCAAGTCATTGAGGGGA24.878048780487805No Hit
GGGAGTTGCCGAGCACCGCGAACGGCCGCTCGCCGACCTCCGCGCGGATC24.878048780487805No Hit
GGTGCAGGGCGCCGCCTCAGGCGGTCAGGGCCTCGCGCATGACGTCCAGG12.4390243902439024No Hit
TTTATCACACTGGATTTAAGTGTTCTTATATGGAAACACACACAAAAATC12.4390243902439024No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAATTACTCC12.4390243902439024Clontech Universal Primer Mix Long (95% over 22bp)
GCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12.4390243902439024No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GGCACCTGGCTTGCAGATCAAGCTAAGTAATATTTGTTAAAGAACAAATA12.4390243902439024No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT12.4390243902439024Clontech Universal Primer Mix Long (100% over 23bp)
CTGTTAATGTCATTTCAAAAGCTGCATTCACTGATTCTTTGGCCATCGCT12.4390243902439024No Hit
ATCAACGCAGAGTACATGGGACAGTGGTATCAACGCAGAGTACATGGGAA12.4390243902439024Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTGTGACTCGCTCCCGGGCATGCTTCTCACAGTAGATTTGATCCTCCACA12.4390243902439024No Hit
GATTCAGTGCGCGAGCCACTTGATGTTGATGCTGGCGCTGCCCGTCGAGG12.4390243902439024No Hit
CACTTAACTACTCTGTTTAATTCTCTTGGATCTTGGAATATAGAATAATG12.4390243902439024No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAAC12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCTGT12.4390243902439024Clontech Universal Primer Mix Long (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA12.4390243902439024Clontech Universal Primer Mix Long (95% over 22bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAGGACTGGCGGGATGGGCTCAGCCTGTA12.4390243902439024No Hit
ACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAAT12.4390243902439024No Hit
CGCTGCTCCCTCTCGGATGACCCGTCCTTCTCCGAGCTGCTGACGCGCAC12.4390243902439024No Hit
AATTTAGCAAATGCACCACCAATAAACCCCGAGCCATCAAGCTCTACGAA12.4390243902439024No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.4390243902439024No Hit
GAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTACAACAG12.4390243902439024No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12.4390243902439024No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC12.4390243902439024Clontech Universal Primer Mix Long (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGAC12.4390243902439024No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTAAATTGGAAGCAGTGGTATC12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTGGTTGTTACCCCGG12.4390243902439024No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)