FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003664084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAAAGTCAGCAAGAATGAGGAGAAACCTGTGCAAATGATGTTTAAGC37.317073170731707No Hit
GAGCTAGAAATCCCAGAAGCATGTTCCTGGTTGTCATGGTTACCATCTTT24.878048780487805No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24.878048780487805Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCGAGAACCCCCACGTCGTCCGCCGCGGCTCCGGCGTCCCGCTGATC24.878048780487805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTGGGTTGATACCACTGGTTCCCC12.4390243902439024No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12.4390243902439024No Hit
GAACACAGCCCTCAGGTTCTTCTCTTGTCTATTCTGGTGTCTTGAATGCC12.4390243902439024No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGT12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGT12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT12.4390243902439024No Hit
GGAGGGCGTGGGCGAGGGCGTGTGGGATGAGGGCTCGGCCTGGGCTTTCC12.4390243902439024No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GCTCAGAAGTTGCCTATGTGTGACAAATGTGGCACTGGGATTGTTGGTGT12.4390243902439024No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA12.4390243902439024Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGCTTCCCATGTACTCTGCGTTG12.4390243902439024No Hit
GTCAATGGCTATGCCTTTGGCGGGGGCTGTGAGCTTGCCATGATGTGTGA12.4390243902439024No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAT12.4390243902439024Clontech Universal Primer Mix Long (95% over 21bp)
GTTGATTTTCTAGCAGGCGTAACTCACGGTAGGCAATAATCAAGAAATTA12.4390243902439024No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT12.4390243902439024No Hit
TCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCT12.4390243902439024No Hit
GTACTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA12.4390243902439024Clontech Universal Primer Mix Long (95% over 21bp)
ACATCAAGGTCCGCAAAGCGCGCAGTCTGGGGCTCACCTCCCGGCAGAAG12.4390243902439024No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAA12.4390243902439024Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CATTCATATAAAGCAGGCAATGTAACCATTTCTTCAGGTTTTCTTAAGAG12.4390243902439024No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12.4390243902439024Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA12.4390243902439024Clontech Universal Primer Mix Long (95% over 22bp)
CTTTGTTAGTTACTCAGCCAACAGATCTCAAAGGATCCCAAAAGATCCCC12.4390243902439024No Hit
GAGAGGGACGGTGGCTGCAGAGGCGCGTTCTGCAGGGCGAACATCACCTG12.4390243902439024No Hit
ATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT12.4390243902439024No Hit
CATAGGCCCCCATCCTAAGCGAGCTGGAGGAGGTTTGCAGGACAGAGATG12.4390243902439024No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)