FastQCFastQC Report
Tue 10 May 2022
EGAF00003664114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215994
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG37390.3074850698276472No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT36650.30139951348444155No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG26030.21406355623465248No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC24010.19745163216265868No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT19770.16258303906104798No Hit
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG19770.16258303906104798No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC19670.16176066658223642No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC19220.15805999042758434No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC18340.1508231126140425No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG17150.1410368801161848No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG16120.13256644358442557No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT14740.12121770337682586No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC14670.12064204264165777No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA14520.11940848392344042No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT14400.11842163694886652No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA14300.11759926447005495No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA14230.11702360373488684No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC14150.11636570575183759No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG13730.11291174134082899No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC13720.11282950409294781No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC13710.11274726684506668No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG13630.11208936886201741No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT13420.1103623866565131No Hit
CCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTC13280.1092110651861769No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC13040.10723737123702914No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC12890.10600381251881177No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA12820.10542815178364368No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA12650.10403011856966399No Hit
TAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAG12600.1036189323302582No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT12550.10320774609085243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)