FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003664151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGGAAACGGTGTGTACATTTTTGTACTTAAAGGAGAGGTTAATGTAAA512.195121951219512No Hit
CTGCAGGGCTCCCGCGGGCGCCAGCCGGTCGATATCGAGGCGATCATCGA37.317073170731707No Hit
CCCGGCTCAGGTGCAGTAAGTGTCGTCAGTGCCCTTGCTCATGAGTGCAA24.878048780487805No Hit
GGTGCGTGGCGTTGCGCCCGGCGAACGACTCGAGCAGCCAGCCCTTGATG24.878048780487805No Hit
GATGATGAACCGGCCCGCAGGCGGGCGCGCGGGAGCGAAGCCTTCTTCGG24.878048780487805No Hit
CTGCTTGCCCTGTGCGCCGCGCTGTCGATCTCGGCAGCGATCACCATTTC12.4390243902439024No Hit
TCCCATACCAGAGCAGGCGAGGCGAACGGCTCCGCCCGACCGGTGACCGC12.4390243902439024No Hit
CAGATATAGTTGTTCAGAACTTCTCCCAGCAGGTACAGGAAGTTCATTAA12.4390243902439024No Hit
GCTTCCCACTCAGAGGCTTACAACAACTGTTGATATGTTCATAATACTGT12.4390243902439024No Hit
ATGTACGATGACATTGCTTACTCTGAAGACAATCCCACTCCAGGAATTGT12.4390243902439024No Hit
TCGTAGAACAATTCCAGCCGGCGCGGGTCGAGGTTCTTCATCCGCGGCAG12.4390243902439024No Hit
GCCCAGAGGATCGATCCGGCCCGGCGCGACCATTCGGCCCAGACCGAAAG12.4390243902439024No Hit
GCCCGGAACCGCGTCGACCTTGATGCCGAAGGCCGGCATGGCGAAGGCAT12.4390243902439024No Hit
GCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTA12.4390243902439024No Hit
AGATTCAAGATTCCGAGAGTCTTCTTGATAATTTTGTCCAGCTGGATAAA12.4390243902439024No Hit
GAAATGGGCTTCGTCGATACGCTCGACCGGATCATCTACGACGAGCTGAT12.4390243902439024No Hit
TCTCTTGGATGATGATGAATCAGAAGGAGAAGAATTCACAGTCCGAGATG12.4390243902439024No Hit
GGGGCCTTACCGCCCTCCATACCTCTCGAGCTGGTTCCCCGCCACGTGGC12.4390243902439024No Hit
GCTGCGTGCATGCTGCAAAAGCATCAGTGCCACGAGGCCCATGACCTCCG12.4390243902439024No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCCTAATACGATCTCGGTTG12.4390243902439024No Hit
GCCCTGGCGCATGTCGCGGCGGTCGGTTCGACGCAGGTCGAACAACGTAT12.4390243902439024No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTTGGACTTTAATCGTTGAACA12.4390243902439024No Hit
CAACCCGCACTGGCTGGGCGCGCACCCCGTCTACACGGTGTCCAAGCTGT12.4390243902439024No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG12.4390243902439024No Hit
ACCTCATCAACCTGCTCGAGCTCCCGCACGACGAGGGTGAGAGCGAAGTC12.4390243902439024No Hit
AGCCGGAGCGGGACGCCTCAAACCTCATGGGGAGCCGATGACTGTAATAT12.4390243902439024No Hit
CGCGCGAGCCGCACCGTTACCTCACGAAGATCGGGTTCAACCAGATCGCC12.4390243902439024No Hit
GCTCTGCCGCGGGGTGAAGCCGATGATCTCTCGCGTGAAGCGGGTGAAGG12.4390243902439024No Hit
GGTCTGCATGGACTATCGCGTCCGCCATGGCATCCGCCTGCGGTCCGCCG12.4390243902439024No Hit
GTCATAGCCCAGTGCGACGAAAAGTAGTGGGCCAAGGTAGATCGGCAGAA12.4390243902439024No Hit
GTCCCGACCTTCGCGTCGATGCCAAGGCTTTCCAAGACGAGCGCCAGATC12.4390243902439024No Hit
GCAATGAGGGCTTCGCGGCAACGATCAACCCGGCGCTCGCGAGCAACTAT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)