FastQCFastQC Report
Tue 10 May 2022
EGAF00003664163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417811
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66010.4655768646173573No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT62530.4410319852222898No Hit
GCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC48380.3412302486015414No Hit
CTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCT43310.30547089844838277No Hit
GTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGGCCTGGATCCG38580.2721096112246273No Hit
CTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCC36430.2569453897592838No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCT35630.25130288874892354No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT33750.23804301137457673No Hit
CATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTG33290.23479857329361953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30680.21638991374731895No Hit
GTCATACAGTTGACAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGG29770.20997156884803403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29580.20863147485807348No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28640.20200153617090008No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28550.2013667548072345No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC27980.1973464728373528No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26950.19008175278651385No Hit
CCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACCAG25100.17703346920005558No Hit
GTCTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGG23980.16913396778555112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT23740.167441217482443No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22840.16109340384578763No Hit
AGTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTTGAGTG22690.16003543490634506No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22270.15707312187590589No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21900.15446346515861423No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGT21760.15347602748180117No Hit
CATCTGCACTGTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGG21490.15157168339080457No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21000.14811565152195885No Hit
GTATTAACAAGAATTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCT20610.1453649322794082No Hit
CTCCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACC20540.14487121344100165No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20250.14282580682474605No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19690.13887605611749382No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19540.13781808717805122No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19450.1371833058143857No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19300.13612533687494313No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG19190.1353494929860186No Hit
GTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCTGG18880.13316302384450396No Hit
ACCTTCTACAGTCCGTCCCTCAGGGGTCGCGTCACCATATCCGTGGACAC18210.12843742924832718No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18140.12794371040992064No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT18080.1275205228341436No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17770.12533405369262898No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC17710.12491086611685198No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGT17350.12237174066218981No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGCTCAGTCAGA17230.12152536551063575No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16680.11764614606601302No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16600.11708189596497699No Hit
ATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGG16300.11496595808609188No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGCTG15780.11129833242935765No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15760.11115726990409865No Hit
CTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTT15570.10981717591413806No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15390.10854761318680697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15360.10833601939891847No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15340.10819495687365947No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTCTA15300.10791283182314144No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15170.10699592540895789No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15060.10622008152003336No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG15000.10579689394425633No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT14900.10509158131796127No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14890.10502105005533177No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14780.10424520616640721No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14770.10417467490377771No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14670.10346936227748268No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14670.10346936227748268No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14660.10339883101485318No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14480.1021292682875221No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14370.10135342439859754No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14350.10121236187333854No Hit
TCCCAGAACAAATTCTCCCTGAAGGTGACCTCTGTGACCGCCGCGGACAC14330.10107129934807954No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA14260.10057758050967301No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14230.10036598672178448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)