FastQCFastQC Report
Tue 10 May 2022
EGAF00003664164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417811
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC69750.4919555568407919No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT65940.46508314577895077No Hit
GCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC48280.34052493597524636No Hit
CTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCT44210.31181871208503814No Hit
GTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGGCCTGGATCCG37800.2666081727395259No Hit
CTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCC36100.25461785809251025No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCT35260.24869323203163185No Hit
CATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTG33630.23719663622302267No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT33550.23663238612198662No Hit
GTCATACAGTTGACAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGG30170.2127928193532142No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30010.21166431915114214No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG29780.21004210011066354No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28880.20369428647400817No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC28810.20320056763560163No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28080.19805178546364785No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26850.18937644016021882No Hit
CCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACCAG25540.18013684475575378No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT23670.16694749864403646No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23580.16631271728037095No Hit
GTCTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGG23110.16299774793678423No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22910.16158712268419415No Hit
AGTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTTGAGTG22900.16151659142156466No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22550.159047997229532No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21680.15291177738076514No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21390.1508663707645095No Hit
GTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCTGG21070.1486093703603654No Hit
CATCTGCACTGTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGG20900.1474103388956638No Hit
GTATTAACAAGAATTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCT20650.14564705732992622No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGT20540.14487121344100165No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20280.14303740061263454No Hit
CTCCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACC20180.1423320879863395No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19770.13944030621852985No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19550.13788861844068076No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG19530.13774755591542173No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC18860.13302196131924493No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA18380.12963646071302873No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT18250.12871955429884518No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18200.12836689798569767No Hit
ACCTTCTACAGTCCGTCCCTCAGGGGTCGCGTCACCATATCCGTGGACAC18020.12709733525836658No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17870.12603936631892404No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17650.12448767854107495No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGT17260.12173695929852428No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGCTCAGTCAGA17020.12004420899541617No Hit
ATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGG16530.11658817712657046No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT16380.1155302081871279No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16280.11482489556083285No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16260.11468383303557385No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC16260.11468383303557385No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA16250.11461330177294435No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16240.11454277051031485No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGCTG16100.11355533283350179No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG16010.11292055146983623No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15980.11270895768194773No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15970.11263842641931823No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15960.11256789515668873No Hit
CTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTT15850.11179205126776418No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15820.11158045747987567No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15630.11024036348991509No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTCTA15620.11016983222728559No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15580.10988770717676756No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15520.10946451960099053No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15520.10946451960099053No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG14940.1053737063684793No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC14890.10502105005533177No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14870.10487998753007277No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14870.10487998753007277No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14680.10353989354011219No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14520.10241139333804013No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA14490.1021997995501516No Hit
CTCCCAGGCTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCA14370.10135342439859754No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14250.10050704924704351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)