FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003664279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAGCTCCTGCTGGATAATTCGCAAACATAGTCATGACAATTAATAGC22.898550724637681No Hit
GGATGATAGACCTACTGGAGGAATACATGGTTTACAGGAAGCATACCTAC22.898550724637681No Hit
GTTCTTCGCGGGCTTCATCGCGGGACGGCGCGAGCTGTTCGCCAATGCCG22.898550724637681No Hit
GGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC22.898550724637681No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACA22.898550724637681No Hit
GTATAACATGGACTCTTTTTCAAGCGGAAACTCTACTTCCTTTAAAAGAA22.898550724637681No Hit
CGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGT22.898550724637681No Hit
GACTTTCCCCATCAGATGGTCCATCTCGTGCTGGACGCACACCGCCAGCA22.898550724637681No Hit
GATGGAGAGTACTGGCTGGGGCTGCAGAACATGCACCTCCTGACACTGAA11.4492753623188406No Hit
TGCCATCAGCTGGTCGAGGTCGAATGGCTTGGTGATGAAGTCCGCGGCGC11.4492753623188406No Hit
CTCTCTCTGGATGGTGGCCTTTATCTCTAGCCTATGCCTTCCTTATTGGG11.4492753623188406No Hit
TTGTAGTTCAACTGTCTCTGGTAACCAGTGTTGAGATAGCCAATGGCCTT11.4492753623188406No Hit
CTCCTGGGCTCAAGAGATCCTCCCACCTCAGCCTCCTGAGTAGCTGGAAC11.4492753623188406No Hit
ATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGA11.4492753623188406No Hit
ATACAACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATCTG11.4492753623188406Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAGTGGGGGG11.4492753623188406No Hit
TTTTTTTAGCGTTGATACCAGAAGCAGTGGTATCAACGCTAAAAAAAAAA11.4492753623188406No Hit
GATTACAGGCATGCACCAACACACCTGAATAATTTTGTATTTTTAGTAGA11.4492753623188406No Hit
TTATAAAATTTGCCAAATAAACATGTCAAAAACAAACTTAAAAACAAAGT11.4492753623188406No Hit
GGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATCATCAA11.4492753623188406No Hit
GAAATAAAGATCTTCCCAGATAAAGCAAAGTTGGGAGATTTCATCAACAC11.4492753623188406No Hit
GAGCAGGCCTAAGAAGAAAATGCTCTGTGCTAGCTCTGTGTGTGTGTGTG11.4492753623188406No Hit
CTCCAGTTACGGTGCTGGCCGTGGAGTCTGGGTTCACACCGGTGCCCGCG11.4492753623188406No Hit
GTGTTAATCAAGTCAGTAGGGGGGAAATCGAGCTACCATACAAAATAATT11.4492753623188406No Hit
GCTAGGAACCGCAAGCAAATTAGCTCAAATTCAGTGAGCTTCGACCTTTC11.4492753623188406No Hit
TATCTCATTTTATAGACTCCATTGACAATGGTCCAACCGAAAAGAAAAAT11.4492753623188406No Hit
GGGGCGAAGCTACCATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGA11.4492753623188406No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC11.4492753623188406No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC11.4492753623188406No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGT11.4492753623188406No Hit
GGTGACTGCCACGCTGATGCTGAGTGGAAGCCAAGCCCCTTCAGTCAGAG11.4492753623188406No Hit
TTCCATTCCATTGCAATCGAGTTGATTCCATTGCTTTGCATTCCATTCCA11.4492753623188406No Hit
GACACTGAGTATGCTGAGTGCAACTTAGCTTAGGTCACCAGTATCTCCTA11.4492753623188406No Hit
AAACTAAGTAAATTGTTAAAATTTTGTTAAAACTGAGAACTATTTGCCTT11.4492753623188406No Hit
TCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGTGGT11.4492753623188406No Hit
GTTGCAAATTTTTTATGTCAACTGCCCCACGGAAAGCTTTCCTTGGGATT11.4492753623188406No Hit
GTATCAACGCAGAGTACATGGGAGCCCGAGTCCCGTCCTCAGGCTTCCGG11.4492753623188406No Hit
CATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGC11.4492753623188406No Hit
CCGCAGGGTCGATCCGGTGACGGTCAGGCGTTTGGCCATGATCTGGGCGA11.4492753623188406No Hit
TCGAGAAAGAGCTATCAATCTGTCAATCCTGTCCGTGTCCGGGCCGGGTG11.4492753623188406No Hit
CAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTG11.4492753623188406No Hit
TTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATA11.4492753623188406No Hit
CGGCAAGACTATGATTGTGATGTCAATTTGGAAAGTACAGGGCTTGTTAG11.4492753623188406No Hit
CCCAAAGACAGCAGAAGAAAAGAAATAACAAATATCAGACAACTAAATAA11.4492753623188406No Hit
CATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCG11.4492753623188406No Hit
GATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTC11.4492753623188406No Hit
CGAGGGACCTGGGTTGCGTGCTCCTTATGAGAATCTGTCTCTTATACACA11.4492753623188406No Hit
GTAATAAAGTAATTTTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.4492753623188406No Hit
ACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT11.4492753623188406No Hit
GAATGGAACGGAATGTAATGGAATGGAATGGAATGGAATCAACACGAGTG11.4492753623188406No Hit
AAGGAATACAATTGCCTTTGATCCCCTCCTCAAAATTTCATTAACCAGAG11.4492753623188406No Hit
TCCTTCGCCCGAACAGATTTTGCAGGTGCGGCAGCAAGCCGCCGAAGCCG11.4492753623188406No Hit
GGGCAAAAACACTCTCATTTCTGCCAGACGAGGTCTTCACAGCAAGCCTA11.4492753623188406No Hit
GTCCCATCTTTGTGATCCAGGTGACAAATAATCAGTCCCTGGTCCCCACA11.4492753623188406No Hit
GCCTCCGGCAGGAGCTCGCCGTCGGCCAGCGCGGTGAGGAACCTGAAGTA11.4492753623188406No Hit
CCTTACTGCTATGAGCTTTACTGAACGGCTGAAAAACTTGGAAAATAAAA11.4492753623188406No Hit
GAGGAGATCCCGTGCAGCAGCGAGTCGATCGTGGTACTGCCGGTGAGTTC11.4492753623188406No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAA11.4492753623188406No Hit
TTTTTGTACCCTGAAGTGTTTTTTTTATTGCCCTAAGTGATTTCCACAGG11.4492753623188406No Hit
TCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAAGTTTCATAAGGGCT11.4492753623188406No Hit
ATATTCTTGATCTTGTGACCTCGTGATCCGGGAGGTGGAGGTTTGCAGTG11.4492753623188406No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)