FastQCFastQC Report
Tue 10 May 2022
EGAF00003664585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413073
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCTTACCTGTATCTCGTATG52071.260552008966938No Hit
ATACACATCTCCGAGCCCACGAGACTACCTTACCTGTATCTCGTATGCCG32430.7850912550566123No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29160.7059284920583044No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCTTACCTGTATCGCGTATG27300.6609001314537624No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA19620.4749765779898468No Hit
ACACATCTCCGAGCCCACGAGACTACCTTACCTGTATCTCGTATGCCGTC19600.4744924020693679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19000.45996712445499943No Hit
ATACACATCTCCGAGCCCACGAGACTACCTTACCTGTATCGCGTATGCCG17520.4241381063395574No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC15980.3868565604626785No Hit
ACACATCTCCGAGCCCACGAGACTACCTTACCTGTATCGCGTATGCCGTC13140.3181035797546681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12760.3089042372655681No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12380.2997048947764681No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA10330.250076862927376No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT9660.23385696959133132No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC8690.21037443744810236No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7610.18422893774223925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7610.18422893774223925No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA7230.17502959525313927No Hit
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA7180.1738191554519419No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6770.1638935490821235No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC6670.16147266947972874No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA6640.16074640559901035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6340.15348376679182613No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT6090.1474315677858393No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA5860.1418635447003314No Hit
CACATCTCCGAGCCCACGAGACTACCTTACCTGTATCTCGTATGCCGTCT5410.13096958648955512No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC5370.13000123464859722No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA5280.12782244300644197No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT5250.12709617912572355No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT5110.12370694768237091No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT4780.11571804499446829No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA4620.1118446376306367No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA4300.10409782290297356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA4290.10385573494273409No Hit
CATCTCCGAGCCCACGAGACTACCTTACCTGTATCTCGTATGCCGTCTTC4290.10385573494273409RNA PCR Primer, Index 2 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA4190.10143485534033936No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG4170.10095067941986041No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)