FastQCFastQC Report
Tue 10 May 2022
EGAF00003664595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150086
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATG47353.154857881481284No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCGCGTATG28451.895579867542609No Hit
ATACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATGCCG26131.741001825619978No Hit
ATACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCGCGTATGCCG16801.1193582346121558No Hit
ACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATGCCGTC15441.028743520381648No Hit
ACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCGCGTATGCCGTC12150.8095358661034341No Hit
CATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATGCCGTCTTC6220.4144290606718814RNA PCR Primer, Index 14 (95% over 23bp)
CACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATGCCGTCT4220.28117212797995816RNA PCR Primer, Index 14 (95% over 21bp)
CATCTCCGAGCCCACGAGACTGATGCGGGTCCATCGCGTATGCCGTCTTC3580.2385299095185427No Hit
CACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCGCGTATGCCGTCT3480.23186706288394654No Hit
TTATACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATGC2700.17989685913409645No Hit
TTATACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCGCGTATGC2000.1332569326919233No Hit
ACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATGCCGTCTT1880.1252615167304079TruSeq Adapter, Index 16 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTGATGCGGGTCCATCTCGTATGCC1800.11993123942273098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)