FastQCFastQC Report
Tue 10 May 2022
EGAF00003664597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67791
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATG25733.7954890767210987No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCGCGTATG18712.7599533861427035No Hit
ACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATGCCGTC12301.8144001416117181No Hit
ATACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATGCCG10181.5016742635453084No Hit
ACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCGCGTATGCCGTC9081.3394108362466994No Hit
ATACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCGCGTATGCCG7311.0783142305025741No Hit
CATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATGCCGTCTTC5470.8068917702939918RNA PCR Primer, Index 3 (95% over 23bp)
CACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATGCCGTCT3600.5310439438863567TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCGCGTATGCCGTCTTC3080.45433759643610505RNA PCR Primer, Index 20 (95% over 21bp)
CACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCGCGTATGCCGTCT2490.36730539452139666No Hit
ACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATGCCGTCTT1710.2522458733460194RNA PCR Primer, Index 20 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATGC1290.19029074655927777No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTGCCGTG1220.17996489209482083No Hit
ACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCGCGTATGCCGTCTT1010.14898732870145007No Hit
TTATACACATCTCCGAGCCCACGAGACTGCCGTGTAGCCATCGCGTATGC860.12686049770618518No Hit
TCTCCGAGCCCACGAGACTGCCGTGTAGCCATCTCGTATGCCGTCTTCTG800.11800976530807926RNA PCR Primer, Index 20 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)