FastQCFastQC Report
Tue 10 May 2022
EGAF00003664601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003664601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133531
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATG53484.005062494851383No Hit
ATACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATGCCG30332.2713826751840394No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCGCGTATG25131.8819599943084377No Hit
ACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATGCCGTC19551.4640795021380804No Hit
ATACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCGCGTATGCCG14121.0574323565314423No Hit
ACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCGCGTATGCCGTC10750.8050565037332156No Hit
CACATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATGCCGTCT5420.40589825583572353RNA PCR Primer, Index 19 (95% over 21bp)
CATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATGCCGTCTTC4680.35048041278804176RNA PCR Primer, Index 19 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATGC3170.23739805737993425No Hit
CACATCTCCGAGCCCACGAGACTACTGTTTGAATATCGCGTATGCCGTCT3100.2321558289835319No Hit
TATACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATGCC2380.17823576547767933No Hit
CATCTCCGAGCCCACGAGACTACTGTTTGAATATCGCGTATGCCGTCTTC1640.12281792242999753RNA PCR Primer, Index 6 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTACTGTTTGAATATCGCGTATGC1630.1220690326590829No Hit
ACATCTCCGAGCCCACGAGACTACTGTTTGAATATCTCGTATGCCGTCTT1560.11682680426268058Illumina PCR Primer Index 6 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTACTGTT1370.1025978986153028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)